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Screening, characterization, and optimization of the fermentation conditions of a novel cellulase‐producing microorganism from soil of Qinghai–Tibet Plateau.

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Title: Screening, characterization, and optimization of the fermentation conditions of a novel cellulase‐producing microorganism from soil of Qinghai–Tibet Plateau.
Authors: Zhao, Longmei1 (AUTHOR) zhaolongmei@126.com, Zhang, Lan1,2 (AUTHOR), Qin, Yaning1,3 (AUTHOR), Li, Wang1 (AUTHOR), Li, Yuanxiao1 (AUTHOR), Cao, Hui1 (AUTHOR), Cao, Pinghua1 (AUTHOR), Ding, Ke1 (AUTHOR), He, Wanling1 (AUTHOR)
Source: Biotechnology & Applied Biochemistry. Dec2024, Vol. 71 Issue 6, p1211-1225. 15p.
Subjects: Corn straw, Agricultural wastes, Cellulase, Extreme environments, Lignocellulose
Geographic Terms: Tibet (China)
Abstract: Cellulases play an important role in the bioconversion of lignocellulose. Microorganisms found in extreme environments are a potentially rich source of cellulases with unique properties. Due to the uniqueness of the environment, the abundant microbial resources in the Qinghai–Tibet Plateau (QTP) are worth being explored. The aim of this study was to isolate and characterize an acidic, mesophilic cellulase‐producing microorganism from QTP. Moreover, the fermentation conditions for cellulase production were optimized for future application of cellulase in the development of lignocellulose biomass. A novel cellulase‐producing strain, Penicillium oxalicum XC10, was isolated from the soil of QTP. The cellulase produced by XC10 was a mesophilic cellulase that exhibited good acid resistance and some cold‐adaptation properties, with maximum activity at pH 4.0 and 40°C. Cellulase activity was significantly enhanced by Na+ (p < 0.05) and inhibited by Mg2+, Ca2+, Cu2+, and Fe3+ (p < 0.05). After optimization, maximum cellulase activity (8.56 U/mL) was increased nearly 10‐fold. Optimal fermentation conditions included an inoculum size of 3% (v/v) in a mixture of corn straw (40 g/L), peptone (5 g/L), and Mg2+ (4 g/L), at pH 4.0, 33°C, and shaking at 200 rpm. The specific properties of the P. oxalicum XC10 cellulase suggest the enzyme may serve as an excellent candidate for the bioconversion and utilization of lignocellulose biomass generated as agricultural and food‐processing wastes. [ABSTRACT FROM AUTHOR]
Copyright of Biotechnology & Applied Biochemistry is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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  Data: Screening, characterization, and optimization of the fermentation conditions of a novel cellulase‐producing microorganism from soil of Qinghai–Tibet Plateau.
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  Data: &lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Zhao%2C+Longmei%22&quot;&gt;Zhao, Longmei&lt;/searchLink&gt;&lt;relatesTo&gt;1&lt;/relatesTo&gt; (AUTHOR)&lt;i&gt; zhaolongmei@126.com&lt;/i&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Zhang%2C+Lan%22&quot;&gt;Zhang, Lan&lt;/searchLink&gt;&lt;relatesTo&gt;1,2&lt;/relatesTo&gt; (AUTHOR)&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Qin%2C+Yaning%22&quot;&gt;Qin, Yaning&lt;/searchLink&gt;&lt;relatesTo&gt;1,3&lt;/relatesTo&gt; (AUTHOR)&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Li%2C+Wang%22&quot;&gt;Li, Wang&lt;/searchLink&gt;&lt;relatesTo&gt;1&lt;/relatesTo&gt; (AUTHOR)&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Li%2C+Yuanxiao%22&quot;&gt;Li, Yuanxiao&lt;/searchLink&gt;&lt;relatesTo&gt;1&lt;/relatesTo&gt; (AUTHOR)&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Cao%2C+Hui%22&quot;&gt;Cao, Hui&lt;/searchLink&gt;&lt;relatesTo&gt;1&lt;/relatesTo&gt; (AUTHOR)&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Cao%2C+Pinghua%22&quot;&gt;Cao, Pinghua&lt;/searchLink&gt;&lt;relatesTo&gt;1&lt;/relatesTo&gt; (AUTHOR)&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Ding%2C+Ke%22&quot;&gt;Ding, Ke&lt;/searchLink&gt;&lt;relatesTo&gt;1&lt;/relatesTo&gt; (AUTHOR)&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22He%2C+Wanling%22&quot;&gt;He, Wanling&lt;/searchLink&gt;&lt;relatesTo&gt;1&lt;/relatesTo&gt; (AUTHOR)
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  Data: &lt;searchLink fieldCode=&quot;JN&quot; term=&quot;%22Biotechnology+%26+Applied+Biochemistry%22&quot;&gt;Biotechnology &amp; Applied Biochemistry&lt;/searchLink&gt;. Dec2024, Vol. 71 Issue 6, p1211-1225. 15p.
