Phytochemical Investigation and Biological Activities of Ruta chalepensis Methanolic Extract: Antioxidant, Anti‐Inflammatory, Anticollagenase, and Antielastase Properties.
Saved in:
| Title: | Phytochemical Investigation and Biological Activities of Ruta chalepensis Methanolic Extract: Antioxidant, Anti‐Inflammatory, Anticollagenase, and Antielastase Properties. |
|---|---|
| Authors: | Althaher, Arwa R.1 (AUTHOR) a.althaher@zuj.edu.jo, Oran, Sawsan A.2 (AUTHOR), Jarrar, Yazun3 (AUTHOR), Tlili, Nizar (AUTHOR) |
| Source: | Journal of Food Biochemistry. 12/22/2024, Vol. 2024, p1-10. 10p. |
| Subjects: | Collagenases, Elastases, Traditional medicine, Myricetin, Biological assay, Phytochemicals |
| Abstract: | Ruta chalepensis has long been recognized in traditional medicine for its diverse pharmacological properties. The primary aim of this study was to analyze the phytochemical composition and evaluate the antioxidant, anti‐inflammatory, anticollagenase, and antielastase activities of the methanolic extract derived from R. chalepensis. The extract was subjected to phytochemical screening using LC‐MS analysis. For evaluation of the antioxidant activity, 2,2‐diphenyl‐1‐picrylhydrazyl (DPPH) and 2,2′‐azino‐bis (3‐ethylbenzothiazoline‐6‐sulfonic acid) (ABTS) assays were used. The anti‐inflammatory properties of the extract were investigated using a protein denaturation bioassay. Finally, fluorometric screening kits assessed the anticollagenase and antielastase activities. The phytochemical screening identified 33 compounds, the dominant being 3,3′,4,5,7‐pentahydroxyflavone (9.4%), and myricetin (8.9%). The extract showed antioxidant activity, with IC50 values of 30.1 μg/mL for the DPPH assay and 25.4 μg/mL for the ABTS assay. Moreover, the extract displayed significant inhibition of protein denaturation across a range of concentrations. Additionally, the methanolic extract exhibited a dose‐dependent inhibitory effect on collagenase and elastase activities. The highest concentration tested, 5%, achieved 41.7% for collagenase inhibition and 62.4% for elastase inhibition. In summary, the methanolic extract of R. chalepensis showcases promising antioxidant and enzyme inhibitory activities, hinting at its potential for a wide array of therapeutic applications. [ABSTRACT FROM AUTHOR] |
| Copyright of Journal of Food Biochemistry is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
|
Full text is not displayed to guests.
Login for full access.
|
|
| FullText | Links: – Type: pdflink Text: Availability: 1 |
|---|---|
| Header | DbId: egs DbLabel: Engineering Source An: 181824499 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
| IllustrationInfo | |
| Items | – Name: Title Label: Title Group: Ti Data: Phytochemical Investigation and Biological Activities of Ruta chalepensis Methanolic Extract: Antioxidant, Anti‐Inflammatory, Anticollagenase, and Antielastase Properties. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Althaher%2C+Arwa+R%2E%22">Althaher, Arwa R.</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> a.althaher@zuj.edu.jo</i><br /><searchLink fieldCode="AR" term="%22Oran%2C+Sawsan+A%2E%22">Oran, Sawsan A.</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Jarrar%2C+Yazun%22">Jarrar, Yazun</searchLink><relatesTo>3</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Tlili%2C+Nizar%22">Tlili, Nizar</searchLink> (AUTHOR) – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Journal+of+Food+Biochemistry%22">Journal of Food Biochemistry</searchLink>. 12/22/2024, Vol. 2024, p1-10. 10p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Collagenases%22">Collagenases</searchLink><br /><searchLink fieldCode="DE" term="%22Elastases%22">Elastases</searchLink><br /><searchLink fieldCode="DE" term="%22Traditional+medicine%22">Traditional medicine</searchLink><br /><searchLink fieldCode="DE" term="%22Myricetin%22">Myricetin</searchLink><br /><searchLink fieldCode="DE" term="%22Biological+assay%22">Biological assay</searchLink><br /><searchLink fieldCode="DE" term="%22Phytochemicals%22">Phytochemicals</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Ruta chalepensis has long been recognized in traditional medicine for its diverse pharmacological properties. The primary aim of this study was to analyze the phytochemical composition and evaluate the antioxidant, anti‐inflammatory, anticollagenase, and antielastase activities of the methanolic extract derived from R. chalepensis. The extract was subjected to phytochemical screening using LC‐MS analysis. For evaluation of the antioxidant activity, 2,2‐diphenyl‐1‐picrylhydrazyl (DPPH) and 2,2′‐azino‐bis (3‐ethylbenzothiazoline‐6‐sulfonic acid) (ABTS) assays were used. The anti‐inflammatory properties of the extract were investigated using a protein denaturation bioassay. Finally, fluorometric screening kits assessed the anticollagenase and antielastase activities. The phytochemical screening identified 33 compounds, the dominant being 3,3′,4,5,7‐pentahydroxyflavone (9.4%), and myricetin (8.9%). The extract showed antioxidant activity, with IC50 values of 30.1 μg/mL for the DPPH assay and 25.4 μg/mL for the ABTS assay. Moreover, the extract displayed significant inhibition of protein denaturation across a range of concentrations. Additionally, the methanolic extract exhibited a dose‐dependent inhibitory effect on collagenase and elastase activities. The highest concentration tested, 5%, achieved 41.7% for collagenase inhibition and 62.4% for elastase inhibition. In summary, the methanolic extract of R. chalepensis showcases promising antioxidant and enzyme inhibitory activities, hinting at its potential for a wide array of therapeutic applications. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Journal of Food Biochemistry is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
| PLink | https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=egs&AN=181824499 |
| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1155/jfbc/9921218 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 10 StartPage: 1 Subjects: – SubjectFull: Collagenases Type: general – SubjectFull: Elastases Type: general – SubjectFull: Traditional medicine Type: general – SubjectFull: Myricetin Type: general – SubjectFull: Biological assay Type: general – SubjectFull: Phytochemicals Type: general Titles: – TitleFull: Phytochemical Investigation and Biological Activities of Ruta chalepensis Methanolic Extract: Antioxidant, Anti‐Inflammatory, Anticollagenase, and Antielastase Properties. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Althaher, Arwa R. – PersonEntity: Name: NameFull: Oran, Sawsan A. – PersonEntity: Name: NameFull: Jarrar, Yazun – PersonEntity: Name: NameFull: Tlili, Nizar IsPartOfRelationships: – BibEntity: Dates: – D: 22 M: 12 Text: 12/22/2024 Type: published Y: 2024 Identifiers: – Type: issn-print Value: 01458884 Numbering: – Type: volume Value: 2024 Titles: – TitleFull: Journal of Food Biochemistry Type: main |
| ResultId | 1 |
