View in EDS HTML Full Text PDF Full Text

Improved Neisseria gonorrhoeae culture media without atmospheric CO2.

Saved in:
Bibliographic Details
Title: Improved Neisseria gonorrhoeae culture media without atmospheric CO2.
Authors: Menkiti, Chukwuma Jude1 (AUTHOR), Snyder, Lori A. S.1 (AUTHOR) L.Snyder@kingston.ac.uk
Source: Applied Microbiology & Biotechnology. 3/22/2025, Vol. 109 Issue 1, p1-8. 8p.
Subjects: COVID-19 pandemic, Neisseria gonorrhoeae, Iron supplements, Sodium bicarbonate, Bacterial cultures
Abstract: Bacterial culture on solid media is the crucial step in diagnosing Neisseria gonorrhoeae infections and is the gold standard for determining their antimicrobial resistance profile. However, culture of Neisseria spp. can be challenging in resource poor areas, relying on specialist incubators or other methods of supplying 5% CO2 for growth of the bacteria. Even when such incubators are available, the CO2 to run them may be scarce; there were CO2 shortages during the COVID-19 pandemic, for example. Although culture jars with gas packs or candles can be used, these are inefficient in terms of use of incubator space and researcher time. To achieve simplicity in culturing of N. gonorrhoeae, the standard Oxoid GC agar base medium, made with the Kellogg's glucose and iron supplements was improved with the addition of 0.75 g/l sodium bicarbonate (NaHCO3), which is inexpensive and readily available. This improved media in a standard incubator performed as well as standard Oxoid GC agar media with supplements in a 5% CO2 incubator. Chocolate agar and Thayer-Martin agar with sodium bicarbonate were also developed, with all showing good growth of N. gonorrhoeae without the need for atmospheric CO2. Key points: • Neisseria spp. (N. gonorrhoeae, N. meningitidis) require atmospheric CO2 to grow. • Sources of CO2 may be scarce depending on geography and lab supply availability. • We have developed GC, Chocolate, and Thayer-Martin media that does not need CO2. [ABSTRACT FROM AUTHOR]
Copyright of Applied Microbiology & Biotechnology is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
Full text is not displayed to guests.
FullText Links:
  – Type: pdflink
Text:
  Availability: 1
Header DbId: egs
DbLabel: Engineering Source
An: 183974179
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 0
IllustrationInfo
Items – Name: Title
  Label: Title
  Group: Ti
  Data: Improved Neisseria gonorrhoeae culture media without atmospheric CO<subscript>2</subscript>.
– Name: Author
  Label: Authors
  Group: Au
  Data: <searchLink fieldCode="AR" term="%22Menkiti%2C+Chukwuma+Jude%22">Menkiti, Chukwuma Jude</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Snyder%2C+Lori+A%2E+S%2E%22">Snyder, Lori A. S.</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> L.Snyder@kingston.ac.uk</i>
– Name: TitleSource
  Label: Source
  Group: Src
  Data: <searchLink fieldCode="JN" term="%22Applied+Microbiology+%26+Biotechnology%22">Applied Microbiology & Biotechnology</searchLink>. 3/22/2025, Vol. 109 Issue 1, p1-8. 8p.
– Name: Subject
  Label: Subjects
  Group: Su
  Data: <searchLink fieldCode="DE" term="%22COVID-19+pandemic%22">COVID-19 pandemic</searchLink><br /><searchLink fieldCode="DE" term="%22Neisseria+gonorrhoeae%22">Neisseria gonorrhoeae</searchLink><br /><searchLink fieldCode="DE" term="%22Iron+supplements%22">Iron supplements</searchLink><br /><searchLink fieldCode="DE" term="%22Sodium+bicarbonate%22">Sodium bicarbonate</searchLink><br /><searchLink fieldCode="DE" term="%22Bacterial+cultures%22">Bacterial cultures</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Bacterial culture on solid media is the crucial step in diagnosing Neisseria gonorrhoeae infections and is the gold standard for determining their antimicrobial resistance profile. However, culture of Neisseria spp. can be challenging in resource poor areas, relying on specialist incubators or other methods of supplying 5% CO2 for growth of the bacteria. Even when such incubators are available, the CO2 to run them may be scarce; there were CO2 shortages during the COVID-19 pandemic, for example. Although culture jars with gas packs or candles can be used, these are inefficient in terms of use of incubator space and researcher time. To achieve simplicity in culturing of N. gonorrhoeae, the standard Oxoid GC agar base medium, made with the Kellogg's glucose and iron supplements was improved with the addition of 0.75 g/l sodium bicarbonate (NaHCO3), which is inexpensive and readily available. This improved media in a standard incubator performed as well as standard Oxoid GC agar media with supplements in a 5% CO2 incubator. Chocolate agar and Thayer-Martin agar with sodium bicarbonate were also developed, with all showing good growth of N. gonorrhoeae without the need for atmospheric CO2. Key points: • Neisseria spp. (N. gonorrhoeae, N. meningitidis) require atmospheric CO2 to grow. • Sources of CO2 may be scarce depending on geography and lab supply availability. • We have developed GC, Chocolate, and Thayer-Martin media that does not need CO2. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Applied Microbiology & Biotechnology is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
PLink https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=egs&AN=183974179
RecordInfo BibRecord:
  BibEntity:
    Identifiers:
      – Type: doi
        Value: 10.1007/s00253-025-13449-7
    Languages:
      – Code: eng
        Text: English
    PhysicalDescription:
      Pagination:
        PageCount: 8
        StartPage: 1
    Subjects:
      – SubjectFull: COVID-19 pandemic
        Type: general
      – SubjectFull: Neisseria gonorrhoeae
        Type: general
      – SubjectFull: Iron supplements
        Type: general
      – SubjectFull: Sodium bicarbonate
        Type: general
      – SubjectFull: Bacterial cultures
        Type: general
    Titles:
      – TitleFull: Improved Neisseria gonorrhoeae culture media without atmospheric CO2.
        Type: main
  BibRelationships:
    HasContributorRelationships:
      – PersonEntity:
          Name:
            NameFull: Menkiti, Chukwuma Jude
      – PersonEntity:
          Name:
            NameFull: Snyder, Lori A. S.
    IsPartOfRelationships:
      – BibEntity:
          Dates:
            – D: 22
              M: 03
              Text: 3/22/2025
              Type: published
              Y: 2025
          Identifiers:
            – Type: issn-print
              Value: 01757598
          Numbering:
            – Type: volume
              Value: 109
            – Type: issue
              Value: 1
          Titles:
            – TitleFull: Applied Microbiology & Biotechnology
              Type: main
ResultId 1