View in EDS HTML Full Text PDF Full Text

Characterization of G‐quadruplexes in the Helicobacter pylori genome and assessment of therapeutic potential of G4 ligands.

Saved in:
Bibliographic Details
Title: Characterization of G‐quadruplexes in the Helicobacter pylori genome and assessment of therapeutic potential of G4 ligands.
Authors: Kumari, Monika1 (AUTHOR), Jaiswal, Saumya2 (AUTHOR), Shankar, Uma2 (AUTHOR), Gupta, Sharad2 (AUTHOR), Pradeepkumar, Pushpangadan Indira3 (AUTHOR), Kumar, Amit2 (AUTHOR), Nayak, Debasis4 (AUTHOR), Yadav, Vikas5 (AUTHOR) vikasjnu@gmail.com, Yadav, Puja1 (AUTHOR) pujayadav@cuh.ac.in
Source: Biotechnology & Applied Biochemistry. Feb2025, Vol. 72 Issue 1, p29-42. 14p.
Subjects: Nuclear magnetic resonance spectroscopy, Gene expression, Amino acid transport, Genetic regulation, Duodenal ulcers, Helicobacter pylori
Abstract: Helicobacter pylori, a leading human pathogen associated with duodenal ulcer and gastric cancer, presents a significant threat to human health due to increasing antibiotic resistance rates. This study investigates G‐quadruplexes (G4s), which are non‐canonical secondary structures form in G‐rich regions within the H. pylori genome. Extensive research on G4s in eukaryotes has revealed their role in epigenetically regulating cellular processes like gene transcription, DNA replication, and oncogene expression. However, understanding of G4‐mediated gene regulation in other organisms, especially bacterial pathogens, remains limited. Although G4 motifs have been extensively studied in a few bacterial species such as Mycobacterium, Streptococci, and Helicobacter, research on G4 motifs in other bacterial species is still sparse. Like in other organisms such as archaea, mammals, and viruses, G4s in H. pylori display a non‐random distribution primarily situated within open reading frames of various protein‐coding genes. The occurrence of G4s in functional regions of the genome and their conservation across different species indicates that their placement is not random, suggesting an evolutionary pressure to maintain these sequences at specific genomic sites. Moreover, G‐quadruplexes show enrichment in specific gene classes, suggesting their potential involvement in regulating the expression of genes related to cell wall/membrane/envelope biogenesis, amino acid transport, and metabolism. This indicates a probable regulatory role for G4s in controlling the expression of genes essential for H. pylori survival and virulence. Biophysical techniques such as Circular Dichroism spectroscopy and Nuclear Magnetic Resonance were used to characterize G4 motifs within selected H. pylori genes. The study revealed that G‐quadruplex ligand inhibited the growth of H. pylori, with minimal inhibitory concentrations in the low micromolar range. This suggests that targeting G4 structures could offer a promising approach for developing novel anti‐H. pylori drugs. [ABSTRACT FROM AUTHOR]
Copyright of Biotechnology & Applied Biochemistry is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
Full text is not displayed to guests.
FullText Links:
  – Type: pdflink
Text:
  Availability: 1
Header DbId: egs
DbLabel: Engineering Source
An: 183691374
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 0
IllustrationInfo
Items – Name: Title
  Label: Title
  Group: Ti
  Data: Characterization of G‐quadruplexes in the Helicobacter pylori genome and assessment of therapeutic potential of G4 ligands.
– Name: Author
  Label: Authors
  Group: Au
  Data: <searchLink fieldCode="AR" term="%22Kumari%2C+Monika%22">Kumari, Monika</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Jaiswal%2C+Saumya%22">Jaiswal, Saumya</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Shankar%2C+Uma%22">Shankar, Uma</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Gupta%2C+Sharad%22">Gupta, Sharad</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Pradeepkumar%2C+Pushpangadan+Indira%22">Pradeepkumar, Pushpangadan Indira</searchLink><relatesTo>3</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Kumar%2C+Amit%22">Kumar, Amit</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Nayak%2C+Debasis%22">Nayak, Debasis</searchLink><relatesTo>4</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Yadav%2C+Vikas%22">Yadav, Vikas</searchLink><relatesTo>5</relatesTo> (AUTHOR)<i> vikasjnu@gmail.com</i><br /><searchLink fieldCode="AR" term="%22Yadav%2C+Puja%22">Yadav, Puja</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> pujayadav@cuh.ac.in</i>
– Name: TitleSource
  Label: Source
  Group: Src
  Data: <searchLink fieldCode="JN" term="%22Biotechnology+%26+Applied+Biochemistry%22">Biotechnology & Applied Biochemistry</searchLink>. Feb2025, Vol. 72 Issue 1, p29-42. 14p.
