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Mitochondrial oxidative phosphorylation capacity in skeletal muscle measured by ultrafast Z‐spectroscopy (UFZ) MRI at 3T.

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Title: Mitochondrial oxidative phosphorylation capacity in skeletal muscle measured by ultrafast Z‐spectroscopy (UFZ) MRI at 3T.
Authors: Ju, Licheng1,2 (AUTHOR), Schär, Michael2 (AUTHOR), Wang, Kexin1,3 (AUTHOR), Li, Anna1 (AUTHOR), Wu, Yihan1,3 (AUTHOR), Samuel, T. Jake4 (AUTHOR), Ganji, Sandeep5,6 (AUTHOR), van Zijl, Peter C. M.1,2 (AUTHOR), Yadav, Nirbhay N.1,2 (AUTHOR), Weiss, Robert G.2,4 (AUTHOR), Xu, Jiadi1,2 (AUTHOR) jxu37@jh.edu
Source: Magnetic Resonance in Medicine. Mar2025, Vol. 93 Issue 3, p1273-1284. 12p.
Subjects: Magnetization transfer, Skeletal muscle, Oxidative phosphorylation, Creatine kinase, Calf muscles
Abstract: Purpose: To investigate the feasibility of rapid CEST MRI acquisition for evaluating oxidative phosphorylation (OXPHOS) in human skeletal muscle at 3T, utilizing ultrafast Z‐spectroscopy (UFZ) combined with MRI and the Polynomial and Lorentzian line‐shape Fitting (PLOF) technique. Methods: UFZ MRI on muscle was evaluated with turbo spin echo (TSE) and 3D EPI readouts. Five healthy subjects performed in‐magnet plantar flexion exercise (PFE) and subsequent changes of amide, PCr, and partial PCr mixed Cr (Cr+) CEST dynamic signals post‐exercise were enabled by PLOF fitting. PCr/Cr CEST signal was further refined through pH correction by using the ratios between PCr/Cr and amide signals, named PCAR/CAR, respectively. Results: UFZ MRI with TSE readout significantly reduces acquisition time, achieving a temporal resolution of <50 s for collecting high‐resolution Z‐spectra. Following PFE, the recovery/decay times (τ) for both PCr and Cr in the gastrocnemius muscle of the calf were notably longer when determined using PCr/Cr CEST compared to those after pH correction with amideCEST, namely τCr+$$ {\tau}_{Cr^{+}} $$ = 87.1 ± 15.8 s and τPCr$$ {\tau}_{PCr} $$ = 98.1 ± 20.4 s versus τCAR$$ {\tau}_{CAR} $$ = 32.9 ± 19.7 s and τPCAR$$ {\tau}_{PCAR} $$ = 43.0 ± 13.0 s, respectively. τPCr$$ {\tau}_{PCr} $$ obtained via 31P MRS (τPCr$$ {\tau}_{PCr} $$ = 50.3 ± 6.2 s) closely resemble those obtained from pH‐corrected PCr/Cr CEST signals. Conclusion: The outcomes suggest potential of UFZ MRI as a robust tool for non‐invasive assessment of mitochondrial function in skeletal muscles. pH correction is critical for the reliable OXPHOS measurement by CEST. [ABSTRACT FROM AUTHOR]
Copyright of Magnetic Resonance in Medicine is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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  Data: Mitochondrial oxidative phosphorylation capacity in skeletal muscle measured by ultrafast Z‐spectroscopy (UFZ) MRI at 3T.
