Characterization of Composite Agarose–Collagen Hydrogels for Chondrocyte Culture: Characterization of Composite Agarose–Collagen Hydrogels...: C. Zigan et al.
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| Title: | Characterization of Composite Agarose–Collagen Hydrogels for Chondrocyte Culture: Characterization of Composite Agarose–Collagen Hydrogels...: C. Zigan et al. |
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| Authors: | Zigan, Clarisse1 (AUTHOR), Benito Alston, Claudia1 (AUTHOR), Chatterjee, Aritra1,2 (AUTHOR), Solorio, Luis1 (AUTHOR), Chan, Deva D.1,3 (AUTHOR) chand@purdue.edu |
| Source: | Annals of Biomedical Engineering. Jan2025, Vol. 53 Issue 1, p120-132. 13p. |
| Subjects: | Mechanotransduction (Cytology), Life sciences, Biopolymers, Cytology, Extracellular matrix |
| Abstract: | To elucidate the mechanisms of cellular mechanotransduction, it is necessary to employ biomaterials that effectively merge biofunctionality with appropriate mechanical characteristics. Agarose and collagen separately are common biopolymers used in cartilage mechanobiology and mechanotransduction studies but lack features that make them ideal for functional engineered cartilage. In this study, agarose is blended with collagen type I to create hydrogels with final concentrations of 4% w/v or 2% w/v agarose with 2 mg/mL collagen. We hypothesized that the addition of collagen into a high-concentration agarose hydrogel does not diminish mechanical properties. Acellular and cell-laden studies were completed to assess rheologic and compressive properties, contraction, and structural homogeneity in addition to cell proliferation and sulfated glycosaminoglycan production. Over 21 days in culture, cellular 4% agarose–2 mg/mL collagen I hydrogels seeded with primary murine chondrocytes displayed structural and bulk mechanical behaviors that did not significantly alter from 4% agarose-only hydrogels, cell proliferation, and continual glycosaminoglycan production, indicating promise toward the development of an effective hydrogel for chondrocyte mechanotransduction and mechanobiology studies. [ABSTRACT FROM AUTHOR] |
| Copyright of Annals of Biomedical Engineering is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
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| Header | DbId: egs DbLabel: Engineering Source An: 182565043 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Characterization of Composite Agarose–Collagen Hydrogels for Chondrocyte Culture: Characterization of Composite Agarose–Collagen Hydrogels...: C. Zigan et al. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Zigan%2C+Clarisse%22">Zigan, Clarisse</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Benito+Alston%2C+Claudia%22">Benito Alston, Claudia</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Chatterjee%2C+Aritra%22">Chatterjee, Aritra</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Solorio%2C+Luis%22">Solorio, Luis</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Chan%2C+Deva+D%2E%22">Chan, Deva D.</searchLink><relatesTo>1,3</relatesTo> (AUTHOR)<i> chand@purdue.edu</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Annals+of+Biomedical+Engineering%22">Annals of Biomedical Engineering</searchLink>. Jan2025, Vol. 53 Issue 1, p120-132. 13p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Mechanotransduction+%28Cytology%29%22">Mechanotransduction (Cytology)</searchLink><br /><searchLink fieldCode="DE" term="%22Life+sciences%22">Life sciences</searchLink><br /><searchLink fieldCode="DE" term="%22Biopolymers%22">Biopolymers</searchLink><br /><searchLink fieldCode="DE" term="%22Cytology%22">Cytology</searchLink><br /><searchLink fieldCode="DE" term="%22Extracellular+matrix%22">Extracellular matrix</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: To elucidate the mechanisms of cellular mechanotransduction, it is necessary to employ biomaterials that effectively merge biofunctionality with appropriate mechanical characteristics. Agarose and collagen separately are common biopolymers used in cartilage mechanobiology and mechanotransduction studies but lack features that make them ideal for functional engineered cartilage. In this study, agarose is blended with collagen type I to create hydrogels with final concentrations of 4% w/v or 2% w/v agarose with 2 mg/mL collagen. We hypothesized that the addition of collagen into a high-concentration agarose hydrogel does not diminish mechanical properties. Acellular and cell-laden studies were completed to assess rheologic and compressive properties, contraction, and structural homogeneity in addition to cell proliferation and sulfated glycosaminoglycan production. Over 21 days in culture, cellular 4% agarose–2 mg/mL collagen I hydrogels seeded with primary murine chondrocytes displayed structural and bulk mechanical behaviors that did not significantly alter from 4% agarose-only hydrogels, cell proliferation, and continual glycosaminoglycan production, indicating promise toward the development of an effective hydrogel for chondrocyte mechanotransduction and mechanobiology studies. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Annals of Biomedical Engineering is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1007/s10439-024-03613-x Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 13 StartPage: 120 Subjects: – SubjectFull: Mechanotransduction (Cytology) Type: general – SubjectFull: Life sciences Type: general – SubjectFull: Biopolymers Type: general – SubjectFull: Cytology Type: general – SubjectFull: Extracellular matrix Type: general Titles: – TitleFull: Characterization of Composite Agarose–Collagen Hydrogels for Chondrocyte Culture: Characterization of Composite Agarose–Collagen Hydrogels...: C. Zigan et al. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Zigan, Clarisse – PersonEntity: Name: NameFull: Benito Alston, Claudia – PersonEntity: Name: NameFull: Chatterjee, Aritra – PersonEntity: Name: NameFull: Solorio, Luis – PersonEntity: Name: NameFull: Chan, Deva D. IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 01 Text: Jan2025 Type: published Y: 2025 Identifiers: – Type: issn-print Value: 00906964 Numbering: – Type: volume Value: 53 – Type: issue Value: 1 Titles: – TitleFull: Annals of Biomedical Engineering Type: main |
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