Isolation and Characterization of Neoscytalidium dimidiatum ITD-G6 from Agave durangensis Leaves for Enhanced Cellulase Production in Solid-State Cultivation.
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| Title: | Isolation and Characterization of Neoscytalidium dimidiatum ITD-G6 from Agave durangensis Leaves for Enhanced Cellulase Production in Solid-State Cultivation. |
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| Authors: | Contreras-Hernández, M. G.1,2 (AUTHOR) mgorety@itdurango.edu.mx, Rutiaga-Quiñones, O. M.1 (AUTHOR) omrutiaga@itdurango.edu.mx, Mares-Rodríguez, F. J.1 (AUTHOR) fmaresr@uanl.edu.mx, Irigoyen-Campuzano, J. R.3 (AUTHOR) jose.irigoyen@cimav.edu.mx, Cordero-Soto, I. N.1,2 (AUTHOR) Itza.cordero@itdurango.edu.mx, Aréchiga-Carvajal, E. T.4 (AUTHOR) elva.arechigacr@uanl.edu.mx |
| Source: | BioEnergy Research. 2/20/2025, Vol. 18 Issue 1, p1-13. 13p. |
| Subjects: | Elongation factors (Biochemistry), Life sciences, Biotechnology, Identification of fungi, Biomass conversion, Solid-state fermentation |
| Abstract: | The cellulase production strategy considers the selection of microorganisms and the best experimental conditions for fermentation. This work describes the molecular identification of a fungus isolated from Agave durangensis, employing the internal transcribed spacer 18 S rRNA gene, located in the small subunit 28 S rRNA gene, belonging to a larger subunit, elongation factor 1-α, and the β-tubulin sequences. The best experimental conditions for improving cellulase production using A. durangensis leaves as a solid-state fermentation substrate were also evaluated. A profile of enzymatic activity of endoglucanase (Endo-β), exoglucanase (Exo-β), and β-glucosidase (β-g) obtained at 96 h is presented. Sequences used in the identification analysis of the isolated fungus identified it as a phytopathogen species of Neoscytalidium dimidiatum ITD-G6, belonging to the Botryosphaeriaceae family. To the authors' awareness, this is the first report where leaves of A. durangensis are hosts of this microorganism. The maximum enzymatic activities were obtained at 72 h (2.78 ± 0.015 IU/mL) and 96 h (3.44 ± 0.017 IU/mL), under 37 °C, pH = 4.5, and 70% humidity. At these conditions, the cellulolytic activity of the three enzymes was as follows: Endo-β (0.0547 ± 0.0167 IU/mL), Exo-β (0.0163 ± 0.001 IU/mL), and β-g (0.0692 ± 0.07 IU/mL). The data presented suggest that the isolated fungus has biotechnological potential as a source of cellulases, contributing to the field of little-studied phytopathogens, owing to the feasibility of biomass conversion. [ABSTRACT FROM AUTHOR] |
| Copyright of BioEnergy Research is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
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| Header | DbId: egs DbLabel: Engineering Source An: 183132500 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Isolation and Characterization of Neoscytalidium dimidiatum ITD-G6 from Agave durangensis Leaves for Enhanced Cellulase Production in Solid-State Cultivation. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Contreras-Hernández%2C+M%2E+G%2E%22">Contreras-Hernández, M. G.</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<i> mgorety@itdurango.edu.mx</i><br /><searchLink fieldCode="AR" term="%22Rutiaga-Quiñones%2C+O%2E+M%2E%22">Rutiaga-Quiñones, O. M.</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> omrutiaga@itdurango.edu.mx</i><br /><searchLink fieldCode="AR" term="%22Mares-Rodríguez%2C+F%2E+J%2E%22">Mares-Rodríguez, F. J.</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> fmaresr@uanl.edu.mx</i><br /><searchLink fieldCode="AR" term="%22Irigoyen-Campuzano%2C+J%2E+R%2E%22">Irigoyen-Campuzano, J. R.</searchLink><relatesTo>3</relatesTo> (AUTHOR)<i> jose.irigoyen@cimav.edu.mx</i><br /><searchLink fieldCode="AR" term="%22Cordero-Soto%2C+I%2E+N%2E%22">Cordero-Soto, I. N.