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  Data: &lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Ju%2C+Licheng%22&quot;&gt;Ju, Licheng&lt;/searchLink&gt;&lt;relatesTo&gt;1,2&lt;/relatesTo&gt; (AUTHOR)&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Sch&#228;r%2C+Michael%22&quot;&gt;Sch&#228;r, Michael&lt;/searchLink&gt;&lt;relatesTo&gt;2&lt;/relatesTo&gt; (AUTHOR)&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Wang%2C+Kexin%22&quot;&gt;Wang, Kexin&lt;/searchLink&gt;&lt;relatesTo&gt;1,3&lt;/relatesTo&gt; (AUTHOR)&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Li%2C+Anna%22&quot;&gt;Li, Anna&lt;/searchLink&gt;&lt;relatesTo&gt;1&lt;/relatesTo&gt; (AUTHOR)&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Wu%2C+Yihan%22&quot;&gt;Wu, Yihan&lt;/searchLink&gt;&lt;relatesTo&gt;1,3&lt;/relatesTo&gt; (AUTHOR)&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Samuel%2C+T%2E+Jake%22&quot;&gt;Samuel, T. Jake&lt;/searchLink&gt;&lt;relatesTo&gt;4&lt;/relatesTo&gt; (AUTHOR)&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Ganji%2C+Sandeep%22&quot;&gt;Ganji, Sandeep&lt;/searchLink&gt;&lt;relatesTo&gt;5,6&lt;/relatesTo&gt; (AUTHOR)&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22van+Zijl%2C+Peter+C%2E+M%2E%22&quot;&gt;van Zijl, Peter C. M.&lt;/searchLink&gt;&lt;relatesTo&gt;1,2&lt;/relatesTo&gt; (AUTHOR)&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Yadav%2C+Nirbhay+N%2E%22&quot;&gt;Yadav, Nirbhay N.&lt;/searchLink&gt;&lt;relatesTo&gt;1,2&lt;/relatesTo&gt; (AUTHOR)&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Weiss%2C+Robert+G%2E%22&quot;&gt;Weiss, Robert G.&lt;/searchLink&gt;&lt;relatesTo&gt;2,4&lt;/relatesTo&gt; (AUTHOR)&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Xu%2C+Jiadi%22&quot;&gt;Xu, Jiadi&lt;/searchLink&gt;&lt;relatesTo&gt;1,2&lt;/relatesTo&gt; (AUTHOR)&lt;i&gt; jxu37@jh.edu&lt;/i&gt;
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  Data: &lt;searchLink fieldCode=&quot;JN&quot; term=&quot;%22Magnetic+Resonance+in+Medicine%22&quot;&gt;Magnetic Resonance in Medicine&lt;/searchLink&gt;. Mar2025, Vol. 93 Issue 3, p1273-1284. 12p.
– Name: Subject
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  Data: &lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Magnetization+transfer%22&quot;&gt;Magnetization transfer&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Skeletal+muscle%22&quot;&gt;Skeletal muscle&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Oxidative+phosphorylation%22&quot;&gt;Oxidative phosphorylation&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Creatine+kinase%22&quot;&gt;Creatine kinase&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Calf+muscles%22&quot;&gt;Calf muscles&lt;/searchLink&gt;
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Purpose: To investigate the feasibility of rapid CEST MRI acquisition for evaluating oxidative phosphorylation (OXPHOS) in human skeletal muscle at 3T, utilizing ultrafast Z‐spectroscopy (UFZ) combined with MRI and the Polynomial and Lorentzian line‐shape Fitting (PLOF) technique. Methods: UFZ MRI on muscle was evaluated with turbo spin echo (TSE) and 3D EPI readouts. Five healthy subjects performed in‐magnet plantar flexion exercise (PFE) and subsequent changes of amide, PCr, and partial PCr mixed Cr (Cr+) CEST dynamic signals post‐exercise were enabled by PLOF fitting. PCr/Cr CEST signal was further refined through pH correction by using the ratios between PCr/Cr and amide signals, named PCAR/CAR, respectively. Results: UFZ MRI with TSE readout significantly reduces acquisition time, achieving a temporal resolution of &lt;50 s for collecting high‐resolution Z‐spectra. Following PFE, the recovery/decay times (τ) for both PCr and Cr in the gastrocnemius muscle of the calf were notably longer when determined using PCr/Cr CEST compared to those after pH correction with amideCEST, namely τCr+$$ {\tau}_{Cr^{+}} $$ = 87.1 &#177; 15.8 s and τPCr$$ {\tau}_{PCr} $$ = 98.1 &#177; 20.4 s versus τCAR$$ {\tau}_{CAR} $$ = 32.9 &#177; 19.7 s and τPCAR$$ {\tau}_{PCAR} $$ = 43.0 &#177; 13.0 s, respectively. τPCr$$ {\tau}_{PCr} $$ obtained via 31P MRS (τPCr$$ {\tau}_{PCr} $$ = 50.3 &#177; 6.2 s) closely resemble those obtained from pH‐corrected PCr/Cr CEST signals. Conclusion: The outcomes suggest potential of UFZ MRI as a robust tool for non‐invasive assessment of mitochondrial function in skeletal muscles. pH correction is critical for the reliable OXPHOS measurement by CEST. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
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  Data: &lt;i&gt;Copyright of Magnetic Resonance in Medicine is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder&#39;s express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.&lt;/i&gt; (Copyright applies to all Abstracts.)
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      – Type: doi
        Value: 10.1002/mrm.30354
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      – Code: eng
        Text: English
    PhysicalDescription:
      Pagination:
        PageCount: 12
        StartPage: 1273
    Subjects:
      – SubjectFull: Magnetization transfer
        Type: general
      – SubjectFull: Skeletal muscle
        Type: general
      – SubjectFull: Oxidative phosphorylation
        Type: general
      – SubjectFull: Creatine kinase
        Type: general
      – SubjectFull: Calf muscles
        Type: general
    Titles:
      – TitleFull: Mitochondrial oxidative phosphorylation capacity in skeletal muscle measured by ultrafast Z‐spectroscopy (UFZ) MRI at 3T.
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            – D: 01
              M: 03
              Text: Mar2025
              Type: published
              Y: 2025
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