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Oral mucosa cues for regeneration using Photobiomodulation.

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Title: Oral mucosa cues for regeneration using Photobiomodulation.
Authors: Fassarela Marquiore, Larissa1 (AUTHOR) larissamarquiore@gmail.com, Ferrer Oliveira, Rafaela1 (AUTHOR) odontoferrer@gmail.com, Almeida Queiroz Ferreira, Luiza1 (AUTHOR) luizaalmeidaqf@gmail.com, Gomes, Natália Aparecida1 (AUTHOR) natalia.gomes11@hotmail.com, Martins Olavo de Souza, Milena1 (AUTHOR) milenaolavoacadl@gmail.com, Gomes Lucardians, Beatriz1 (AUTHOR) beatrizlucardians@gmail.com, Reis Cruvinel, João Victor1 (AUTHOR) joaovictorrcruvinel@gmail.com, Morais Perdigão, Ana Luísa1 (AUTHOR) anamoraisp@hotmail.com, Alves Mesquita, Ricardo1 (AUTHOR) ramesquita@ufmg.br, Silva, Tarcília Aparecida1 (AUTHOR) silva.tarcilia@gmail.com, Santos Bernardes, Sara1 (AUTHOR) sarabernardes@ufmg.br, Gonçalves Diniz, Marina1 (AUTHOR) marinadiniz@gmail.com, Alves Diniz, Ivana Marcia1 (AUTHOR) ivanadiniz@ymail.com
Source: Lasers in Medical Science. 2/17/2025, Vol. 40 Issue 1, p1-12. 12p.
Subjects: Vascular endothelial growth factor receptors, Vascular endothelial growth factor antagonists, Photobiomodulation therapy, Oral mucosa, Phototherapy
Abstract: Photobiomodulation therapy (PBMT) is gaining recognition as an effective method for repairing damaged tissues, particularly in skin and mucosa injuries. Despite its growing use, there remains a significant gap in understanding the interaction of ectomesenchymal components during light therapy. This study explored various near-infrared light parameters on stressed skin keratinocytes (KC) cultured alone or in association with oral fibroblasts (FB) using a transwell system. Reactive oxygen species (ROS) levels were quantified, alongside observations of cell viability/proliferation, migration, and pro-resolutive gene expression in KC cultivated conventionally (at 1, 5, and 50 J/cm2; 1.4, 7 and, 70 s; 0.03, 0.14, and 1.4 J, respectively; 0.71 W/cm2; 20 mW) and co-cultured with FB (at 5 J/cm2). Murine tongue fragments were also treated at 5 J/cm2 for three days. The results revealed increased ROS levels in stressed cells. PBMT enhanced cell viability/proliferation and migration of KC in vitro, while pro-inflammatory cytokines were predominantly suppressed following irradiation. All energy densities and culture models showed upregulation of amphiregulin. In the co-culture system, PBMT notably upregulated interleukin 1 - receptor antagonist and vascular endothelial growth factor genes while downregulating transforming growth factor-β1 expression. In ex vivo tongue fragments, PBMT significantly increased lamina propria thickness and spindle-shaped cell counts compared to non-irradiated samples. Overall, near-infrared PBMT demonstrated pro-regenerative effects by modulating inflammation. The activation of pro-resolutive molecules and the dampening of cytokines may favor early tissue remodeling in the dermis counterpart. [ABSTRACT FROM AUTHOR]
Copyright of Lasers in Medical Science is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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  Data: Oral mucosa cues for regeneration using Photobiomodulation.
