View in EDS HTML Full Text PDF Full Text

Transient Lymphatic Remodeling Follows Sub-Ablative High-Frequency Irreversible Electroporation Therapy in a 4T1 Murine Model.

Saved in:
Bibliographic Details
Title: Transient Lymphatic Remodeling Follows Sub-Ablative High-Frequency Irreversible Electroporation Therapy in a 4T1 Murine Model.
Authors: Esparza, Savieay1,2 (AUTHOR), Jacobs, Edward2,3 (AUTHOR), Hammel, Jennifer H.1,2 (AUTHOR), Michelhaugh, Sharon K.1 (AUTHOR), Alinezhadbalalami, Nastaran2 (AUTHOR), Nagai-Singer, Margaret4 (AUTHOR), Imran, Khan Mohammad4 (AUTHOR), Davalos, Rafael V.2,3 (AUTHOR), Allen, Irving C.4 (AUTHOR), Verbridge, Scott S.2 (AUTHOR), Munson, Jennifer M.1,2 (AUTHOR) Jm4kt@vt.edu
Source: Annals of Biomedical Engineering. May2025, Vol. 53 Issue 5, p1148-1164. 17p.
Subjects: Electroporation therapy, Vascular remodeling, Lymph nodes, Ablation techniques, Macrophage inflammatory proteins, Electroporation
Abstract: High-frequency irreversible electroporation (H-FIRE) is a minimally invasive local ablation therapy known to activate the adaptive immune system and reprogram the tumor microenvironment. Its predecessor, irreversible electroporation (IRE), transiently increases microvascular density and immune cell infiltration within the surviving non-ablated and non-necrotic tumor region, also known as the viable tumor region. However, the impact of pulse electric field therapies on lymphatic vessels, crucial for T-cell fate and maturation, remains unclear. This study investigates how sub-ablative H-FIRE (SA-HFIRE) affects lymphatic and blood microvascular remodeling in the 4T1 mammary mouse model. We conducted a temporal and spatial analysis to evaluate vascular changes in the viable tumor, peritumoral fat pad, and tumor-draining lymph node post-treatment. Histological examination showed a transient increase in blood vessel density on Day 1 post-treatment, followed by a spike in lymphatic vessel density in the viable tumor region on Day 3 post-treatment, increased lymphatic vessel density in the peripheral fat pad, and minimal remodeling of the tumor-draining lymph node within 3 days following treatment. Gene expression analysis indicated elevated levels of CCL21 and CXCL2 on Day 1 post-treatment, while VEGFA and VEGFC did not appear to contribute to vascular remodeling. Likewise, CCL21 protein content in tumor-draining axillary lymph nodes correlated with gene expression data from the viable tumor region. These findings suggest a dynamic shift in lymphatic and blood microvascular structures post-SA-HFIRE, potentially enhancing the adaptive immune response through CCL21-mediated lymphatic homing and subsequent lymph node microvascular remodeling. Future work will assess the immune and transport function of the microvasculature to inform experiments aimed at the application of adjuvant therapies during scenarios of tumor partial ablation. [ABSTRACT FROM AUTHOR]
Copyright of Annals of Biomedical Engineering is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
Full text is not displayed to guests.
FullText Links:
  – Type: pdflink
Text:
  Availability: 1
Header DbId: egs
DbLabel: Engineering Source
An: 184556076
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 0
IllustrationInfo
Items – Name: Title
  Label: Title
  Group: Ti
  Data: Transient Lymphatic Remodeling Follows Sub-Ablative High-Frequency Irreversible Electroporation Therapy in a 4T1 Murine Model.
– Name: Author
  Label: Authors
  Group: Au
  Data: <searchLink fieldCode="AR" term="%22Esparza%2C+Savieay%22">Esparza, Savieay</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Jacobs%2C+Edward%22">Jacobs, Edward</searchLink><relatesTo>2,3</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Hammel%2C+Jennifer+H%2E%22">Hammel, Jennifer H.</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Michelhaugh%2C+Sharon+K%2E%22">Michelhaugh, Sharon K.</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Alinezhadbalalami%2C+Nastaran%22">Alinezhadbalalami, Nastaran</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Nagai-Singer%2C+Margaret%22">Nagai-Singer, Margaret</searchLink><relatesTo>4</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Imran%2C+Khan+Mohammad%22">Imran, Khan Mohammad</searchLink><relatesTo>4</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Davalos%2C+Rafael+V%2E%22">Davalos, Rafael V.</searchLink><relatesTo>2,3</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Allen%2C+Irving+C%2E%22">Allen, Irving C.</searchLink><relatesTo>4</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Verbridge%2C+Scott+S%2E%22">Verbridge, Scott S.</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Munson%2C+Jennifer+M%2E%22">Munson, Jennifer M.</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<i> Jm4kt@vt.edu</i>
– Name: TitleSource
  Label: Source
  Group: Src
  Data: <searchLink fieldCode="JN" term="%22Annals+of+Biomedical+Engineering%22">Annals of Biomedical Engineering</searchLink>. May2025, Vol. 53 Issue 5, p1148-1164. 17p.
