Parasitoid Calyx Fluid and Venom Affect Bacterial Communities in Their Lepidopteran Host Labial Salivary Glands.
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| Title: | Parasitoid Calyx Fluid and Venom Affect Bacterial Communities in Their Lepidopteran Host Labial Salivary Glands. |
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| Authors: | Cuny, Maximilien A. C.1,2 (AUTHOR) maximilien.adam@cirad.fr, Gloder, Gabriele3,4 (AUTHOR) gabriele.gloder@kuleuven.be, Bourne, Mitchel E.1,5 (AUTHOR) mitchel.bourne@uzh.ch, Kalisvaart, Sarah N.1 (AUTHOR) sarah.kalisvaart@wur.nl, Verreth, Christel3,4 (AUTHOR) christel.verreth@kuleuven.be, Crauwels, Sam3,4 (AUTHOR) sam.crauwels@kuleuven.be, Cusumano, Antonino6 (AUTHOR) antonino.cusumano@unipa.it, Lievens, Bart3,4 (AUTHOR) bart.lievens@kuleuven.be, Poelman, Erik H.1 (AUTHOR) erik.poelman@wur.nl |
| Source: | Microbial Ecology. 4/23/2025, Vol. 88 Issue 1, p1-12. 12p. |
| Subjects: | Bacterial communities, Insect physiology, Insect communities, Fluid injection, Venom, Salivary glands |
| Abstract: | The influence of gut and gonad bacterial communities on insect physiology, behaviour, and ecology is increasingly recognised. Parasitism by parasitoid wasps alters many physiological processes in their hosts, including gut bacterial communities. However, it remains unclear whether these changes are restricted to the gut or also occur in other tissues and fluids, and the mechanisms underlying such changes are unknown. We hypothesise that host microbiome changes result from the injection of calyx fluid (that contain symbiotic viruses known as polydnaviruses) and venom during parasitoid oviposition and that these effects vary by host tissue. To test this, we microinjected Pieris brassicae caterpillars with calyx fluid and venom from Cotesia glomerata, using saline solution and natural parasitism by C. glomerata as controls. We analysed changes in the bacterial community composition in the gut, regurgitate, haemolymph, and labial salivary glands of the host insects. Multivariate analysis revealed distinct bacterial communities across tissues and fluids, with high diversity in the salivary glands and haemolymph. Parasitism and injection of calyx fluid and venom significantly altered bacterial communities in the salivary glands. Differential abundance analysis showed that parasitism affected bacterial relative abundance in the haemolymph, and that Wolbachia was only found in the haemolymph of parasitized caterpillars. Altogether, our findings reveal that parasitism influences the host haemolymph microbiome, and both parasitism and injection of calyx fluid and venom drive changes in the bacterial community composition within the host salivary glands. Given that the composition of salivary glands can influence plant response to herbivory, we discuss these results in the broader context of plant-parasitoid interactions. [ABSTRACT FROM AUTHOR] |
| Copyright of Microbial Ecology is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
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| Header | DbId: egs DbLabel: Engineering Source An: 184707188 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Parasitoid Calyx Fluid and Venom Affect Bacterial Communities in Their Lepidopteran Host Labial Salivary Glands. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Cuny%2C+Maximilien+A%2E+C%2E%22">Cuny, Maximilien A. C.</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<i> maximilien.adam@cirad.fr</i><br /><searchLink fieldCode="AR" term="%22Gloder%2C+Gabriele%22">Gloder, Gabriele</searchLink><relatesTo>3,4</relatesTo> (AUTHOR)<i> gabriele.gloder@kuleuven.be</i><br /><searchLink fieldCode="AR" term="%22Bourne%2C+Mitchel+E%2E%22">Bourne, Mitchel E.</searchLink><relatesTo>1,5</relatesTo> (AUTHOR)<i> mitchel.bourne@uzh.ch</i><br /><searchLink fieldCode="AR" term="%22Kalisvaart%2C+Sarah+N%2E%22">Kalisvaart, Sarah N.</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> sarah.kalisvaart@wur.nl</i><br /><searchLink fieldCode="AR" term="%22Verreth%2C+Christel%22">Verreth, Christel</searchLink><relatesTo>3,4</relatesTo> (AUTHOR)<i> christel.verreth@kuleuven.be</i><br /><searchLink fieldCode="AR" term="%22Crauwels%2C+Sam%22">Crauwels, Sam</searchLink><relatesTo>3,4</relatesTo> (AUTHOR)<i> sam.crauwels@kuleuven.be</i><br /><searchLink fieldCode="AR" term="%22Cusumano%2C+Antonino%22">Cusumano, Antonino</searchLink><relatesTo>6</relatesTo> (AUTHOR)<i> antonino.cusumano@unipa.it</i><br /><searchLink fieldCode="AR" term="%22Lievens%2C+Bart%22">Lievens, Bart</searchLink><relatesTo>3,4</relatesTo> (AUTHOR)<i> bart.lievens@kuleuven.be</i><br /><searchLink fieldCode="AR" term="%22Poelman%2C+Erik+H%2E%22">Poelman, Erik H.