View in EDS

In vivo self-assembly of an intact functional cage protein: Intracellular generation of chimeric ferritins without disassembly-involved damage.

Saved in:
Bibliographic Details
Title: In vivo self-assembly of an intact functional cage protein: Intracellular generation of chimeric ferritins without disassembly-involved damage.
Authors: Kim, Koung Hee1 (AUTHOR), Ki, Mi-Ran1,2 (AUTHOR), Nguyen, Thi Khoa My1 (AUTHOR), Min, Ki Ha1,2 (AUTHOR), Pack, Seung Pil1 (AUTHOR) spack@korea.ac.kr
Source: Journal of Industrial & Engineering Chemistry. Jan2025, Vol. 141, p67-71. 5p.
Subjects: Peptides, Functional analysis, Monomers, Design templates, Nanostructured materials, Ferritin
Abstract: [Display omitted] Ferritin (Fn) is an attractive nano-biomaterial with a hollow structure. Functional chimeric cage-protein Fns (chimera-Fn) can be designed by combining two different monomer subunits. However, unavoidable damage is induced by the severe disassembly conditions required for preparing the Fn monomer. Here, a biocompatible method using a designed dual-expression vector to obtain chimera-Fns without a damage-inducing process is described. Fn monomer and silica-forming peptide (Kpt)-fused Fn monomer are expressed simultaneously and assembled within host-cells to form chimera-Fn of Kpt. Characteristic analysis showed that the chimera-Fns obtained intracellularly were composed of the two monomers in a ratio of 1:1. Morphological and functional analyses determined that the intracellularly-generated chimera-Fns had more intact forms and bioactive functions compared with those produced by chemical-involved disassembly and reassembly. This is the first report about the in vivo self-assembly of chimera-Fn with intact function and hold promise in designing functional templates for various nanomaterial preparations. [ABSTRACT FROM AUTHOR]
Copyright of Journal of Industrial & Engineering Chemistry is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
FullText Text:
  Availability: 0
Header DbId: egs
DbLabel: Engineering Source
An: 181284532
AccessLevel: 6
PubType: Periodical
PubTypeId: serialPeriodical
PreciseRelevancyScore: 0
IllustrationInfo
Items – Name: Title
  Label: Title
  Group: Ti
  Data: In vivo self-assembly of an intact functional cage protein: Intracellular generation of chimeric ferritins without disassembly-involved damage.
– Name: Author
  Label: Authors
  Group: Au
  Data: <searchLink fieldCode="AR" term="%22Kim%2C+Koung+Hee%22">Kim, Koung Hee</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Ki%2C+Mi-Ran%22">Ki, Mi-Ran</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Nguyen%2C+Thi+Khoa+My%22">Nguyen, Thi Khoa My</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Min%2C+Ki+Ha%22">Min, Ki Ha</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Pack%2C+Seung+Pil%22">Pack, Seung Pil</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> spack@korea.ac.kr</i>
– Name: TitleSource
  Label: Source
  Group: Src
  Data: <searchLink fieldCode="JN" term="%22Journal+of+Industrial+%26+Engineering+Chemistry%22">Journal of Industrial & Engineering Chemistry</searchLink>. Jan2025, Vol. 141, p67-71. 5p.
– Name: Subject
  Label: Subjects
  Group: Su
  Data: <searchLink fieldCode="DE" term="%22Peptides%22">Peptides</searchLink><br /><searchLink fieldCode="DE" term="%22Functional+analysis%22">Functional analysis</searchLink><br /><searchLink fieldCode="DE" term="%22Monomers%22">Monomers</searchLink><br /><searchLink fieldCode="DE" term="%22Design+templates%22">Design templates</searchLink><br /><searchLink fieldCode="DE" term="%22Nanostructured+materials%22">Nanostructured materials</searchLink><br /><searchLink fieldCode="DE" term="%22Ferritin%22">Ferritin</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: [Display omitted] Ferritin (Fn) is an attractive nano-biomaterial with a hollow structure. Functional chimeric cage-protein Fns (chimera-Fn) can be designed by combining two different monomer subunits. However, unavoidable damage is induced by the severe disassembly conditions required for preparing the Fn monomer. Here, a biocompatible method using a designed dual-expression vector to obtain chimera-Fns without a damage-inducing process is described. Fn monomer and silica-forming peptide (Kpt)-fused Fn monomer are expressed simultaneously and assembled within host-cells to form chimera-Fn of Kpt. Characteristic analysis showed that the chimera-Fns obtained intracellularly were composed of the two monomers in a ratio of 1:1. Morphological and functional analyses determined that the intracellularly-generated chimera-Fns had more intact forms and bioactive functions compared with those produced by chemical-involved disassembly and reassembly. This is the first report about the in vivo self-assembly of chimera-Fn with intact function and hold promise in designing functional templates for various nanomaterial preparations. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Journal of Industrial & Engineering Chemistry is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
PLink https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=egs&AN=181284532
RecordInfo BibRecord:
  BibEntity:
    Identifiers:
      – Type: doi
        Value: 10.1016/j.jiec.2024.06.040
    Languages:
      – Code: eng
        Text: English
    PhysicalDescription:
      Pagination:
        PageCount: 5
        StartPage: 67
    Subjects:
      – SubjectFull: Peptides
        Type: general
      – SubjectFull: Functional analysis
        Type: general
      – SubjectFull: Monomers
        Type: general
      – SubjectFull: Design templates
        Type: general
      – SubjectFull: Nanostructured materials
        Type: general
      – SubjectFull: Ferritin
        Type: general
    Titles:
      – TitleFull: In vivo self-assembly of an intact functional cage protein: Intracellular generation of chimeric ferritins without disassembly-involved damage.
        Type: main
  BibRelationships:
    HasContributorRelationships:
      – PersonEntity:
          Name:
            NameFull: Kim, Koung Hee
      – PersonEntity:
          Name:
            NameFull: Ki, Mi-Ran
      – PersonEntity:
          Name:
            NameFull: Nguyen, Thi Khoa My
      – PersonEntity:
          Name:
            NameFull: Min, Ki Ha
      – PersonEntity:
          Name:
            NameFull: Pack, Seung Pil
    IsPartOfRelationships:
      – BibEntity:
          Dates:
            – D: 25
              M: 01
              Text: Jan2025
              Type: published
              Y: 2025
          Identifiers:
            – Type: issn-print
              Value: 1226086X
          Numbering:
            – Type: volume
              Value: 141
          Titles:
            – TitleFull: Journal of Industrial & Engineering Chemistry
              Type: main
ResultId 1