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Identification of a novel anti‐ROR1 nanobody through phage display and its biochemical characterization.

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Title: Identification of a novel anti‐ROR1 nanobody through phage display and its biochemical characterization.
Authors: Kang, Li1,2 (AUTHOR), Dong, Yingkui1,2 (AUTHOR), Wang, Wanxue2,3 (AUTHOR), Li, Zehua2,4 (AUTHOR), Wang, Yizhuo2,4 (AUTHOR), Yan, Li2,3 (AUTHOR), Yin, Cunlong2,3 (AUTHOR), Zhang, XiaoHui5 (AUTHOR), Dai, Han2 (AUTHOR) daihan@hmfl.ac.cn, Wu, Bo2 (AUTHOR) wubo@hmfl.ac.cn, Zhao, Hongxin2,6 (AUTHOR) zhx@hmfl.ac.cn, Wang, Junfeng1,2 (AUTHOR) junfeng@hmfl.ac.cn
Source: Biotechnology & Applied Biochemistry. Dec2024, Vol. 71 Issue 6, p1226-1234. 9p.
Subjects: Surface plasmon resonance, Complementary DNA, Immunoglobulins, Cancer diagnosis, Cancer treatment
Abstract: In this study, we aimed to develop nanobodies targeting receptor tyrosine kinase‐like orphan receptor 1 (ROR1) for cancer diagnosis and therapy. We immunized alpacas with ROR1, extracted RNA from their blood, and converted it to complementary DNA (cDNA) to amplify the VHH (variable domain of heavy‐chain antibodies) sequence. This sequence was used to construct a phage library with a capacity of 8 ×108. Screening identified a high‐affinity nanobody, HCAbs1, which binds effectively to ROR1. ELISA and surface plasmon resonance analyses revealed HCAbs1's binding affinities to ROR1 at 4.42 and 12.9 nM, respectively. Functional tests showed HCAbs1 could reduce extracellular signal‐regulated kinase (ERK) phosphorylation levels induced by Wnt5a in ROR1‐transfected cells. Our findings highlight the potential of HCAbs1 nanobodies in diagnosing and treating cancers through targeting ROR1. [ABSTRACT FROM AUTHOR]
Copyright of Biotechnology & Applied Biochemistry is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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  Data: Identification of a novel anti‐ROR1 nanobody through phage display and its biochemical characterization.
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  Data: <searchLink fieldCode="AR" term="%22Kang%2C+Li%22">Kang, Li</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Dong%2C+Yingkui%22">Dong, Yingkui</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Wang%2C+Wanxue%22">Wang, Wanxue</searchLink><relatesTo>2,3</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Li%2C+Zehua%22">Li, Zehua</searchLink><relatesTo>2,4</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Wang%2C+Yizhuo%22">Wang, Yizhuo</searchLink><relatesTo>2,4</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Yan%2C+Li%22">Yan, Li</searchLink><relatesTo>2,3</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Yin%2C+Cunlong%22">Yin, Cunlong</searchLink><relatesTo>2,3</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Zhang%2C+XiaoHui%22">Zhang, XiaoHui</searchLink><relatesTo>5</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Dai%2C+Han%22">Dai, Han</searchLink><relatesTo>2</relatesTo> (AUTHOR)<i> daihan@hmfl.ac.cn</i><br /><searchLink fieldCode="AR" term="%22Wu%2C+Bo%22">Wu, Bo</searchLink><relatesTo>2</relatesTo> (AUTHOR)<i> wubo@hmfl.ac.cn</i><br /><searchLink fieldCode="AR" term="%22Zhao%2C+Hongxin%22">Zhao, Hongxin</searchLink><relatesTo>2,6</relatesTo> (AUTHOR)<i> zhx@hmfl.ac.cn</i><br /><searchLink fieldCode="AR" term="%22Wang%2C+Junfeng%22">Wang, Junfeng</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<i> junfeng@hmfl.ac.cn</i>
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  Data: <searchLink fieldCode="JN" term="%22Biotechnology+%26+Applied+Biochemistry%22">Biotechnology & Applied Biochemistry</searchLink>. Dec2024, Vol. 71 Issue 6, p1226-1234. 9p.
– Name: Subject
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  Data: <searchLink fieldCode="DE" term="%22Surface+plasmon+resonance%22">Surface plasmon resonance</searchLink><br /><searchLink fieldCode="DE" term="%22Complementary+DNA%22">Complementary DNA</searchLink><br /><searchLink fieldCode="DE" term="%22Immunoglobulins%22">Immunoglobulins</searchLink><br /><searchLink fieldCode="DE" term="%22Cancer+diagnosis%22">Cancer diagnosis</searchLink><br /><searchLink fieldCode="DE" term="%22Cancer+treatment%22">Cancer treatment</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: In this study, we aimed to develop nanobodies targeting receptor tyrosine kinase‐like orphan receptor 1 (ROR1) for cancer diagnosis and therapy. We immunized alpacas with ROR1, extracted RNA from their blood, and converted it to complementary DNA (cDNA) to amplify the VHH (variable domain of heavy‐chain antibodies) sequence. This sequence was used to construct a phage library with a capacity of 8 ×108. Screening identified a high‐affinity nanobody, HCAbs1, which binds effectively to ROR1. ELISA and surface plasmon resonance analyses revealed HCAbs1's binding affinities to ROR1 at 4.42 and 12.9 nM, respectively. Functional tests showed HCAbs1 could reduce extracellular signal‐regulated kinase (ERK) phosphorylation levels induced by Wnt5a in ROR1‐transfected cells. Our findings highlight the potential of HCAbs1 nanobodies in diagnosing and treating cancers through targeting ROR1. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Biotechnology & Applied Biochemistry is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Value: 10.1002/bab.2623
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        Text: English
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        PageCount: 9
        StartPage: 1226
    Subjects:
      – SubjectFull: Surface plasmon resonance
        Type: general
      – SubjectFull: Complementary DNA
        Type: general
      – SubjectFull: Immunoglobulins
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      – SubjectFull: Cancer diagnosis
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      – SubjectFull: Cancer treatment
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      – TitleFull: Identification of a novel anti‐ROR1 nanobody through phage display and its biochemical characterization.
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            – D: 01
              M: 12
              Text: Dec2024
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