</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<i> Itza.cordero@itdurango.edu.mx</i><br /><searchLink fieldCode="AR" term="%22Aréchiga-Carvajal%2C+E%2E+T%2E%22">Aréchiga-Carvajal, E. T.</searchLink><relatesTo>4</relatesTo> (AUTHOR)<i> elva.arechigacr@uanl.edu.mx</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22BioEnergy+Research%22">BioEnergy Research</searchLink>. 2/20/2025, Vol. 18 Issue 1, p1-13. 13p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Elongation+factors+%28Biochemistry%29%22">Elongation factors (Biochemistry)</searchLink><br /><searchLink fieldCode="DE" term="%22Life+sciences%22">Life sciences</searchLink><br /><searchLink fieldCode="DE" term="%22Biotechnology%22">Biotechnology</searchLink><br /><searchLink fieldCode="DE" term="%22Identification+of+fungi%22">Identification of fungi</searchLink><br /><searchLink fieldCode="DE" term="%22Biomass+conversion%22">Biomass conversion</searchLink><br /><searchLink fieldCode="DE" term="%22Solid-state+fermentation%22">Solid-state fermentation</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: The cellulase production strategy considers the selection of microorganisms and the best experimental conditions for fermentation. This work describes the molecular identification of a fungus isolated from Agave durangensis, employing the internal transcribed spacer 18 S rRNA gene, located in the small subunit 28 S rRNA gene, belonging to a larger subunit, elongation factor 1-α, and the β-tubulin sequences. The best experimental conditions for improving cellulase production using A. durangensis leaves as a solid-state fermentation substrate were also evaluated. A profile of enzymatic activity of endoglucanase (Endo-β), exoglucanase (Exo-β), and β-glucosidase (β-g) obtained at 96 h is presented. Sequences used in the identification analysis of the isolated fungus identified it as a phytopathogen species of Neoscytalidium dimidiatum ITD-G6, belonging to the Botryosphaeriaceae family. To the authors' awareness, this is the first report where leaves of A. durangensis are hosts of this microorganism. The maximum enzymatic activities were obtained at 72 h (2.78 ± 0.015 IU/mL) and 96 h (3.44 ± 0.017 IU/mL), under 37 °C, pH = 4.5, and 70% humidity. At these conditions, the cellulolytic activity of the three enzymes was as follows: Endo-β (0.0547 ± 0.0167 IU/mL), Exo-β (0.0163 ± 0.001 IU/mL), and β-g (0.0692 ± 0.07 IU/mL). The data presented suggest that the isolated fungus has biotechnological potential as a source of cellulases, contributing to the field of little-studied phytopathogens, owing to the feasibility of biomass conversion. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of BioEnergy Research is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1007/s12155-025-10827-x Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 13 StartPage: 1 Subjects: – SubjectFull: Elongation factors (Biochemistry) Type: general – SubjectFull: Life sciences Type: general – SubjectFull: Biotechnology Type: general – SubjectFull: Identification of fungi Type: general – SubjectFull: Biomass conversion Type: general – SubjectFull: Solid-state fermentation Type: general Titles: – TitleFull: Isolation and Characterization of Neoscytalidium dimidiatum ITD-G6 from Agave durangensis Leaves for Enhanced Cellulase Production in Solid-State Cultivation. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Contreras-Hernández, M. G. – PersonEntity: Name: NameFull: Rutiaga-Quiñones, O. M. – PersonEntity: Name: NameFull: Mares-Rodríguez, F. J. – PersonEntity: Name: NameFull: Irigoyen-Campuzano, J. R. – PersonEntity: Name: NameFull: Cordero-Soto, I. N. – PersonEntity: Name: NameFull: Aréchiga-Carvajal, E. T. IsPartOfRelationships: – BibEntity: Dates: – D: 20 M: 02 Text: 2/20/2025 Type: published Y: 2025 Identifiers: – Type: issn-print Value: 19391234 Numbering: – Type: volume Value: 18 – Type: issue Value: 1 Titles: – TitleFull: BioEnergy Research Type: main |
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