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Extracellular matrix-enriched polymeric scaffolds as a substrate for hepatocyte cultures: in vitro and in vivo studies

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Title: Extracellular matrix-enriched polymeric scaffolds as a substrate for hepatocyte cultures: in vitro and in vivo studies
Authors: Zavan, B.1 barbara.zavan@unipd.it, Brun, P.1, Vindigni, V.2, Amadori, A.3, Habeler, W.3, Pontisso, P.4, Montemurro, D.5, Abatangelo, G.1, Cortivo, R.1
Source: Biomaterials. Dec2005, Vol. 26 Issue 34, p7038-7045. 8p.
Subjects: Liver cells, Hyaluronic acid, Connective tissues, Mucopolysaccharides
Abstract: Abstract: Tissue engineering is a promising approach to developing hepatic tissue suitable for the functional replacement of a failing liver. The aim of the present study was to investigate whether an extracellular cell matrix obtained from fibroblasts-cultured within scaffolds of hyaluronic acid (HYAFF™) could influence the proliferation rate and survival of rat hepatocytes both during long-term culture and after in vivo transplantation. Cultures were evaluated by histological and morphological analysis, a proliferation assay and metabolic activity (albumin secretion). Hepatocytes cultured in extracellular matrix-enriched scaffolds exhibited a round cellular morphology and re-established cell–cell contacts, growing into aggregates of several cells along and/or among fibers in the fabric. Hepatocytes were able to secrete albumin up to 14 days in culture. In vivo results demonstrated the biocompatibility of HYAFF-11TM implanted in nude mice, in which hepatocytes maintained small well-organised aggregates until the 35th day. In conclusion, the presence of a fibroblast-secreted extracellular matrix improved the biological properties of the hyaluronan scaffold, favoring the survival and morphological integrity of hepatocytes in vitro and in vivo. [Copyright &y& Elsevier]
Copyright of Biomaterials is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
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DbLabel: Engineering Source
An: 18205300
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PubType: Academic Journal
PubTypeId: academicJournal
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  Data: Extracellular matrix-enriched polymeric scaffolds as a substrate for hepatocyte cultures: in vitro and in vivo studies
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  Data: <searchLink fieldCode="AR" term="%22Zavan%2C+B%2E%22">Zavan, B.</searchLink><relatesTo>1</relatesTo><i> barbara.zavan@unipd.it</i><br /><searchLink fieldCode="AR" term="%22Brun%2C+P%2E%22">Brun, P.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Vindigni%2C+V%2E%22">Vindigni, V.</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Amadori%2C+A%2E%22">Amadori, A.</searchLink><relatesTo>3</relatesTo><br /><searchLink fieldCode="AR" term="%22Habeler%2C+W%2E%22">Habeler, W.</searchLink><relatesTo>3</relatesTo><br /><searchLink fieldCode="AR" term="%22Pontisso%2C+P%2E%22">Pontisso, P.</searchLink><relatesTo>4</relatesTo><br /><searchLink fieldCode="AR" term="%22Montemurro%2C+D%2E%22">Montemurro, D.</searchLink><relatesTo>5</relatesTo><br /><searchLink fieldCode="AR" term="%22Abatangelo%2C+G%2E%22">Abatangelo, G.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Cortivo%2C+R%2E%22">Cortivo, R.</searchLink><relatesTo>1</relatesTo>
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  Data: <searchLink fieldCode="JN" term="%22Biomaterials%22">Biomaterials</searchLink>. Dec2005, Vol. 26 Issue 34, p7038-7045. 8p.
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  Data: <searchLink fieldCode="DE" term="%22Liver+cells%22">Liver cells</searchLink><br /><searchLink fieldCode="DE" term="%22Hyaluronic+acid%22">Hyaluronic acid</searchLink><br /><searchLink fieldCode="DE" term="%22Connective+tissues%22">Connective tissues</searchLink><br /><searchLink fieldCode="DE" term="%22Mucopolysaccharides%22">Mucopolysaccharides</searchLink>
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  Data: Abstract: Tissue engineering is a promising approach to developing hepatic tissue suitable for the functional replacement of a failing liver. The aim of the present study was to investigate whether an extracellular cell matrix obtained from fibroblasts-cultured within scaffolds of hyaluronic acid (HYAFF™) could influence the proliferation rate and survival of rat hepatocytes both during long-term culture and after in vivo transplantation. Cultures were evaluated by histological and morphological analysis, a proliferation assay and metabolic activity (albumin secretion). Hepatocytes cultured in extracellular matrix-enriched scaffolds exhibited a round cellular morphology and re-established cell–cell contacts, growing into aggregates of several cells along and/or among fibers in the fabric. Hepatocytes were able to secrete albumin up to 14 days in culture. In vivo results demonstrated the biocompatibility of HYAFF-11TM implanted in nude mice, in which hepatocytes maintained small well-organised aggregates until the 35th day. In conclusion, the presence of a fibroblast-secreted extracellular matrix improved the biological properties of the hyaluronan scaffold, favoring the survival and morphological integrity of hepatocytes in vitro and in vivo. [Copyright &y& Elsevier]
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  Data: <i>Copyright of Biomaterials is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Value: 10.1016/j.biomaterials.2005.04.067
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      – SubjectFull: Connective tissues
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              Text: Dec2005
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