3D microcapsules for human bone marrow-derived mesenchymal stem cell biomanufacturing in a vertical-wheel bioreactor.
Saved in:
| Title: | 3D microcapsules for human bone marrow-derived mesenchymal stem cell biomanufacturing in a vertical-wheel bioreactor. |
|---|---|
| Authors: | Teryek, Matthew1 (AUTHOR), Jadhav, Pankaj2 (AUTHOR), Bento, Raphaela1 (AUTHOR), Parekkadan, Biju1 (AUTHOR) biju.parekkadan@rutgers.edu |
| Source: | Biotechnology & Bioprocess Engineering. Apr2025, Vol. 30 Issue 2, p319-335. 17p. |
| Subjects: | Mesenchymal stem cells, Stromal cells, Green business, Regenerative medicine, Microencapsulation |
| Abstract: | Microencapsulation of human mesenchymal stromal cells (MSCs) via electrospraying has been well documented in tissue engineering and regenerative medicine. Herein, we report the use of microencapsulation via electrospraying, for MSC expansion using a commercially available hydrogel that is durable, optimized to MSC culture, and enzymatically degradable for cell recovery. Critical parameters of the electrospraying encapsulation process such as seeding density, correlation of microcapsule output with hydrogel volume, and applied voltage were characterized to consistently fabricate cell-laden microcapsules of uniform size. Upon encapsulation, we quantified ~ 10× expansion of encapsulated MSCs within a vertical-wheel bioreactor, and verified the preservation of critical quality attributes including immunophenotype and multipotency after expansion and cell recovery. Finally, we highlight the genetic manipulation of encapsulated MSCs as an example of incorporating bioactive agents in the microcapsule to create new compositions of MSCs with altered phenotypes. [ABSTRACT FROM AUTHOR] |
| Copyright of Biotechnology & Bioprocess Engineering is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Links: – Type: pdflink Text: Availability: 0 |
|---|---|
| Header | DbId: egs DbLabel: Engineering Source An: 184745357 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
| IllustrationInfo | |
| Items | – Name: Title Label: Title Group: Ti Data: 3D microcapsules for human bone marrow-derived mesenchymal stem cell biomanufacturing in a vertical-wheel bioreactor. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Teryek%2C+Matthew%22">Teryek, Matthew</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Jadhav%2C+Pankaj%22">Jadhav, Pankaj</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Bento%2C+Raphaela%22">Bento, Raphaela</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Parekkadan%2C+Biju%22">Parekkadan, Biju</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> biju.parekkadan@rutgers.edu</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Biotechnology+%26+Bioprocess+Engineering%22">Biotechnology & Bioprocess Engineering</searchLink>. Apr2025, Vol. 30 Issue 2, p319-335. 17p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Mesenchymal+stem+cells%22">Mesenchymal stem cells</searchLink><br /><searchLink fieldCode="DE" term="%22Stromal+cells%22">Stromal cells</searchLink><br /><searchLink fieldCode="DE" term="%22Green+business%22">Green business</searchLink><br /><searchLink fieldCode="DE" term="%22Regenerative+medicine%22">Regenerative medicine</searchLink><br /><searchLink fieldCode="DE" term="%22Microencapsulation%22">Microencapsulation</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Microencapsulation of human mesenchymal stromal cells (MSCs) via electrospraying has been well documented in tissue engineering and regenerative medicine. Herein, we report the use of microencapsulation via electrospraying, for MSC expansion using a commercially available hydrogel that is durable, optimized to MSC culture, and enzymatically degradable for cell recovery. Critical parameters of the electrospraying encapsulation process such as seeding density, correlation of microcapsule output with hydrogel volume, and applied voltage were characterized to consistently fabricate cell-laden microcapsules of uniform size. Upon encapsulation, we quantified ~ 10× expansion of encapsulated MSCs within a vertical-wheel bioreactor, and verified the preservation of critical quality attributes including immunophenotype and multipotency after expansion and cell recovery. Finally, we highlight the genetic manipulation of encapsulated MSCs as an example of incorporating bioactive agents in the microcapsule to create new compositions of MSCs with altered phenotypes. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Biotechnology & Bioprocess Engineering is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
| PLink | https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=egs&AN=184745357 |
| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1007/s12257-024-00069-7 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 17 StartPage: 319 Subjects: – SubjectFull: Mesenchymal stem cells Type: general – SubjectFull: Stromal cells Type: general – SubjectFull: Green business Type: general – SubjectFull: Regenerative medicine Type: general – SubjectFull: Microencapsulation Type: general Titles: – TitleFull: 3D microcapsules for human bone marrow-derived mesenchymal stem cell biomanufacturing in a vertical-wheel bioreactor. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Teryek, Matthew – PersonEntity: Name: NameFull: Jadhav, Pankaj – PersonEntity: Name: NameFull: Bento, Raphaela – PersonEntity: Name: NameFull: Parekkadan, Biju IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 04 Text: Apr2025 Type: published Y: 2025 Identifiers: – Type: issn-print Value: 12268372 Numbering: – Type: volume Value: 30 – Type: issue Value: 2 Titles: – TitleFull: Biotechnology & Bioprocess Engineering Type: main |
| ResultId | 1 |
