Antimicrobial photodynamic therapy using gold nanoparticles conjugated with 1,9-dimethyl-methylene blue for treating Staphylococcus skin infections.
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| Title: | Antimicrobial photodynamic therapy using gold nanoparticles conjugated with 1,9-dimethyl-methylene blue for treating Staphylococcus skin infections. |
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| Authors: | Alves de Brito Júnior, Anildo1 (AUTHOR) junioranildo02@gmail.com, Louro Crugeira, Pedro Jorge1 (AUTHOR) pedrocrugeira@yahoo.com.br, Reis Costa, Wellington Luis1 (AUTHOR) wreiscosta@gmail.com, Barbosa, Andressa Vollono1 (AUTHOR) andressavollono@gmail.com, Pires Sampaio de Oliveira, Susana Carla1 (AUTHOR) susana.oliveira@uniruy.edu.br, Teixeira Cangussu, Maria Cristina1 (AUTHOR) cristinatcangussu@gmail.com, Nunes dos Santos, Jean1 (AUTHOR) jeanpatol@gmail.com, Monteiro Azevedo, Juliana1,2 (AUTHOR) jscmonteiro@uefs.br, Barbosa Pinheiro, Antonio Luiz1 (AUTHOR) albp@ufba.br |
| Source: | Lasers in Medical Science. 2/19/2025, Vol. 40 Issue 1, p1-8. 8p. |
| Subjects: | Staphylococcal diseases, Gold nanoparticles, Photodynamic therapy, Skin injuries, Skin infections |
| Abstract: | This study aims to assess the effectiveness of Antimicrobial Photodynamic Therapy (aPDT) using phenothiazine photosensitizer (PS) combined with gold nanoparticles (DMMB-AuNPs) in treating S. aureus-contaminated wounds on the dorsum of rats. Twelve Wistar rats were divided into four groups: Control, LED, DMMB-AuNPs, and DMMB-AuNPs + LED. DMMB-AuNPs were prepared using DMMB (300 ng/mL) and 5 nm diameter AuNPs (~ 5.5E + 13 particles/mL). The light source was a red LED (12 J/cm2, λ630 ± 20 nm, CW, 125 mW, 192s). Following the procedures, samples were plated on an agar medium specific to S. aureus to count the colony-forming units (CFU/mL). Statistical analysis using unidirectional ANOVA and Tukey's test revealed that the aPDT group (DMMB-AuNPs + LED) exhibited a 99.999% (5 log reduction) microbial reduction with a significant statistical difference (p < 0.0001) compared to the Control group, demonstrating a bactericidal effect. The in vivo findings unequivocally demonstrate the effectiveness of DMMB-AuNPs-mediated aPDT as an alternative therapy for staphylococcal infections. Highlights: aPDT on S. aureus is enhanced by using a phenothiazine photosensitizer (DMMB) combining with gold nanoparticles (AuNPs). aPDT exhibited a 99.999% microbial reduction in surgical skin wounds contaminated with Staphylococcal bacteria. The effectiveness of DMMB-AuNPs-mediated aPDT demonstrate its potential as a compelling alternative therapy for staphylococcal infections. [ABSTRACT FROM AUTHOR] |
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| Database: | Engineering Source |
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