– Name: Subject
  Label: Subjects
  Group: Su
  Data: <searchLink fieldCode="DE" term="%22Electroporation+therapy%22">Electroporation therapy</searchLink><br /><searchLink fieldCode="DE" term="%22Vascular+remodeling%22">Vascular remodeling</searchLink><br /><searchLink fieldCode="DE" term="%22Lymph+nodes%22">Lymph nodes</searchLink><br /><searchLink fieldCode="DE" term="%22Ablation+techniques%22">Ablation techniques</searchLink><br /><searchLink fieldCode="DE" term="%22Macrophage+inflammatory+proteins%22">Macrophage inflammatory proteins</searchLink><br /><searchLink fieldCode="DE" term="%22Electroporation%22">Electroporation</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: High-frequency irreversible electroporation (H-FIRE) is a minimally invasive local ablation therapy known to activate the adaptive immune system and reprogram the tumor microenvironment. Its predecessor, irreversible electroporation (IRE), transiently increases microvascular density and immune cell infiltration within the surviving non-ablated and non-necrotic tumor region, also known as the viable tumor region. However, the impact of pulse electric field therapies on lymphatic vessels, crucial for T-cell fate and maturation, remains unclear. This study investigates how sub-ablative H-FIRE (SA-HFIRE) affects lymphatic and blood microvascular remodeling in the 4T1 mammary mouse model. We conducted a temporal and spatial analysis to evaluate vascular changes in the viable tumor, peritumoral fat pad, and tumor-draining lymph node post-treatment. Histological examination showed a transient increase in blood vessel density on Day 1 post-treatment, followed by a spike in lymphatic vessel density in the viable tumor region on Day 3 post-treatment, increased lymphatic vessel density in the peripheral fat pad, and minimal remodeling of the tumor-draining lymph node within 3 days following treatment. Gene expression analysis indicated elevated levels of CCL21 and CXCL2 on Day 1 post-treatment, while VEGFA and VEGFC did not appear to contribute to vascular remodeling. Likewise, CCL21 protein content in tumor-draining axillary lymph nodes correlated with gene expression data from the viable tumor region. These findings suggest a dynamic shift in lymphatic and blood microvascular structures post-SA-HFIRE, potentially enhancing the adaptive immune response through CCL21-mediated lymphatic homing and subsequent lymph node microvascular remodeling. Future work will assess the immune and transport function of the microvasculature to inform experiments aimed at the application of adjuvant therapies during scenarios of tumor partial ablation. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Annals of Biomedical Engineering is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
PLink https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=egs&AN=184556076
RecordInfo BibRecord:
  BibEntity:
    Identifiers:
      – Type: doi
        Value: 10.1007/s10439-024-03674-y
    Languages:
      – Code: eng
        Text: English
    PhysicalDescription:
      Pagination:
        PageCount: 17
        StartPage: 1148
    Subjects:
      – SubjectFull: Electroporation therapy
        Type: general
      – SubjectFull: Vascular remodeling
        Type: general
      – SubjectFull: Lymph nodes
        Type: general
      – SubjectFull: Ablation techniques
        Type: general
      – SubjectFull: Macrophage inflammatory proteins
        Type: general
      – SubjectFull: Electroporation
        Type: general
    Titles:
      – TitleFull: Transient Lymphatic Remodeling Follows Sub-Ablative High-Frequency Irreversible Electroporation Therapy in a 4T1 Murine Model.
        Type: main
  BibRelationships:
    HasContributorRelationships:
      – PersonEntity:
          Name:
            NameFull: Esparza, Savieay
      – PersonEntity:
          Name:
            NameFull: Jacobs, Edward
      – PersonEntity:
          Name:
            NameFull: Hammel, Jennifer H.
      – PersonEntity:
          Name:
            NameFull: Michelhaugh, Sharon K.
      – PersonEntity:
          Name:
            NameFull: Alinezhadbalalami, Nastaran
      – PersonEntity:
          Name:
            NameFull: Nagai-Singer, Margaret
      – PersonEntity:
          Name:
            NameFull: Imran, Khan Mohammad
      – PersonEntity:
          Name:
            NameFull: Davalos, Rafael V.
      – PersonEntity:
          Name:
            NameFull: Allen, Irving C.
      – PersonEntity:
          Name:
            NameFull: Verbridge, Scott S.
      – PersonEntity:
          Name:
            NameFull: Munson, Jennifer M.
    IsPartOfRelationships:
      – BibEntity:
          Dates:
            – D: 01
              M: 05
              Text: May2025
              Type: published
              Y: 2025
          Identifiers:
            – Type: issn-print
              Value: 00906964
          Numbering:
            – Type: volume
              Value: 53
            – Type: issue
              Value: 5
          Titles:
            – TitleFull: Annals of Biomedical Engineering
              Type: main
ResultId 1