Immobilization of antivenom polyvalent antibodies on MWCNTs@WO3-Co3O4 capped with 3-mercaptopropionic acid for developing impedimetric immunosensor for snake venom detection.
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| Title: | Immobilization of antivenom polyvalent antibodies on MWCNTs@WO3-Co3O4 capped with 3-mercaptopropionic acid for developing impedimetric immunosensor for snake venom detection. |
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| Authors: | Shaheen, Sana1 (AUTHOR), Fatima, Batool1 (AUTHOR), Hussain, Dilshad2 (AUTHOR), Imran, Muhammad3,4 (AUTHOR), Najam, Amina5 (AUTHOR), Najam-ul-Haq, Muhammad6 (AUTHOR) |
| Source: | Microchemical Journal. Feb2025, Vol. 209, pN.PAG-N.PAG. 1p. |
| Subjects: | Multiwalled carbon nanotubes, Carbon electrodes, Intravenous therapy, Antivenins, Point-of-care testing, Snake venom, Venom |
| Abstract: | [Display omitted] • Novel immunosensor is designed for snake venom detection in real biological samples. • MWCNTs@WO 3 -Co 3 O 4 nanocomposite demonstrates enhanced selectivity and sensitivity. • This immunosensor shows wide linear range from 5 to 50 ng/mL and LODs of 0.1 ng/mL and 0.5 ng/mL. • Designed sensor has great potential for convenient point-of-care (POC) testing. Intravenous administration of anti-venoms is effective against snake venoms. In the present study, an immunosensor is developed through a novel method involving a combination of MWCNTs@WO 3 -Co 3 O 4 with linker 3-mercaptopropionic acid (3-MPA) and antivenom polyvalent antibodies. The newly developed immunosensor immoblized on glassy carbon electrode (GCE) to selectively and sensitively detect snake venom via electrochemical techniques. The electrochemical results show high conductivity, signal amplification, enhanced selectivity, and improved sensitivity. The electrochemical performance of MWCNTs-COOH, MWCNTs@WO 3 , and MWCNTs@WO 3 -Co 3 O 4 are compared. The immunosensors' detection response is investigated at varying analyte concentrations and pH via DPV, LSV, and EIS, demonstrating excellent detection outcomes for snake venom. This immunosensor shows a wide linear range from 5 to 50 ng/mL and limits of detection (LODs) of 0.1 ng/mL and 0.5 ng/mL from DPV and LSV, respectively. The MWCNTs@WO 3 -Co 3 O 4 based immunosensor is stable and reproducible and can detect snake venoms in real biological samples and can be integrated to point of care (POC) devices. [ABSTRACT FROM AUTHOR] |
| Copyright of Microchemical Journal is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Text: Availability: 0 |
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| Header | DbId: egs DbLabel: Engineering Source An: 182875567 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Immobilization of antivenom polyvalent antibodies on MWCNTs@WO3-Co3O4 capped with 3-mercaptopropionic acid for developing impedimetric immunosensor for snake venom detection. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Shaheen%2C+Sana%22">Shaheen, Sana</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Fatima%2C+Batool%22">Fatima, Batool</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Hussain%2C+Dilshad%22">Hussain, Dilshad</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Imran%2C+Muhammad%22">Imran, Muhammad</searchLink><relatesTo>3,4</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Najam%2C+Amina%22">Najam, Amina</searchLink><relatesTo>5</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Najam-ul-Haq%2C+Muhammad%22">Najam-ul-Haq, Muhammad</searchLink><relatesTo>6</relatesTo> (AUTHOR) – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Microchemical+Journal%22">Microchemical Journal</searchLink>. Feb2025, Vol. 209, pN.PAG-N.PAG. 1p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Multiwalled+carbon+nanotubes%22">Multiwalled carbon nanotubes</searchLink><br /><searchLink fieldCode="DE" term="%22Carbon+electrodes%22">Carbon electrodes</searchLink><br /><searchLink fieldCode="DE" term="%22Intravenous+therapy%22">Intravenous therapy</searchLink><br /><searchLink fieldCode="DE" term="%22Antivenins%22">Antivenins</searchLink><br /><searchLink fieldCode="DE" term="%22Point-of-care+testing%22">Point-of-care testing</searchLink><br /><searchLink fieldCode="DE" term="%22Snake+venom%22">Snake venom</searchLink><br /><searchLink fieldCode="DE" term="%22Venom%22">Venom</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: [Display omitted] • Novel immunosensor is designed for snake venom detection in real biological samples. • MWCNTs@WO 3 -Co 3 O 4 nanocomposite demonstrates enhanced selectivity and sensitivity. • This immunosensor shows wide linear range from 5 to 50 ng/mL and LODs of 0.1 ng/mL and 0.5 ng/mL. • Designed sensor has great potential for convenient point-of-care (POC) testing. Intravenous administration of anti-venoms is effective against snake venoms. In the present study, an immunosensor is developed through a novel method involving a combination of MWCNTs@WO 3 -Co 3 O 4 with linker 3-mercaptopropionic acid (3-MPA) and antivenom polyvalent antibodies. The newly developed immunosensor immoblized on glassy carbon electrode (GCE) to selectively and sensitively detect snake venom via electrochemical techniques. The electrochemical results show high conductivity, signal amplification, enhanced selectivity, and improved sensitivity. The electrochemical performance of MWCNTs-COOH, MWCNTs@WO 3 , and MWCNTs@WO 3 -Co 3 O 4 are compared. The immunosensors' detection response is investigated at varying analyte concentrations and pH via DPV, LSV, and EIS, demonstrating excellent detection outcomes for snake venom. This immunosensor shows a wide linear range from 5 to 50 ng/mL and limits of detection (LODs) of 0.1 ng/mL and 0.5 ng/mL from DPV and LSV, respectively. The MWCNTs@WO 3 -Co 3 O 4 based immunosensor is stable and reproducible and can detect snake venoms in real biological samples and can be integrated to point of care (POC) devices. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Microchemical Journal is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1016/j.microc.2025.112797 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 1 StartPage: N.PAG Subjects: – SubjectFull: Multiwalled carbon nanotubes Type: general – SubjectFull: Carbon electrodes Type: general – SubjectFull: Intravenous therapy Type: general – SubjectFull: Antivenins Type: general – SubjectFull: Point-of-care testing Type: general – SubjectFull: Snake venom Type: general – SubjectFull: Venom Type: general Titles: – TitleFull: Immobilization of antivenom polyvalent antibodies on MWCNTs@WO3-Co3O4 capped with 3-mercaptopropionic acid for developing impedimetric immunosensor for snake venom detection. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Shaheen, Sana – PersonEntity: Name: NameFull: Fatima, Batool – PersonEntity: Name: NameFull: Hussain, Dilshad – PersonEntity: Name: NameFull: Imran, Muhammad – PersonEntity: Name: NameFull: Najam, Amina – PersonEntity: Name: NameFull: Najam-ul-Haq, Muhammad IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 02 Text: Feb2025 Type: published Y: 2025 Identifiers: – Type: issn-print Value: 0026265X Numbering: – Type: volume Value: 209 Titles: – TitleFull: Microchemical Journal Type: main |
| ResultId | 1 |
