Biocompatibility Analysis of the Silver-Coated Microporous Titanium Implants Manufactured with 3D-Printing Technology.
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| Title: | Biocompatibility Analysis of the Silver-Coated Microporous Titanium Implants Manufactured with 3D-Printing Technology. |
|---|---|
| Authors: | Shevtsov, Maxim1,2,3 (AUTHOR) maxim.shevtsov@tum.de, Pitkin, Emil4 (AUTHOR) emil.pitkin@gmail.com, Combs, Stephanie E.1 (AUTHOR), Yudintceva, Natalia2 (AUTHOR), Nazarov, Denis5 (AUTHOR) dennazar1@yandex.ru, Meulen, Greg Van Der6 (AUTHOR) greg.vandermeulen@movora.com, Preucil, Chris6 (AUTHOR) chris.preucil@movora.com, Akkaoui, Michael7 (AUTHOR) michael@tanury.com, Pitkin, Mark8,9 (AUTHOR) maxim.shevtsov@tum.de |
| Source: | Nanomaterials (2079-4991). Dec2024, Vol. 14 Issue 23, p1876. 20p. |
| Subjects: | Physical vapor deposition, Extracellular matrix, Polymerase chain reaction, Titanium alloys, Osteocalcin, Cell adhesion |
| Abstract: | 3D-printed microporous titanium scaffolds enjoy good biointegration with the residuum's soft and bone tissues, and they promote excellent biomechanical properties in attached prostheses. Implant-associated infection, however, remains a major clinical challenge. Silver-based implant coatings can potentially reduce bacterial growth and inhibit biofilm formation, thereby reducing the risk of periprosthetic infections. In the current study, a 1-µm thick silver coating was prepared on the surface of a 3D-printed microporous titanium alloy with physical vapor deposition (PVD), with a final silver content of 1.00 ± 02 mg/cm2. Cell viability was evaluated with an MTT assay of MC3T3-E1 osteoblasts and human dermal fibroblasts cultured on the surface of the implants, and showed low cytotoxicity for cells during the 14-day follow-up period. Quantitative real-time polymerase chain reaction (RT-PCR) analysis of the relative gene expression of the extracellular matrix components (fibronectin, vitronectin, type I collagen) and cell adhesion markers (α2, α5, αV, β1 integrins) in dermal fibroblasts showed that cell adhesion was not reduced by the silver coating of the microporous implants. An RT-PCR analysis of gene expression related to osteogenic differentiation, including TGF-β1, SMAD4, osteocalcin, osteopontin, and osteonectin in MC3T3-E1 osteoblasts, demonstrated that silver coating did not reduce the osteogenic activity of cells and, to the contrary, enhanced the activity of the TGF-β signaling pathway. For representative sample S5 on day 14, the gene expression levels were 7.15 ± 0.29 (osteonectin), 6.08 ± 0.12 (osteocalcin), and 11.19 ± 0.77 (osteopontin). In conclusion, the data indicate that the silver coating of the microporous titanium implants did not reduce the biointegrative or osteoinductive properties of the titanium scaffold, a finding that argues in favor of applying this coating in designing personalized osseointegrated implants. [ABSTRACT FROM AUTHOR] |
| Copyright of Nanomaterials (2079-4991) is the property of MDPI and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
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| Items | – Name: Title Label: Title Group: Ti Data: Biocompatibility Analysis of the Silver-Coated Microporous Titanium Implants Manufactured with 3D-Printing Technology. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Shevtsov%2C+Maxim%22">Shevtsov, Maxim</searchLink><relatesTo>1,2,3</relatesTo> (AUTHOR)<i> maxim.shevtsov@tum.de</i><br /><searchLink fieldCode="AR" term="%22Pitkin%2C+Emil%22">Pitkin, Emil</searchLink><relatesTo>4</relatesTo> (AUTHOR)<i> emil.pitkin@gmail.com</i><br /><searchLink fieldCode="AR" term="%22Combs%2C+Stephanie+E%2E%22">Combs, Stephanie E.</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Yudintceva%2C+Natalia%22">Yudintceva, Natalia</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Nazarov%2C+Denis%22">Nazarov, Denis</searchLink><relatesTo>5</relatesTo> (AUTHOR)<i> dennazar1@yandex.ru</i><br /><searchLink fieldCode="AR" term="%22Meulen%2C+Greg+Van+Der%22">Meulen, Greg Van Der</searchLink><relatesTo>6</relatesTo> (AUTHOR)<i> greg.vandermeulen@movora.com</i><br /><searchLink fieldCode="AR" term="%22Preucil%2C+Chris%22">Preucil, Chris</searchLink><relatesTo>6</relatesTo> (AUTHOR)<i> chris.preucil@movora.com</i><br /><searchLink fieldCode="AR" term="%22Akkaoui%2C+Michael%22">Akkaoui, Michael</searchLink><relatesTo>7</relatesTo> (AUTHOR)<i> michael@tanury.com</i><br /><searchLink fieldCode="AR" term="%22Pitkin%2C+Mark%22">Pitkin, Mark</searchLink><relatesTo>8,9</relatesTo> (AUTHOR)<i> maxim.shevtsov@tum.de</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Nanomaterials+%282079-4991%29%22">Nanomaterials (2079-4991)</searchLink>. Dec2024, Vol. 14 Issue 23, p1876. 