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  Data: <searchLink fieldCode="AR" term="%22Fassarela+Marquiore%2C+Larissa%22">Fassarela Marquiore, Larissa</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> larissamarquiore@gmail.com</i><br /><searchLink fieldCode="AR" term="%22Ferrer+Oliveira%2C+Rafaela%22">Ferrer Oliveira, Rafaela</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> odontoferrer@gmail.com</i><br /><searchLink fieldCode="AR" term="%22Almeida+Queiroz+Ferreira%2C+Luiza%22">Almeida Queiroz Ferreira, Luiza</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> luizaalmeidaqf@gmail.com</i><br /><searchLink fieldCode="AR" term="%22Gomes%2C+Natália+Aparecida%22">Gomes, Natália Aparecida</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> natalia.gomes11@hotmail.com</i><br /><searchLink fieldCode="AR" term="%22Martins+Olavo+de+Souza%2C+Milena%22">Martins Olavo de Souza, Milena</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> milenaolavoacadl@gmail.com</i><br /><searchLink fieldCode="AR" term="%22Gomes+Lucardians%2C+Beatriz%22">Gomes Lucardians, Beatriz</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> beatrizlucardians@gmail.com</i><br /><searchLink fieldCode="AR" term="%22Reis+Cruvinel%2C+João+Victor%22">Reis Cruvinel, João Victor</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> joaovictorrcruvinel@gmail.com</i><br /><searchLink fieldCode="AR" term="%22Morais+Perdigão%2C+Ana+Luísa%22">Morais Perdigão, Ana Luísa</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> anamoraisp@hotmail.com</i><br /><searchLink fieldCode="AR" term="%22Alves+Mesquita%2C+Ricardo%22">Alves Mesquita, Ricardo</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> ramesquita@ufmg.br</i><br /><searchLink fieldCode="AR" term="%22Silva%2C+Tarcília+Aparecida%22">Silva, Tarcília Aparecida</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> silva.tarcilia@gmail.com</i><br /><searchLink fieldCode="AR" term="%22Santos+Bernardes%2C+Sara%22">Santos Bernardes, Sara</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> sarabernardes@ufmg.br</i><br /><searchLink fieldCode="AR" term="%22Gonçalves+Diniz%2C+Marina%22">Gonçalves Diniz, Marina</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> marinadiniz@gmail.com</i><br /><searchLink fieldCode="AR" term="%22Alves+Diniz%2C+Ivana+Marcia%22">Alves Diniz, Ivana Marcia</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> ivanadiniz@ymail.com</i>
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  Data: <searchLink fieldCode="JN" term="%22Lasers+in+Medical+Science%22">Lasers in Medical Science</searchLink>. 2/17/2025, Vol. 40 Issue 1, p1-12. 12p.
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  Data: <searchLink fieldCode="DE" term="%22Vascular+endothelial+growth+factor+receptors%22">Vascular endothelial growth factor receptors</searchLink><br /><searchLink fieldCode="DE" term="%22Vascular+endothelial+growth+factor+antagonists%22">Vascular endothelial growth factor antagonists</searchLink><br /><searchLink fieldCode="DE" term="%22Photobiomodulation+therapy%22">Photobiomodulation therapy</searchLink><br /><searchLink fieldCode="DE" term="%22Oral+mucosa%22">Oral mucosa</searchLink><br /><searchLink fieldCode="DE" term="%22Phototherapy%22">Phototherapy</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Photobiomodulation therapy (PBMT) is gaining recognition as an effective method for repairing damaged tissues, particularly in skin and mucosa injuries. Despite its growing use, there remains a significant gap in understanding the interaction of ectomesenchymal components during light therapy. This study explored various near-infrared light parameters on stressed skin keratinocytes (KC) cultured alone or in association with oral fibroblasts (FB) using a transwell system. Reactive oxygen species (ROS) levels were quantified, alongside observations of cell viability/proliferation, migration, and pro-resolutive gene expression in KC cultivated conventionally (at 1, 5, and 50 J/cm2; 1.4, 7 and, 70 s; 0.03, 0.14, and 1.4 J, respectively; 0.71 W/cm2; 20 mW) and co-cultured with FB (at 5 J/cm2). Murine tongue fragments were also treated at 5 J/cm2 for three days. The results revealed increased ROS levels in stressed cells. PBMT enhanced cell viability/proliferation and migration of KC in vitro, while pro-inflammatory cytokines were predominantly suppressed following irradiation. All energy densities and culture models showed upregulation of amphiregulin. In the co-culture system, PBMT notably upregulated interleukin 1 - receptor antagonist and vascular endothelial growth factor genes while downregulating transforming growth factor-β1 expression. In ex vivo tongue fragments, PBMT significantly increased lamina propria thickness and spindle-shaped cell counts compared to non-irradiated samples. Overall, near-infrared PBMT demonstrated pro-regenerative effects by modulating inflammation. The activation of pro-resolutive molecules and the dampening of cytokines may favor early tissue remodeling in the dermis counterpart. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
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  Data: <i>Copyright of Lasers in Medical Science is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Value: 10.1007/s10103-025-04343-9
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        Text: English
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      – SubjectFull: Photobiomodulation therapy
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      – SubjectFull: Oral mucosa
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      – SubjectFull: Phototherapy
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