View in EDS HTML Full Text PDF Full Text

Creating a gene-indexed EMS mutation library of Zheng58 for improving maize genetics research.

Saved in:
Bibliographic Details
Title: Creating a gene-indexed EMS mutation library of Zheng58 for improving maize genetics research.
Authors: Chong, Leelyn1 (AUTHOR), Su, Huihui1 (AUTHOR), Liu, Yingpeng1 (AUTHOR), Zheng, Lingling1 (AUTHOR), Tao, Lianying1 (AUTHOR), Bie, Hai2 (AUTHOR), Xiao, Di1 (AUTHOR), Yang, Yang1 (AUTHOR), Zhang, Wanjun1 (AUTHOR), Zhang, Jing1 (AUTHOR), Ren, Jifei1 (AUTHOR), Liu, Huafeng1 (AUTHOR), Ren, Zhenzhen1 (AUTHOR), Chen, Yanhui1 (AUTHOR), Xi, Zhangying1 (AUTHOR) xizhangying@henau.edu.cn, Li, Chengwei1 (AUTHOR) lcw@henau.edu.cn, Ku, Lixia1 (AUTHOR) kulixia0371@henau.edu.cn
Source: Theoretical & Applied Genetics. Apr2025, Vol. 138 Issue 4, p1-10. 10p.
Abstract: Key message: We created the Zheng58 EMS Mutation Library, which provides a valuable resource for future investigations into the functions and roles of specific genes in maize physiology and development. Understanding the genetic variations present within maize is fundamental to improving maize breeding programs and developing crops with desirable traits. In this work, the Gene-Indexed Ethyl Methanesulfonate (EMS) Mutation Library of Zheng58 in maize was created to accelerate maize genetics research. By chemically inducing mutations in the Zheng58 maize inbred line using (EMS), 426 M2 EMS lines were generated, of which 185 exhibited heritable phenotypic changes. Coupling with high throughput sequencing techniques, over two million mutations, encompassing single nucleotide polymorphisms (SNPs) and small insertions and deletions (InDels), were subsequently identified. Functional annotation of mutation sites further indicated that a significant number of mutations influences important cellular processes, including translation termination (8,279), splice site disruption leading to mis-splicing (9,504), and missense mutations affecting protein-coding sequences (52,494). Therefore, the Gene-Indexed EMS Mutation Library of maize Zheng58 provides a valuable resource for future investigations into the functions and roles of specific genes in maize physiology and development. [ABSTRACT FROM AUTHOR]
Copyright of Theoretical & Applied Genetics is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
Full text is not displayed to guests.
FullText Links:
  – Type: pdflink
Text:
  Availability: 1
Header DbId: egs
DbLabel: Engineering Source
An: 184114899
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 0
IllustrationInfo
Items – Name: Title
  Label: Title
  Group: Ti
  Data: Creating a gene-indexed EMS mutation library of Zheng58 for improving maize genetics research.
– Name: Author
  Label: Authors
  Group: Au
  Data: <searchLink fieldCode="AR" term="%22Chong%2C+Leelyn%22">Chong, Leelyn</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Su%2C+Huihui%22">Su, Huihui</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Liu%2C+Yingpeng%22">Liu, Yingpeng</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Zheng%2C+Lingling%22">Zheng, Lingling</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Tao%2C+Lianying%22">Tao, Lianying</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Bie%2C+Hai%22">Bie, Hai</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Xiao%2C+Di%22">Xiao, Di</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Yang%2C+Yang%22">Yang, Yang</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Zhang%2C+Wanjun%22">Zhang, Wanjun</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Zhang%2C+Jing%22">Zhang, Jing</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Ren%2C+Jifei%22">Ren, Jifei</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Liu%2C+Huafeng%22">Liu, Huafeng</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Ren%2C+Zhenzhen%22">Ren, Zhenzhen</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Chen%2C+Yanhui%22">Chen, Yanhui</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Xi%2C+Zhangying%22">Xi, Zhangying</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> xizhangying@henau.edu.cn</i><br /><searchLink fieldCode="AR" term="%22Li%2C+Chengwei%22">Li, Chengwei</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> lcw@henau.edu.cn</i><br /><searchLink fieldCode="AR" term="%22Ku%2C+Lixia%22">Ku, Lixia</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> kulixia0371@henau.edu.cn</i>
– Name: TitleSource
  Label: Source
  Group: Src
  Data: <searchLink fieldCode="JN" term="%22Theoretical+%26+Applied+Genetics%22">Theoretical & Applied Genetics</searchLink>. Apr2025, Vol. 138 Issue 4, p1-10. 10p.