</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> erik.poelman@wur.nl</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Microbial+Ecology%22">Microbial Ecology</searchLink>. 4/23/2025, Vol. 88 Issue 1, p1-12. 12p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Bacterial+communities%22">Bacterial communities</searchLink><br /><searchLink fieldCode="DE" term="%22Insect+physiology%22">Insect physiology</searchLink><br /><searchLink fieldCode="DE" term="%22Insect+communities%22">Insect communities</searchLink><br /><searchLink fieldCode="DE" term="%22Fluid+injection%22">Fluid injection</searchLink><br /><searchLink fieldCode="DE" term="%22Venom%22">Venom</searchLink><br /><searchLink fieldCode="DE" term="%22Salivary+glands%22">Salivary glands</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: The influence of gut and gonad bacterial communities on insect physiology, behaviour, and ecology is increasingly recognised. Parasitism by parasitoid wasps alters many physiological processes in their hosts, including gut bacterial communities. However, it remains unclear whether these changes are restricted to the gut or also occur in other tissues and fluids, and the mechanisms underlying such changes are unknown. We hypothesise that host microbiome changes result from the injection of calyx fluid (that contain symbiotic viruses known as polydnaviruses) and venom during parasitoid oviposition and that these effects vary by host tissue. To test this, we microinjected Pieris brassicae caterpillars with calyx fluid and venom from Cotesia glomerata, using saline solution and natural parasitism by C. glomerata as controls. We analysed changes in the bacterial community composition in the gut, regurgitate, haemolymph, and labial salivary glands of the host insects. Multivariate analysis revealed distinct bacterial communities across tissues and fluids, with high diversity in the salivary glands and haemolymph. Parasitism and injection of calyx fluid and venom significantly altered bacterial communities in the salivary glands. Differential abundance analysis showed that parasitism affected bacterial relative abundance in the haemolymph, and that Wolbachia was only found in the haemolymph of parasitized caterpillars. Altogether, our findings reveal that parasitism influences the host haemolymph microbiome, and both parasitism and injection of calyx fluid and venom drive changes in the bacterial community composition within the host salivary glands. Given that the composition of salivary glands can influence plant response to herbivory, we discuss these results in the broader context of plant-parasitoid interactions. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Microbial Ecology is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1007/s00248-025-02535-y Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 12 StartPage: 1 Subjects: – SubjectFull: Bacterial communities Type: general – SubjectFull: Insect physiology Type: general – SubjectFull: Insect communities Type: general – SubjectFull: Fluid injection Type: general – SubjectFull: Venom Type: general – SubjectFull: Salivary glands Type: general Titles: – TitleFull: Parasitoid Calyx Fluid and Venom Affect Bacterial Communities in Their Lepidopteran Host Labial Salivary Glands. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Cuny, Maximilien A. C. – PersonEntity: Name: NameFull: Gloder, Gabriele – PersonEntity: Name: NameFull: Bourne, Mitchel E. – PersonEntity: Name: NameFull: Kalisvaart, Sarah N. – PersonEntity: Name: NameFull: Verreth, Christel – PersonEntity: Name: NameFull: Crauwels, Sam – PersonEntity: Name: NameFull: Cusumano, Antonino – PersonEntity: Name: NameFull: Lievens, Bart – PersonEntity: Name: NameFull: Poelman, Erik H. IsPartOfRelationships: – BibEntity: Dates: – D: 23 M: 04 Text: 4/23/2025 Type: published Y: 2025 Identifiers: – Type: issn-print Value: 00953628 Numbering: – Type: volume Value: 88 – Type: issue Value: 1 Titles: – TitleFull: Microbial Ecology Type: main |
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