– Name: Subject
  Label: Subjects
  Group: Su
  Data: <searchLink fieldCode="DE" term="%22Nuclear+magnetic+resonance+spectroscopy%22">Nuclear magnetic resonance spectroscopy</searchLink><br /><searchLink fieldCode="DE" term="%22Gene+expression%22">Gene expression</searchLink><br /><searchLink fieldCode="DE" term="%22Amino+acid+transport%22">Amino acid transport</searchLink><br /><searchLink fieldCode="DE" term="%22Genetic+regulation%22">Genetic regulation</searchLink><br /><searchLink fieldCode="DE" term="%22Duodenal+ulcers%22">Duodenal ulcers</searchLink><br /><searchLink fieldCode="DE" term="%22Helicobacter+pylori%22">Helicobacter pylori</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Helicobacter pylori, a leading human pathogen associated with duodenal ulcer and gastric cancer, presents a significant threat to human health due to increasing antibiotic resistance rates. This study investigates G‐quadruplexes (G4s), which are non‐canonical secondary structures form in G‐rich regions within the H. pylori genome. Extensive research on G4s in eukaryotes has revealed their role in epigenetically regulating cellular processes like gene transcription, DNA replication, and oncogene expression. However, understanding of G4‐mediated gene regulation in other organisms, especially bacterial pathogens, remains limited. Although G4 motifs have been extensively studied in a few bacterial species such as Mycobacterium, Streptococci, and Helicobacter, research on G4 motifs in other bacterial species is still sparse. Like in other organisms such as archaea, mammals, and viruses, G4s in H. pylori display a non‐random distribution primarily situated within open reading frames of various protein‐coding genes. The occurrence of G4s in functional regions of the genome and their conservation across different species indicates that their placement is not random, suggesting an evolutionary pressure to maintain these sequences at specific genomic sites. Moreover, G‐quadruplexes show enrichment in specific gene classes, suggesting their potential involvement in regulating the expression of genes related to cell wall/membrane/envelope biogenesis, amino acid transport, and metabolism. This indicates a probable regulatory role for G4s in controlling the expression of genes essential for H. pylori survival and virulence. Biophysical techniques such as Circular Dichroism spectroscopy and Nuclear Magnetic Resonance were used to characterize G4 motifs within selected H. pylori genes. The study revealed that G‐quadruplex ligand inhibited the growth of H. pylori, with minimal inhibitory concentrations in the low micromolar range. This suggests that targeting G4 structures could offer a promising approach for developing novel anti‐H. pylori drugs. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Biotechnology & Applied Biochemistry is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
PLink https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=egs&AN=183691374
RecordInfo BibRecord:
  BibEntity:
    Identifiers:
      – Type: doi
        Value: 10.1002/bab.2644
    Languages:
      – Code: eng
        Text: English
    PhysicalDescription:
      Pagination:
        PageCount: 14
        StartPage: 29
    Subjects:
      – SubjectFull: Nuclear magnetic resonance spectroscopy
        Type: general
      – SubjectFull: Gene expression
        Type: general
      – SubjectFull: Amino acid transport
        Type: general
      – SubjectFull: Genetic regulation
        Type: general
      – SubjectFull: Duodenal ulcers
        Type: general
      – SubjectFull: Helicobacter pylori
        Type: general
    Titles:
      – TitleFull: Characterization of G‐quadruplexes in the Helicobacter pylori genome and assessment of therapeutic potential of G4 ligands.
        Type: main
  BibRelationships:
    HasContributorRelationships:
      – PersonEntity:
          Name:
            NameFull: Kumari, Monika
      – PersonEntity:
          Name:
            NameFull: Jaiswal, Saumya
      – PersonEntity:
          Name:
            NameFull: Shankar, Uma
      – PersonEntity:
          Name:
            NameFull: Gupta, Sharad
      – PersonEntity:
          Name:
            NameFull: Pradeepkumar, Pushpangadan Indira
      – PersonEntity:
          Name:
            NameFull: Kumar, Amit
      – PersonEntity:
          Name:
            NameFull: Nayak, Debasis
      – PersonEntity:
          Name:
            NameFull: Yadav, Vikas
      – PersonEntity:
          Name:
            NameFull: Yadav, Puja
    IsPartOfRelationships:
      – BibEntity:
          Dates:
            – D: 01
              M: 02
              Text: Feb2025
              Type: published
              Y: 2025
          Identifiers:
            – Type: issn-print
              Value: 08854513
          Numbering:
            – Type: volume
              Value: 72
            – Type: issue
              Value: 1
          Titles:
            – TitleFull: Biotechnology & Applied Biochemistry
              Type: main
ResultId 1