Immobilization of antivenom polyvalent antibodies on MWCNTs@WO3-Co3O4 capped with 3-mercaptopropionic acid for developing impedimetric immunosensor for snake venom detection.
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| Title: | Immobilization of antivenom polyvalent antibodies on MWCNTs@WO3-Co3O4 capped with 3-mercaptopropionic acid for developing impedimetric immunosensor for snake venom detection. |
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| Authors: | Shaheen, Sana1 (AUTHOR), Fatima, Batool1 (AUTHOR), Hussain, Dilshad2 (AUTHOR), Imran, Muhammad3,4 (AUTHOR), Najam, Amina5 (AUTHOR), Najam-ul-Haq, Muhammad6 (AUTHOR) |
| Source: | Microchemical Journal. Feb2025, Vol. 209, pN.PAG-N.PAG. 1p. |
| Subjects: | Multiwalled carbon nanotubes, Carbon electrodes, Intravenous therapy, Antivenins, Point-of-care testing, Snake venom, Venom |
| Abstract: | [Display omitted] • Novel immunosensor is designed for snake venom detection in real biological samples. • MWCNTs@WO 3 -Co 3 O 4 nanocomposite demonstrates enhanced selectivity and sensitivity. • This immunosensor shows wide linear range from 5 to 50 ng/mL and LODs of 0.1 ng/mL and 0.5 ng/mL. • Designed sensor has great potential for convenient point-of-care (POC) testing. Intravenous administration of anti-venoms is effective against snake venoms. In the present study, an immunosensor is developed through a novel method involving a combination of MWCNTs@WO 3 -Co 3 O 4 with linker 3-mercaptopropionic acid (3-MPA) and antivenom polyvalent antibodies. The newly developed immunosensor immoblized on glassy carbon electrode (GCE) to selectively and sensitively detect snake venom via electrochemical techniques. The electrochemical results show high conductivity, signal amplification, enhanced selectivity, and improved sensitivity. The electrochemical performance of MWCNTs-COOH, MWCNTs@WO 3 , and MWCNTs@WO 3 -Co 3 O 4 are compared. The immunosensors' detection response is investigated at varying analyte concentrations and pH via DPV, LSV, and EIS, demonstrating excellent detection outcomes for snake venom. This immunosensor shows a wide linear range from 5 to 50 ng/mL and limits of detection (LODs) of 0.1 ng/mL and 0.5 ng/mL from DPV and LSV, respectively. The MWCNTs@WO 3 -Co 3 O 4 based immunosensor is stable and reproducible and can detect snake venoms in real biological samples and can be integrated to point of care (POC) devices. [ABSTRACT FROM AUTHOR] |
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| Database: | Engineering Source |
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