20p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Physical+vapor+deposition%22">Physical vapor deposition</searchLink><br /><searchLink fieldCode="DE" term="%22Extracellular+matrix%22">Extracellular matrix</searchLink><br /><searchLink fieldCode="DE" term="%22Polymerase+chain+reaction%22">Polymerase chain reaction</searchLink><br /><searchLink fieldCode="DE" term="%22Titanium+alloys%22">Titanium alloys</searchLink><br /><searchLink fieldCode="DE" term="%22Osteocalcin%22">Osteocalcin</searchLink><br /><searchLink fieldCode="DE" term="%22Cell+adhesion%22">Cell adhesion</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: 3D-printed microporous titanium scaffolds enjoy good biointegration with the residuum's soft and bone tissues, and they promote excellent biomechanical properties in attached prostheses. Implant-associated infection, however, remains a major clinical challenge. Silver-based implant coatings can potentially reduce bacterial growth and inhibit biofilm formation, thereby reducing the risk of periprosthetic infections. In the current study, a 1-µm thick silver coating was prepared on the surface of a 3D-printed microporous titanium alloy with physical vapor deposition (PVD), with a final silver content of 1.00 ± 02 mg/cm2. Cell viability was evaluated with an MTT assay of MC3T3-E1 osteoblasts and human dermal fibroblasts cultured on the surface of the implants, and showed low cytotoxicity for cells during the 14-day follow-up period. Quantitative real-time polymerase chain reaction (RT-PCR) analysis of the relative gene expression of the extracellular matrix components (fibronectin, vitronectin, type I collagen) and cell adhesion markers (α2, α5, αV, β1 integrins) in dermal fibroblasts showed that cell adhesion was not reduced by the silver coating of the microporous implants. An RT-PCR analysis of gene expression related to osteogenic differentiation, including TGF-β1, SMAD4, osteocalcin, osteopontin, and osteonectin in MC3T3-E1 osteoblasts, demonstrated that silver coating did not reduce the osteogenic activity of cells and, to the contrary, enhanced the activity of the TGF-β signaling pathway. For representative sample S5 on day 14, the gene expression levels were 7.15 ± 0.29 (osteonectin), 6.08 ± 0.12 (osteocalcin), and 11.19 ± 0.77 (osteopontin). In conclusion, the data indicate that the silver coating of the microporous titanium implants did not reduce the biointegrative or osteoinductive properties of the titanium scaffold, a finding that argues in favor of applying this coating in designing personalized osseointegrated implants. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Nanomaterials (2079-4991) is the property of MDPI and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.3390/nano14231876 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 20 StartPage: 1876 Subjects: – SubjectFull: Physical vapor deposition Type: general – SubjectFull: Extracellular matrix Type: general – SubjectFull: Polymerase chain reaction Type: general – SubjectFull: Titanium alloys Type: general – SubjectFull: Osteocalcin Type: general – SubjectFull: Cell adhesion Type: general Titles: – TitleFull: Biocompatibility Analysis of the Silver-Coated Microporous Titanium Implants Manufactured with 3D-Printing Technology. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Shevtsov, Maxim – PersonEntity: Name: NameFull: Pitkin, Emil – PersonEntity: Name: NameFull: Combs, Stephanie E. – PersonEntity: Name: NameFull: Yudintceva, Natalia – PersonEntity: Name: NameFull: Nazarov, Denis – PersonEntity: Name: NameFull: Meulen, Greg Van Der – PersonEntity: Name: NameFull: Preucil, Chris – PersonEntity: Name: NameFull: Akkaoui, Michael – PersonEntity: Name: NameFull: Pitkin, Mark IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 12 Text: Dec2024 Type: published Y: 2024 Identifiers: – Type: issn-print Value: 20794991 Numbering: – Type: volume Value: 14 – Type: issue Value: 23 Titles: – TitleFull: Nanomaterials (2079-4991) Type: main |
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