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  Data: &lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Corn+straw%22&quot;&gt;Corn straw&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Agricultural+wastes%22&quot;&gt;Agricultural wastes&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Cellulase%22&quot;&gt;Cellulase&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Extreme+environments%22&quot;&gt;Extreme environments&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Lignocellulose%22&quot;&gt;Lignocellulose&lt;/searchLink&gt;
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  Data: &lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Tibet+%28China%29%22&quot;&gt;Tibet (China)&lt;/searchLink&gt;
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Cellulases play an important role in the bioconversion of lignocellulose. Microorganisms found in extreme environments are a potentially rich source of cellulases with unique properties. Due to the uniqueness of the environment, the abundant microbial resources in the Qinghai–Tibet Plateau (QTP) are worth being explored. The aim of this study was to isolate and characterize an acidic, mesophilic cellulase‐producing microorganism from QTP. Moreover, the fermentation conditions for cellulase production were optimized for future application of cellulase in the development of lignocellulose biomass. A novel cellulase‐producing strain, Penicillium oxalicum XC10, was isolated from the soil of QTP. The cellulase produced by XC10 was a mesophilic cellulase that exhibited good acid resistance and some cold‐adaptation properties, with maximum activity at pH 4.0 and 40&#176;C. Cellulase activity was significantly enhanced by Na+ (p &lt; 0.05) and inhibited by Mg2+, Ca2+, Cu2+, and Fe3+ (p &lt; 0.05). After optimization, maximum cellulase activity (8.56 U/mL) was increased nearly 10‐fold. Optimal fermentation conditions included an inoculum size of 3% (v/v) in a mixture of corn straw (40 g/L), peptone (5 g/L), and Mg2+ (4 g/L), at pH 4.0, 33&#176;C, and shaking at 200 rpm. The specific properties of the P. oxalicum XC10 cellulase suggest the enzyme may serve as an excellent candidate for the bioconversion and utilization of lignocellulose biomass generated as agricultural and food‐processing wastes. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
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  Data: &lt;i&gt;Copyright of Biotechnology &amp; Applied Biochemistry is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder&#39;s express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.&lt;/i&gt; (Copyright applies to all Abstracts.)
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        Value: 10.1002/bab.2622
    Languages:
      – Code: eng
        Text: English
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        PageCount: 15
        StartPage: 1211
    Subjects:
      – SubjectFull: Corn straw
        Type: general
      – SubjectFull: Agricultural wastes
        Type: general
      – SubjectFull: Cellulase
        Type: general
      – SubjectFull: Extreme environments
        Type: general
      – SubjectFull: Lignocellulose
        Type: general
      – SubjectFull: Tibet (China)
        Type: general
    Titles:
      – TitleFull: Screening, characterization, and optimization of the fermentation conditions of a novel cellulase‐producing microorganism from soil of Qinghai–Tibet Plateau.
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            NameFull: Zhao, Longmei
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            NameFull: Zhang, Lan
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              M: 12
              Text: Dec2024
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              Y: 2024
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