Annexin A1 protects periodontal ligament cells against lipopolysaccharide‐induced inflammatory response and cellular senescence: An implication in periodontitis.
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| Title: | Annexin A1 protects periodontal ligament cells against lipopolysaccharide‐induced inflammatory response and cellular senescence: An implication in periodontitis. |
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| Authors: | Luo, Shuwen1 (AUTHOR), Zhang, Lin1 (AUTHOR), Li, Xiaoyu1 (AUTHOR), Tong, Chunshi1 (AUTHOR) tongchuns003@126.com |
| Source: | Biotechnology & Applied Biochemistry. Apr2025, Vol. 72 Issue 2, p449-459. 11p. |
| Subjects: | Telomerase reverse transcriptase, Cellular aging, Sirtuins, Annexins, Periodontal ligament |
| Abstract: | Periodontitis is an inflammatory condition that affects the tooth‐supporting structures, triggered by the host's immune response toward the bacterial deposits around the teeth. Annexin A1 (AnxA1), a vital member of the annexin superfamily, is known for its diverse physiological functions, particularly its anti‐inflammatory and anti‐senescence properties. We hypothesized that AnxA1 has a protective effect against lipopolysaccharide (LPS)‐induced inflammatory responses and cellular damage in periodontal ligament cells (PDLCs). In this study, we demonstrate that LPS stimulation significantly reduced telomerase activity in PDLCs, a decline that was dose‐dependently reversed by AnxA1. Importantly, AnxA1 protected the cells from LPS‐induced cellular senescence and the downregulation of human telomerase reverse transcriptase (hTERT) expression. In line with this, AnxA1 suppressed the LPS‐induced expression of p21 and p16 at both the mRNA and protein levels. Furthermore, AnxA1 demonstrated potent anti‐inflammatory effects by inhibiting the secretion of interleukin 6 (IL‐6), interleukin 8 (IL‐8), and monocyte chemoattractant protein‐1 (MCP‐1). It also mitigated LPS‐induced oxidative stress by reducing the levels of phosphorylated Foxo3a (Ser253) and restored sirtuin 1 (SIRT1) expression. Notably, SIRT1 silencing abolished AnxA1's protective effects on Foxo3a phosphorylation and cellular senescence, suggesting that SIRT1 mediates AnxA1's actions. In conclusion, AnxA1 protected PDLCs against LPS‐triggered inflammation and cell senescence by activating SIRT1 signal pathway. These findings indicate that AnxA1 could serve as a promising therapeutic strategy for the treatment of periodontitis. [ABSTRACT FROM AUTHOR] |
| Copyright of Biotechnology & Applied Biochemistry is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
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| Header | DbId: egs DbLabel: Engineering Source An: 184297918 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Annexin A1 protects periodontal ligament cells against lipopolysaccharide‐induced inflammatory response and cellular senescence: An implication in periodontitis. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Luo%2C+Shuwen%22">Luo, Shuwen</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Zhang%2C+Lin%22">Zhang, Lin</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Li%2C+Xiaoyu%22">Li, Xiaoyu</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Tong%2C+Chunshi%22">Tong, Chunshi</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> tongchuns003@126.com</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Biotechnology+%26+Applied+Biochemistry%22">Biotechnology & Applied Biochemistry</searchLink>. Apr2025, Vol. 72 Issue 2, p449-459. 11p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Telomerase+reverse+transcriptase%22">Telomerase reverse transcriptase</searchLink><br /><searchLink fieldCode="DE" term="%22Cellular+aging%22">Cellular aging</searchLink><br /><searchLink fieldCode="DE" term="%22Sirtuins%22">Sirtuins</searchLink><br /><searchLink fieldCode="DE" term="%22Annexins%22">Annexins</searchLink><br /><searchLink fieldCode="DE" term="%22Periodontal+ligament%22">Periodontal ligament</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Periodontitis is an inflammatory condition that affects the tooth‐supporting structures, triggered by the host's immune response toward the bacterial deposits around the teeth. Annexin A1 (AnxA1), a vital member of the annexin superfamily, is known for its diverse physiological functions, particularly its anti‐inflammatory and anti‐senescence properties. We hypothesized that AnxA1 has a protective effect against lipopolysaccharide (LPS)‐induced inflammatory responses and cellular damage in periodontal ligament cells (PDLCs). In this study, we demonstrate that LPS stimulation significantly reduced telomerase activity in PDLCs, a decline that was dose‐dependently reversed by AnxA1. Importantly, AnxA1 protected the cells from LPS‐induced cellular senescence and the downregulation of human telomerase reverse transcriptase (hTERT) expression. In line with this, AnxA1 suppressed the LPS‐induced expression of p21 and p16 at both the mRNA and protein levels. Furthermore, AnxA1 demonstrated potent anti‐inflammatory effects by inhibiting the secretion of interleukin 6 (IL‐6), interleukin 8 (IL‐8), and monocyte chemoattractant protein‐1 (MCP‐1). It also mitigated LPS‐induced oxidative stress by reducing the levels of phosphorylated Foxo3a (Ser253) and restored sirtuin 1 (SIRT1) expression. Notably, SIRT1 silencing abolished AnxA1's protective effects on Foxo3a phosphorylation and cellular senescence, suggesting that SIRT1 mediates AnxA1's actions. In conclusion, AnxA1 protected PDLCs against LPS‐triggered inflammation and cell senescence by activating SIRT1 signal pathway. These findings indicate that AnxA1 could serve as a promising therapeutic strategy for the treatment of periodontitis. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Biotechnology & Applied Biochemistry is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1002/bab.2675 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 11 StartPage: 449 Subjects: – SubjectFull: Telomerase reverse transcriptase Type: general – SubjectFull: Cellular aging Type: general – SubjectFull: Sirtuins Type: general – SubjectFull: Annexins Type: general – SubjectFull: Periodontal ligament Type: general Titles: – TitleFull: Annexin A1 protects periodontal ligament cells against lipopolysaccharide‐induced inflammatory response and cellular senescence: An implication in periodontitis. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Luo, Shuwen – PersonEntity: Name: NameFull: Zhang, Lin – PersonEntity: Name: NameFull: Li, Xiaoyu – PersonEntity: Name: NameFull: Tong, Chunshi IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 04 Text: Apr2025 Type: published Y: 2025 Identifiers: – Type: issn-print Value: 08854513 Numbering: – Type: volume Value: 72 – Type: issue Value: 2 Titles: – TitleFull: Biotechnology & Applied Biochemistry Type: main |
| ResultId | 1 |
