Development of a multi-scale nanofiber scaffold platform for structurally and functionally replicated artificial perforating arteries.
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| Title: | Development of a multi-scale nanofiber scaffold platform for structurally and functionally replicated artificial perforating arteries. |
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| Authors: | Yoon, Su Jin1 (AUTHOR), Shin, Jae Ahn1 (AUTHOR), Shin, Hwa Sung1 (AUTHOR) hsshin@inha.ac.kr |
| Source: | Bioprocess & Biosystems Engineering. Mar2025, Vol. 48 Issue 3, p483-492. 10p. |
| Subjects: | Tissue culture, Tissue scaffolds, Ischemic stroke, Blood vessels, Tissue engineering |
| Abstract: | Experimental models for exploring abnormal brain blood vessels, including ischemic stroke, are crucial in neuroscience; recently, significant attention has been paid to artificial tissues through tissue engineering. Nanofibers, although commonly used as tissue engineering scaffolds, undergo structural deformations easily, making it challenging to create uniform tissue, especially for the smallest-diameter ones such as perforating arteries. This study focused on the development of a platform capable of reconstructing structurally and functionally replicated perforating arteries. To ensure structural consistency, 3D-printed modules were developed to minimize the structural deformation of nanofibrous scaffolds when integrated into a 3D-printed vessel culture dish. Surface structures and physical characteristics of the nanofibers before and after installation were compared using scanning electron microscopy, contact angle analysis, surface area analysis, and universal testing machine (UTM) analysis. The results showed a uniform thickness distribution, topography, maximum load, tensile strain, tensile strength, surface area, pore size, and pore volume of the nanofibers. For consistency in tissue culture, smooth muscle, endothelial, and astrocyte cells were co-cultured by continuously measuring the pH of the medium and replenishing the depleted glucose using the Kalman filter control system. The functional efficacy and consistency of the artificial perforating vessels were confirmed under oxidative stress induced by exposure to hydrogen peroxide. Transcriptional mRNA expression trends were similar to those in vivo for antioxidant enzymes, neurotrophic factors, inflammatory factors, and endothelial cell activation factors, with very low variation between tissues. This study provides a research platform for studying the oxidative stress environments related to stroke by mass-producing perforating arteries with consistent structures and functions. [ABSTRACT FROM AUTHOR] |
| Copyright of Bioprocess & Biosystems Engineering is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
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| Header | DbId: egs DbLabel: Engineering Source An: 183282496 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Development of a multi-scale nanofiber scaffold platform for structurally and functionally replicated artificial perforating arteries. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Yoon%2C+Su+Jin%22">Yoon, Su Jin</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Shin%2C+Jae+Ahn%22">Shin, Jae Ahn</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Shin%2C+Hwa+Sung%22">Shin, Hwa Sung</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> hsshin@inha.ac.kr</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Bioprocess+%26+Biosystems+Engineering%22">Bioprocess & Biosystems Engineering</searchLink>. Mar2025, Vol. 48 Issue 3, p483-492. 10p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Tissue+culture%22">Tissue culture</searchLink><br /><searchLink fieldCode="DE" term="%22Tissue+scaffolds%22">Tissue scaffolds</searchLink><br /><searchLink fieldCode="DE" term="%22Ischemic+stroke%22">Ischemic stroke</searchLink><br /><searchLink fieldCode="DE" term="%22Blood+vessels%22">Blood vessels</searchLink><br /><searchLink fieldCode="DE" term="%22Tissue+engineering%22">Tissue engineering</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Experimental models for exploring abnormal brain blood vessels, including ischemic stroke, are crucial in neuroscience; recently, significant attention has been paid to artificial tissues through tissue engineering. Nanofibers, although commonly used as tissue engineering scaffolds, undergo structural deformations easily, making it challenging to create uniform tissue, especially for the smallest-diameter ones such as perforating arteries. This study focused on the development of a platform capable of reconstructing structurally and functionally replicated perforating arteries. To ensure structural consistency, 3D-printed modules were developed to minimize the structural deformation of nanofibrous scaffolds when integrated into a 3D-printed vessel culture dish. Surface structures and physical characteristics of the nanofibers before and after installation were compared using scanning electron microscopy, contact angle analysis, surface area analysis, and universal testing machine (UTM) analysis. The results showed a uniform thickness distribution, topography, maximum load, tensile strain, tensile strength, surface area, pore size, and pore volume of the nanofibers. For consistency in tissue culture, smooth muscle, endothelial, and astrocyte cells were co-cultured by continuously measuring the pH of the medium and replenishing the depleted glucose using the Kalman filter control system. The functional efficacy and consistency of the artificial perforating vessels were confirmed under oxidative stress induced by exposure to hydrogen peroxide. Transcriptional mRNA expression trends were similar to those in vivo for antioxidant enzymes, neurotrophic factors, inflammatory factors, and endothelial cell activation factors, with very low variation between tissues. This study provides a research platform for studying the oxidative stress environments related to stroke by mass-producing perforating arteries with consistent structures and functions. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Bioprocess & Biosystems Engineering is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1007/s00449-024-03122-0 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 10 StartPage: 483 Subjects: – SubjectFull: Tissue culture Type: general – SubjectFull: Tissue scaffolds Type: general – SubjectFull: Ischemic stroke Type: general – SubjectFull: Blood vessels Type: general – SubjectFull: Tissue engineering Type: general Titles: – TitleFull: Development of a multi-scale nanofiber scaffold platform for structurally and functionally replicated artificial perforating arteries. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Yoon, Su Jin – PersonEntity: Name: NameFull: Shin, Jae Ahn – PersonEntity: Name: NameFull: Shin, Hwa Sung IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 03 Text: Mar2025 Type: published Y: 2025 Identifiers: – Type: issn-print Value: 16157591 Numbering: – Type: volume Value: 48 – Type: issue Value: 3 Titles: – TitleFull: Bioprocess & Biosystems Engineering Type: main |
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