Oral mucosa cues for regeneration using Photobiomodulation.
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| Title: | Oral mucosa cues for regeneration using Photobiomodulation. |
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| Authors: | Fassarela Marquiore, Larissa1 (AUTHOR) larissamarquiore@gmail.com, Ferrer Oliveira, Rafaela1 (AUTHOR) odontoferrer@gmail.com, Almeida Queiroz Ferreira, Luiza1 (AUTHOR) luizaalmeidaqf@gmail.com, Gomes, Natália Aparecida1 (AUTHOR) natalia.gomes11@hotmail.com, Martins Olavo de Souza, Milena1 (AUTHOR) milenaolavoacadl@gmail.com, Gomes Lucardians, Beatriz1 (AUTHOR) beatrizlucardians@gmail.com, Reis Cruvinel, João Victor1 (AUTHOR) joaovictorrcruvinel@gmail.com, Morais Perdigão, Ana Luísa1 (AUTHOR) anamoraisp@hotmail.com, Alves Mesquita, Ricardo1 (AUTHOR) ramesquita@ufmg.br, Silva, Tarcília Aparecida1 (AUTHOR) silva.tarcilia@gmail.com, Santos Bernardes, Sara1 (AUTHOR) sarabernardes@ufmg.br, Gonçalves Diniz, Marina1 (AUTHOR) marinadiniz@gmail.com, Alves Diniz, Ivana Marcia1 (AUTHOR) ivanadiniz@ymail.com |
| Source: | Lasers in Medical Science. 2/17/2025, Vol. 40 Issue 1, p1-12. 12p. |
| Subjects: | Vascular endothelial growth factor receptors, Vascular endothelial growth factor antagonists, Photobiomodulation therapy, Oral mucosa, Phototherapy |
| Abstract: | Photobiomodulation therapy (PBMT) is gaining recognition as an effective method for repairing damaged tissues, particularly in skin and mucosa injuries. Despite its growing use, there remains a significant gap in understanding the interaction of ectomesenchymal components during light therapy. This study explored various near-infrared light parameters on stressed skin keratinocytes (KC) cultured alone or in association with oral fibroblasts (FB) using a transwell system. Reactive oxygen species (ROS) levels were quantified, alongside observations of cell viability/proliferation, migration, and pro-resolutive gene expression in KC cultivated conventionally (at 1, 5, and 50 J/cm2; 1.4, 7 and, 70 s; 0.03, 0.14, and 1.4 J, respectively; 0.71 W/cm2; 20 mW) and co-cultured with FB (at 5 J/cm2). Murine tongue fragments were also treated at 5 J/cm2 for three days. The results revealed increased ROS levels in stressed cells. PBMT enhanced cell viability/proliferation and migration of KC in vitro, while pro-inflammatory cytokines were predominantly suppressed following irradiation. All energy densities and culture models showed upregulation of amphiregulin. In the co-culture system, PBMT notably upregulated interleukin 1 - receptor antagonist and vascular endothelial growth factor genes while downregulating transforming growth factor-β1 expression. In ex vivo tongue fragments, PBMT significantly increased lamina propria thickness and spindle-shaped cell counts compared to non-irradiated samples. Overall, near-infrared PBMT demonstrated pro-regenerative effects by modulating inflammation. The activation of pro-resolutive molecules and the dampening of cytokines may favor early tissue remodeling in the dermis counterpart. [ABSTRACT FROM AUTHOR] |
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| Database: | Engineering Source |
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