Curcumin-mediated antimicrobial photodynamic therapy for inactivating Mycobacterium abscessus: a promising approach for non-tuberculous mycobacterial skin infections.
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| Title: | Curcumin-mediated antimicrobial photodynamic therapy for inactivating Mycobacterium abscessus: a promising approach for non-tuberculous mycobacterial skin infections. |
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| Authors: | Dang, Yang1 (AUTHOR), Yang, Runping2 (AUTHOR), Jia, Tao1 (AUTHOR), Liu, Chengcheng3 (AUTHOR) liuchengcheng@mail.xjtu.edu.cn, Geng, Songmei1 (AUTHOR) gengsongmei73@163.com |
| Source: | Lasers in Medical Science. 1/7/2025, Vol. 40 Issue 1, p1-11. 11p. |
| Subjects: | Mycobacterial diseases, Photodynamic therapy, Skin infections, Reactive oxygen species, Transmission electron microscopy |
| Abstract: | Non-tuberculous mycobacterial skin infection lead to complex and lengthy treatment cycles. Antimicrobial photodynamic therapy (aPDT) is an emerging promising approach for treating infections. This study aims to assess the effects of aPDT using curcumin as a photosensitizer (PS) on non-tuberculous mycobacteria, Mycobacterium abscessus, a subtype that has become common in dermatology in recent years. The antimicrobial efficacy of curcumin-mediated aPDT against M.abscessus ATCC19977 was evaluated using the colony-forming unit (CFU) assay. Reactive oxygen species (ROS) production and cell membrane integrity were assessed using H2DCFDA and propidium iodide staining, respectively. Cellular damage was further examined through transmission electron microscopy (TEM). Curcumin-mediated aPDT showed significant antimicrobial effects on M.abscessus in a concentration- and light-dose-dependent manner. Complete inactivation of M.abscessus was achieved with 100 μM curcumin and 48 J cm−2 light exposure. ROS levels and cell membrane damage increased with higher curcumin concentrations, with fluorescence intensities of ROS and damaged cells increasing by 5.32 and 9.53 times, respectively, compared to untreated controls. TEM confirmed extensive cell wall and cytoplasmic damage in treated cells. This study demonstrates that M.abscessus could be effectively inactivated by curcumin in the presence of light. Therefore, curcumin-mediated aPDT shows promise as a treatment for M.abscessus. [ABSTRACT FROM AUTHOR] |
| Copyright of Lasers in Medical Science is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
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| Header | DbId: egs DbLabel: Engineering Source An: 182077194 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Curcumin-mediated antimicrobial photodynamic therapy for inactivating Mycobacterium abscessus: a promising approach for non-tuberculous mycobacterial skin infections. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Dang%2C+Yang%22">Dang, Yang</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Yang%2C+Runping%22">Yang, Runping</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Jia%2C+Tao%22">Jia, Tao</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Liu%2C+Chengcheng%22">Liu, Chengcheng</searchLink><relatesTo>3</relatesTo> (AUTHOR)<i> liuchengcheng@mail.xjtu.edu.cn</i><br /><searchLink fieldCode="AR" term="%22Geng%2C+Songmei%22">Geng, Songmei</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> gengsongmei73@163.com</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Lasers+in+Medical+Science%22">Lasers in Medical Science</searchLink>. 1/7/2025, Vol. 40 Issue 1, p1-11. 11p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Mycobacterial+diseases%22">Mycobacterial diseases</searchLink><br /><searchLink fieldCode="DE" term="%22Photodynamic+therapy%22">Photodynamic therapy</searchLink><br /><searchLink fieldCode="DE" term="%22Skin+infections%22">Skin infections</searchLink><br /><searchLink fieldCode="DE" term="%22Reactive+oxygen+species%22">Reactive oxygen species</searchLink><br /><searchLink fieldCode="DE" term="%22Transmission+electron+microscopy%22">Transmission electron microscopy</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Non-tuberculous mycobacterial skin infection lead to complex and lengthy treatment cycles. Antimicrobial photodynamic therapy (aPDT) is an emerging promising approach for treating infections. This study aims to assess the effects of aPDT using curcumin as a photosensitizer (PS) on non-tuberculous mycobacteria, Mycobacterium abscessus, a subtype that has become common in dermatology in recent years. The antimicrobial efficacy of curcumin-mediated aPDT against M.abscessus ATCC19977 was evaluated using the colony-forming unit (CFU) assay. Reactive oxygen species (ROS) production and cell membrane integrity were assessed using H2DCFDA and propidium iodide staining, respectively. Cellular damage was further examined through transmission electron microscopy (TEM). Curcumin-mediated aPDT showed significant antimicrobial effects on M.abscessus in a concentration- and light-dose-dependent manner. Complete inactivation of M.abscessus was achieved with 100 μM curcumin and 48 J cm−2 light exposure. ROS levels and cell membrane damage increased with higher curcumin concentrations, with fluorescence intensities of ROS and damaged cells increasing by 5.32 and 9.53 times, respectively, compared to untreated controls. TEM confirmed extensive cell wall and cytoplasmic damage in treated cells. This study demonstrates that M.abscessus could be effectively inactivated by curcumin in the presence of light. Therefore, curcumin-mediated aPDT shows promise as a treatment for M.abscessus. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Lasers in Medical Science is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1007/s10103-024-04263-0 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 11 StartPage: 1 Subjects: – SubjectFull: Mycobacterial diseases Type: general – SubjectFull: Photodynamic therapy Type: general – SubjectFull: Skin infections Type: general – SubjectFull: Reactive oxygen species Type: general – SubjectFull: Transmission electron microscopy Type: general Titles: – TitleFull: Curcumin-mediated antimicrobial photodynamic therapy for inactivating Mycobacterium abscessus: a promising approach for non-tuberculous mycobacterial skin infections. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Dang, Yang – PersonEntity: Name: NameFull: Yang, Runping – PersonEntity: Name: NameFull: Jia, Tao – PersonEntity: Name: NameFull: Liu, Chengcheng – PersonEntity: Name: NameFull: Geng, Songmei IsPartOfRelationships: – BibEntity: Dates: – D: 07 M: 01 Text: 1/7/2025 Type: published Y: 2025 Identifiers: – Type: issn-print Value: 02688921 Numbering: – Type: volume Value: 40 – Type: issue Value: 1 Titles: – TitleFull: Lasers in Medical Science Type: main |
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