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Key message: We created the Zheng58 EMS Mutation Library, which provides a valuable resource for future investigations into the functions and roles of specific genes in maize physiology and development. Understanding the genetic variations present within maize is fundamental to improving maize breeding programs and developing crops with desirable traits. In this work, the Gene-Indexed Ethyl Methanesulfonate (EMS) Mutation Library of Zheng58 in maize was created to accelerate maize genetics research. By chemically inducing mutations in the Zheng58 maize inbred line using (EMS), 426 M2 EMS lines were generated, of which 185 exhibited heritable phenotypic changes. Coupling with high throughput sequencing techniques, over two million mutations, encompassing single nucleotide polymorphisms (SNPs) and small insertions and deletions (InDels), were subsequently identified. Functional annotation of mutation sites further indicated that a significant number of mutations influences important cellular processes, including translation termination (8,279), splice site disruption leading to mis-splicing (9,504), and missense mutations affecting protein-coding sequences (52,494). Therefore, the Gene-Indexed EMS Mutation Library of maize Zheng58 provides a valuable resource for future investigations into the functions and roles of specific genes in maize physiology and development. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Theoretical & Applied Genetics is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
PLink https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=egs&AN=184114899
RecordInfo BibRecord:
  BibEntity:
    Identifiers:
      – Type: doi
        Value: 10.1007/s00122-025-04878-z
    Languages:
      – Code: eng
        Text: English
    PhysicalDescription:
      Pagination:
        PageCount: 10
        StartPage: 1
    Titles:
      – TitleFull: Creating a gene-indexed EMS mutation library of Zheng58 for improving maize genetics research.
        Type: main
  BibRelationships:
    HasContributorRelationships:
      – PersonEntity:
          Name:
            NameFull: Chong, Leelyn
      – PersonEntity:
          Name:
            NameFull: Su, Huihui
      – PersonEntity:
          Name:
            NameFull: Liu, Yingpeng
      – PersonEntity:
          Name:
            NameFull: Zheng, Lingling
      – PersonEntity:
          Name:
            NameFull: Tao, Lianying
      – PersonEntity:
          Name:
            NameFull: Bie, Hai
      – PersonEntity:
          Name:
            NameFull: Xiao, Di
      – PersonEntity:
          Name:
            NameFull: Yang, Yang
      – PersonEntity:
          Name:
            NameFull: Zhang, Wanjun
      – PersonEntity:
          Name:
            NameFull: Zhang, Jing
      – PersonEntity:
          Name:
            NameFull: Ren, Jifei
      – PersonEntity:
          Name:
            NameFull: Liu, Huafeng
      – PersonEntity:
          Name:
            NameFull: Ren, Zhenzhen
      – PersonEntity:
          Name:
            NameFull: Chen, Yanhui
      – PersonEntity:
          Name:
            NameFull: Xi, Zhangying
      – PersonEntity:
          Name:
            NameFull: Li, Chengwei
      – PersonEntity:
          Name:
            NameFull: Ku, Lixia
    IsPartOfRelationships:
      – BibEntity:
          Dates:
            – D: 01
              M: 04
              Text: Apr2025
              Type: published
              Y: 2025
          Identifiers:
            – Type: issn-print
              Value: 00405752
          Numbering:
            – Type: volume
              Value: 138
            – Type: issue
              Value: 4
          Titles:
            – TitleFull: Theoretical & Applied Genetics
              Type: main
ResultId 1