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Targeted inhibition of oral biofilm formation using phage-derived high-affinity peptides.

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Title: Targeted inhibition of oral biofilm formation using phage-derived high-affinity peptides.
Authors: Lee, Jaewoong1,2 (AUTHOR), Shin, Woo-Ri1,3 (AUTHOR), Kim, Yang-Hoon1,4,5 (AUTHOR) kyh@chungbuk.ac.kr, Ahn, Ji-Young1,4 (AUTHOR) jyahn@chungbuk.ac.kr, Chae, Soryong1,6 (AUTHOR) chaesg@ucmail.uc.edu, Min, Jiho1 (AUTHOR) jihomin@jbnu.ac.kr
Source: Journal of Biotechnology. Jun2025, Vol. 402, p51-58. 8p.
Subjects: Dental caries, Oral diseases, Age groups, Bacteriophages, Peptides
Abstract: Dental caries, commonly known as tooth decay, poses a significant oral health challenge affecting individuals of all age groups. While dietary factors play a role, tooth decay primarily results from the activity of various oral bacteria that form biofilms in the oral cavity. In this study, we employed the phage display technique to identify high-affinity peptides capable of binding specifically to three oral bacteria strains: Streptococcus mutans , Streptococcus oralis , and Lactobacillus casei. Four selected peptides underwent binding affinity testing for each target bacterium, revealing that three of them exhibited specific binding capabilities, effectively inhibiting biofilm formation. This study demonstrates the efficacy of engineered phages in identifying high-affinity peptides that selectively target oral bacteria. These peptides hold promise for preventing oral biofilm formation, a significant contributor to oral diseases and dental caries. This innovative approach opens doors to novel therapeutic strategies for addressing oral health issues. The findings may spur further research into the utilization of phages and peptides as potential anti-biofilm agents, potentially revolutionizing the field of oral health. [Display omitted] • The target non-immobilization method was used for effective phage selection. • Sequences (SmP, SoP and LcP2) were obtained for S.mutans , S.oralis and L.casei. • SmP, SoP, and LcP2 showed antibacterial activity compared to each target. • The peptides could significantly inhibit the biofilm formation of oral bacteria. [ABSTRACT FROM AUTHOR]
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Database: Engineering Source
Description
Abstract:Dental caries, commonly known as tooth decay, poses a significant oral health challenge affecting individuals of all age groups. While dietary factors play a role, tooth decay primarily results from the activity of various oral bacteria that form biofilms in the oral cavity. In this study, we employed the phage display technique to identify high-affinity peptides capable of binding specifically to three oral bacteria strains: Streptococcus mutans , Streptococcus oralis , and Lactobacillus casei. Four selected peptides underwent binding affinity testing for each target bacterium, revealing that three of them exhibited specific binding capabilities, effectively inhibiting biofilm formation. This study demonstrates the efficacy of engineered phages in identifying high-affinity peptides that selectively target oral bacteria. These peptides hold promise for preventing oral biofilm formation, a significant contributor to oral diseases and dental caries. This innovative approach opens doors to novel therapeutic strategies for addressing oral health issues. The findings may spur further research into the utilization of phages and peptides as potential anti-biofilm agents, potentially revolutionizing the field of oral health. [Display omitted] • The target non-immobilization method was used for effective phage selection. • Sequences (SmP, SoP and LcP2) were obtained for S.mutans , S.oralis and L.casei. • SmP, SoP, and LcP2 showed antibacterial activity compared to each target. • The peptides could significantly inhibit the biofilm formation of oral bacteria. [ABSTRACT FROM AUTHOR]
ISSN:01681656
DOI:10.1016/j.jbiotec.2025.03.009