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Cow's Milk Protein May Induce Allergy and Inflammation in Intestinal Epithelial Cells Through Regulating HOTAIR Expression and NF‐κB Signaling Pathway.

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Title: Cow's Milk Protein May Induce Allergy and Inflammation in Intestinal Epithelial Cells Through Regulating HOTAIR Expression and NF‐κB Signaling Pathway.
Authors: Mao, Kangwei1 (AUTHOR), Liu, Jiangyan1 (AUTHOR), Li, Dalei1 (AUTHOR), Gu, Siyu1 (AUTHOR), Zhang, Chenxi1 (AUTHOR), Li, Mengmeng2 (AUTHOR), Sun, Jun1 (AUTHOR) lygsunjun@126.com, Wang, Juan1 (AUTHOR) wangjuan19820206@163.com, Manna, Prasenjit (AUTHOR)
Source: Journal of Food Biochemistry. 1/23/2025, Vol. 2025, p1-9. 9p.
Subjects: Milk allergy, Milk proteins, LincRNA, Inflammatory mediators, Antisense RNA
Abstract: Background: Cow's milk protein allergy (CMPA) is associated with activation of proinflammatory signaling pathways and overexpression of inflammatory mediators. Long noncoding RNA (LncRNA) HOX transcript antisense intergenic RNA (HOTAIR) is a LncRNA, which is involved in the occurrence and development of many biological processes and diseases. HOTAIR can prevent necrotizing enterocolitis. This study aims to explore the effect of milk protein on NCM‐460 cells and its mechanism of action with HOTAIR. Methods: NCM‐460 cells were induced by cow's milk protein to establish in vitro cell models. CCK‐8 and EdU staining were used for evaluating the effects of cow's milk protein on the viability and proliferation. ELISA was used for comparing the levels of inflammatory cytokines. TUNEL staining was conducted for evaluating the apoptosis. Expression levels of HOTAIR were detected by RT‐qPCR. The expression of NF‐κB signaling pathway–related molecules in cells was explored to evaluate the mechanism of HOTAIR in improving ALI. Results: Cow's milk protein decreased the viability NCM‐460 cells and also decreased the expression of HOTAIR. Moreover, it can induce the antiproliferative and proapoptotic effects on NCM‐460 cells, and overexpression of LncRNA HOTAIR can partially reverse the effects of cow's milk protein. In addition, overexpression of LncRNA HOTAIR reversed the effects of cow's milk protein on NF‐κB signaling. Conclusions: Cow's milk protein may induce the allergy and inflammation in intestinal epithelial cells through regulating HOTAIR expression and NF‐κB signaling pathways. [ABSTRACT FROM AUTHOR]
Copyright of Journal of Food Biochemistry is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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PubTypeId: academicJournal
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Items – Name: Title
  Label: Title
  Group: Ti
  Data: Cow's Milk Protein May Induce Allergy and Inflammation in Intestinal Epithelial Cells Through Regulating HOTAIR Expression and NF‐κB Signaling Pathway.
– Name: Author
  Label: Authors
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  Data: <searchLink fieldCode="AR" term="%22Mao%2C+Kangwei%22">Mao, Kangwei</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Liu%2C+Jiangyan%22">Liu, Jiangyan</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Li%2C+Dalei%22">Li, Dalei</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Gu%2C+Siyu%22">Gu, Siyu</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Zhang%2C+Chenxi%22">Zhang, Chenxi</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Li%2C+Mengmeng%22">Li, Mengmeng</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Sun%2C+Jun%22">Sun, Jun</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> lygsunjun@126.com</i><br /><searchLink fieldCode="AR" term="%22Wang%2C+Juan%22">Wang, Juan</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> wangjuan19820206@163.com</i><br /><searchLink fieldCode="AR" term="%22Manna%2C+Prasenjit%22">Manna, Prasenjit</searchLink> (AUTHOR)
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  Data: <searchLink fieldCode="JN" term="%22Journal+of+Food+Biochemistry%22">Journal of Food Biochemistry</searchLink>. 1/23/2025, Vol. 2025, p1-9. 9p.
– Name: Subject
  Label: Subjects
  Group: Su
  Data: <searchLink fieldCode="DE" term="%22Milk+allergy%22">Milk allergy</searchLink><br /><searchLink fieldCode="DE" term="%22Milk+proteins%22">Milk proteins</searchLink><br /><searchLink fieldCode="DE" term="%22LincRNA%22">LincRNA</searchLink><br /><searchLink fieldCode="DE" term="%22Inflammatory+mediators%22">Inflammatory mediators</searchLink><br /><searchLink fieldCode="DE" term="%22Antisense+RNA%22">Antisense RNA</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Background: Cow's milk protein allergy (CMPA) is associated with activation of proinflammatory signaling pathways and overexpression of inflammatory mediators. Long noncoding RNA (LncRNA) HOX transcript antisense intergenic RNA (HOTAIR) is a LncRNA, which is involved in the occurrence and development of many biological processes and diseases. HOTAIR can prevent necrotizing enterocolitis. This study aims to explore the effect of milk protein on NCM‐460 cells and its mechanism of action with HOTAIR. Methods: NCM‐460 cells were induced by cow's milk protein to establish in vitro cell models. CCK‐8 and EdU staining were used for evaluating the effects of cow's milk protein on the viability and proliferation. ELISA was used for comparing the levels of inflammatory cytokines. TUNEL staining was conducted for evaluating the apoptosis. Expression levels of HOTAIR were detected by RT‐qPCR. The expression of NF‐κB signaling pathway–related molecules in cells was explored to evaluate the mechanism of HOTAIR in improving ALI. Results: Cow's milk protein decreased the viability NCM‐460 cells and also decreased the expression of HOTAIR. Moreover, it can induce the antiproliferative and proapoptotic effects on NCM‐460 cells, and overexpression of LncRNA HOTAIR can partially reverse the effects of cow's milk protein. In addition, overexpression of LncRNA HOTAIR reversed the effects of cow's milk protein on NF‐κB signaling. Conclusions: Cow's milk protein may induce the allergy and inflammation in intestinal epithelial cells through regulating HOTAIR expression and NF‐κB signaling pathways. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Journal of Food Biochemistry is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Value: 10.1155/jfbc/8448913
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      – Code: eng
        Text: English
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        PageCount: 9
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    Subjects:
      – SubjectFull: Milk allergy
        Type: general
      – SubjectFull: Milk proteins
        Type: general
      – SubjectFull: LincRNA
        Type: general
      – SubjectFull: Inflammatory mediators
        Type: general
      – SubjectFull: Antisense RNA
        Type: general
    Titles:
      – TitleFull: Cow's Milk Protein May Induce Allergy and Inflammation in Intestinal Epithelial Cells Through Regulating HOTAIR Expression and NF‐κB Signaling Pathway.
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            NameFull: Mao, Kangwei
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            – D: 23
              M: 01
              Text: 1/23/2025
              Type: published
              Y: 2025
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