View in EDS

Direct Implementation of MSn Using Frequency Scanning Collision Induced Dissociation in a Digital Ion Trap Mass Spectrometer.

Saved in:
Bibliographic Details
Title: Direct Implementation of MSn Using Frequency Scanning Collision Induced Dissociation in a Digital Ion Trap Mass Spectrometer.
Authors: Ren, Jie1 (AUTHOR), Chen, Pingping1 (AUTHOR), Zhang, Yunjing1 (AUTHOR), He, Xingli1 (AUTHOR), Li, Lingfeng1,2 (AUTHOR) lingfengli@suda.edu.cn, Li, Peng1,2 (AUTHOR) lipengsuda@suda.edu.cn
Source: Journal of the American Society for Mass Spectrometry. 2/5/2024, Vol. 36 Issue 2, p309-317. 9p.
Abstract: Tandem mass spectrometry (MSn) is one of the most effective methods to obtain the structures of organic molecules, enabling the observation of multigenerational ion fragments. Collision-induced dissociation (CID) is currently the most mature technique for mass spectrometry analysis. Ion trap mass spectrometry (ITMS) is favored for on-site detection field, due to its ability of MSn analysis with a single trap and its small size. However, conventional MSn analysis in ITMS requires repeated isolation and excitation processes multiple times, causing high complexity of the entire scanning process. Additionally, the fragment ion detection in ITMS is limited by low-mass cutoff (LMCO) and the weak fragmentation yield. In this study, a method named reverse scanning-collision induced dissociation (RS-CID) is proposed, which involves increasing RF and AC frequencies while maintaining RF voltage constant during the CID process. Twelve representative illegal drugs were analyzed adopting this method, enhancing the intensities of low-mass fragment ions compared to conventional dissociation method. Moreover, experimental results with ketamine and methamphetamine show that RS-CID effectively reduces the LMCO effect and slightly improves CID efficiency. It also enables direct acquisition of their multigeneration fragment ions spectra in a single sequence of ion injection, cooling, isolation, RS-CID, cooling, mass scanning and empty. The experiments to distinguish between the isomers ab-4en-pinaca and adb-3en-butinaca as well as the isomeric compounds 5f-cumyl-pegaclone and cumyl-pipetinaca were also successful by this method. In summary, RS-CID enables MSn analysis in a single sequence and improves the low-mass fragment ions intensity. It can simplify workflows, achieve faster analysis and provide more valuable mass spectral information. [ABSTRACT FROM AUTHOR]
Copyright of Journal of the American Society for Mass Spectrometry is the property of American Chemical Society and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
FullText Text:
  Availability: 0
Header DbId: egs
DbLabel: Engineering Source
An: 182829319
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 0
IllustrationInfo
Items – Name: Title
  Label: Title
  Group: Ti
  Data: Direct Implementation of MS<superscript>n</superscript> Using Frequency Scanning Collision Induced Dissociation in a Digital Ion Trap Mass Spectrometer.
– Name: Author
  Label: Authors
  Group: Au
  Data: <searchLink fieldCode="AR" term="%22Ren%2C+Jie%22">Ren, Jie</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Chen%2C+Pingping%22">Chen, Pingping</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Zhang%2C+Yunjing%22">Zhang, Yunjing</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22He%2C+Xingli%22">He, Xingli</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Li%2C+Lingfeng%22">Li, Lingfeng</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<i> lingfengli@suda.edu.cn</i><br /><searchLink fieldCode="AR" term="%22Li%2C+Peng%22">Li, Peng</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<i> lipengsuda@suda.edu.cn</i>
– Name: TitleSource
  Label: Source
  Group: Src
  Data: <searchLink fieldCode="JN" term="%22Journal+of+the+American+Society+for+Mass+Spectrometry%22">Journal of the American Society for Mass Spectrometry</searchLink>. 2/5/2024, Vol. 36 Issue 2, p309-317. 9p.
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Tandem mass spectrometry (MSn) is one of the most effective methods to obtain the structures of organic molecules, enabling the observation of multigenerational ion fragments. Collision-induced dissociation (CID) is currently the most mature technique for mass spectrometry analysis. Ion trap mass spectrometry (ITMS) is favored for on-site detection field, due to its ability of MSn analysis with a single trap and its small size. However, conventional MSn analysis in ITMS requires repeated isolation and excitation processes multiple times, causing high complexity of the entire scanning process. Additionally, the fragment ion detection in ITMS is limited by low-mass cutoff (LMCO) and the weak fragmentation yield. In this study, a method named reverse scanning-collision induced dissociation (RS-CID) is proposed, which involves increasing RF and AC frequencies while maintaining RF voltage constant during the CID process. Twelve representative illegal drugs were analyzed adopting this method, enhancing the intensities of low-mass fragment ions compared to conventional dissociation method. Moreover, experimental results with ketamine and methamphetamine show that RS-CID effectively reduces the LMCO effect and slightly improves CID efficiency. It also enables direct acquisition of their multigeneration fragment ions spectra in a single sequence of ion injection, cooling, isolation, RS-CID, cooling, mass scanning and empty. The experiments to distinguish between the isomers ab-4en-pinaca and adb-3en-butinaca as well as the isomeric compounds 5f-cumyl-pegaclone and cumyl-pipetinaca were also successful by this method. In summary, RS-CID enables MSn analysis in a single sequence and improves the low-mass fragment ions intensity. It can simplify workflows, achieve faster analysis and provide more valuable mass spectral information. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Journal of the American Society for Mass Spectrometry is the property of American Chemical Society and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
PLink https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=egs&AN=182829319
RecordInfo BibRecord:
  BibEntity:
    Identifiers:
      – Type: doi
        Value: 10.1021/jasms.4c00395
    Languages:
      – Code: eng
        Text: English
    PhysicalDescription:
      Pagination:
        PageCount: 9
        StartPage: 309
    Titles:
      – TitleFull: Direct Implementation of MSn Using Frequency Scanning Collision Induced Dissociation in a Digital Ion Trap Mass Spectrometer.
        Type: main
  BibRelationships:
    HasContributorRelationships:
      – PersonEntity:
          Name:
            NameFull: Ren, Jie
      – PersonEntity:
          Name:
            NameFull: Chen, Pingping
      – PersonEntity:
          Name:
            NameFull: Zhang, Yunjing
      – PersonEntity:
          Name:
            NameFull: He, Xingli
      – PersonEntity:
          Name:
            NameFull: Li, Lingfeng
      – PersonEntity:
          Name:
            NameFull: Li, Peng
    IsPartOfRelationships:
      – BibEntity:
          Dates:
            – D: 05
              M: 02
              Text: 2/5/2024
              Type: published
              Y: 2025
          Identifiers:
            – Type: issn-print
              Value: 10440305
          Numbering:
            – Type: volume
              Value: 36
            – Type: issue
              Value: 2
          Titles:
            – TitleFull: Journal of the American Society for Mass Spectrometry
              Type: main
ResultId 1