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Targeted inhibition of oral biofilm formation using phage-derived high-affinity peptides.

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Title: Targeted inhibition of oral biofilm formation using phage-derived high-affinity peptides.
Authors: Lee, Jaewoong1,2 (AUTHOR), Shin, Woo-Ri1,3 (AUTHOR), Kim, Yang-Hoon1,4,5 (AUTHOR) kyh@chungbuk.ac.kr, Ahn, Ji-Young1,4 (AUTHOR) jyahn@chungbuk.ac.kr, Chae, Soryong1,6 (AUTHOR) chaesg@ucmail.uc.edu, Min, Jiho1 (AUTHOR) jihomin@jbnu.ac.kr
Source: Journal of Biotechnology. Jun2025, Vol. 402, p51-58. 8p.
Subjects: Dental caries, Oral diseases, Age groups, Bacteriophages, Peptides
Abstract: Dental caries, commonly known as tooth decay, poses a significant oral health challenge affecting individuals of all age groups. While dietary factors play a role, tooth decay primarily results from the activity of various oral bacteria that form biofilms in the oral cavity. In this study, we employed the phage display technique to identify high-affinity peptides capable of binding specifically to three oral bacteria strains: Streptococcus mutans , Streptococcus oralis , and Lactobacillus casei. Four selected peptides underwent binding affinity testing for each target bacterium, revealing that three of them exhibited specific binding capabilities, effectively inhibiting biofilm formation. This study demonstrates the efficacy of engineered phages in identifying high-affinity peptides that selectively target oral bacteria. These peptides hold promise for preventing oral biofilm formation, a significant contributor to oral diseases and dental caries. This innovative approach opens doors to novel therapeutic strategies for addressing oral health issues. The findings may spur further research into the utilization of phages and peptides as potential anti-biofilm agents, potentially revolutionizing the field of oral health. [Display omitted] • The target non-immobilization method was used for effective phage selection. • Sequences (SmP, SoP and LcP2) were obtained for S.mutans , S.oralis and L.casei. • SmP, SoP, and LcP2 showed antibacterial activity compared to each target. • The peptides could significantly inhibit the biofilm formation of oral bacteria. [ABSTRACT FROM AUTHOR]
Copyright of Journal of Biotechnology is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
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Header DbId: egs
DbLabel: Engineering Source
An: 184264938
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 0
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Items – Name: Title
  Label: Title
  Group: Ti
  Data: Targeted inhibition of oral biofilm formation using phage-derived high-affinity peptides.
– Name: Author
  Label: Authors
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  Data: <searchLink fieldCode="AR" term="%22Lee%2C+Jaewoong%22">Lee, Jaewoong</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Shin%2C+Woo-Ri%22">Shin, Woo-Ri</searchLink><relatesTo>1,3</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Kim%2C+Yang-Hoon%22">Kim, Yang-Hoon</searchLink><relatesTo>1,4,5</relatesTo> (AUTHOR)<i> kyh@chungbuk.ac.kr</i><br /><searchLink fieldCode="AR" term="%22Ahn%2C+Ji-Young%22">Ahn, Ji-Young</searchLink><relatesTo>1,4</relatesTo> (AUTHOR)<i> jyahn@chungbuk.ac.kr</i><br /><searchLink fieldCode="AR" term="%22Chae%2C+Soryong%22">Chae, Soryong</searchLink><relatesTo>1,6</relatesTo> (AUTHOR)<i> chaesg@ucmail.uc.edu</i><br /><searchLink fieldCode="AR" term="%22Min%2C+Jiho%22">Min, Jiho</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> jihomin@jbnu.ac.kr</i>
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  Data: <searchLink fieldCode="JN" term="%22Journal+of+Biotechnology%22">Journal of Biotechnology</searchLink>. Jun2025, Vol. 402, p51-58. 8p.
– Name: Subject
  Label: Subjects
  Group: Su
  Data: <searchLink fieldCode="DE" term="%22Dental+caries%22">Dental caries</searchLink><br /><searchLink fieldCode="DE" term="%22Oral+diseases%22">Oral diseases</searchLink><br /><searchLink fieldCode="DE" term="%22Age+groups%22">Age groups</searchLink><br /><searchLink fieldCode="DE" term="%22Bacteriophages%22">Bacteriophages</searchLink><br /><searchLink fieldCode="DE" term="%22Peptides%22">Peptides</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Dental caries, commonly known as tooth decay, poses a significant oral health challenge affecting individuals of all age groups. While dietary factors play a role, tooth decay primarily results from the activity of various oral bacteria that form biofilms in the oral cavity. In this study, we employed the phage display technique to identify high-affinity peptides capable of binding specifically to three oral bacteria strains: Streptococcus mutans , Streptococcus oralis , and Lactobacillus casei. Four selected peptides underwent binding affinity testing for each target bacterium, revealing that three of them exhibited specific binding capabilities, effectively inhibiting biofilm formation. This study demonstrates the efficacy of engineered phages in identifying high-affinity peptides that selectively target oral bacteria. These peptides hold promise for preventing oral biofilm formation, a significant contributor to oral diseases and dental caries. This innovative approach opens doors to novel therapeutic strategies for addressing oral health issues. The findings may spur further research into the utilization of phages and peptides as potential anti-biofilm agents, potentially revolutionizing the field of oral health. [Display omitted] • The target non-immobilization method was used for effective phage selection. • Sequences (SmP, SoP and LcP2) were obtained for S.mutans , S.oralis and L.casei. • SmP, SoP, and LcP2 showed antibacterial activity compared to each target. • The peptides could significantly inhibit the biofilm formation of oral bacteria. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Journal of Biotechnology is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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RecordInfo BibRecord:
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      – Type: doi
        Value: 10.1016/j.jbiotec.2025.03.009
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      – Code: eng
        Text: English
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      Pagination:
        PageCount: 8
        StartPage: 51
    Subjects:
      – SubjectFull: Dental caries
        Type: general
      – SubjectFull: Oral diseases
        Type: general
      – SubjectFull: Age groups
        Type: general
      – SubjectFull: Bacteriophages
        Type: general
      – SubjectFull: Peptides
        Type: general
    Titles:
      – TitleFull: Targeted inhibition of oral biofilm formation using phage-derived high-affinity peptides.
        Type: main
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          Name:
            NameFull: Lee, Jaewoong
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            NameFull: Shin, Woo-Ri
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            NameFull: Kim, Yang-Hoon
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            NameFull: Ahn, Ji-Young
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            NameFull: Chae, Soryong
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            NameFull: Min, Jiho
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            – D: 01
              M: 06
              Text: Jun2025
              Type: published
              Y: 2025
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              Value: 01681656
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              Value: 402
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            – TitleFull: Journal of Biotechnology
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