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Nonlinear Magnetic Response Measurements in Study of Magnetic Nanoparticles Uptake by Mesenchymal Stem Cells.

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Title: Nonlinear Magnetic Response Measurements in Study of Magnetic Nanoparticles Uptake by Mesenchymal Stem Cells.
Authors: Ryzhov, Vyacheslav1 (AUTHOR) marchenko_yy@pnpi.nrcki.ru, Marchenko, Yaroslav1,2 (AUTHOR), Deriglazov, Vladimir1,3 (AUTHOR), Yudintceva, Natalia2,4 (AUTHOR), Smirnov, Oleg1,5 (AUTHOR), Arutyunyan, Alexandr1 (AUTHOR), Shtam, Tatiana1,2 (AUTHOR), Ivanov, Evgenii3 (AUTHOR), Combs, Stephanie E.4 (AUTHOR), Shevtsov, Maxim2,4,5 (AUTHOR) maxim.shevtsov@tum.de
Source: Nanomaterials (2079-4991). May2025, Vol. 15 Issue 9, p675. 16p.
Subjects: Mesenchymal stem cells, Magnetic measurements, Iron oxide nanoparticles, Magnetic fields, Magnetic nanoparticles
Abstract: Stem cells therapies offer a promising approach in translational oncology, as well as in regenerative medicine due to the tropism of these cells to the damage site. To track the distribution of stem cells, the latter could be labeled by MRI-sensitive superparamagnetic (SPM) iron oxide nanoparticles. In the current study, magnetic properties of the magnetic nanoparticles (MNPs) incorporated into the bone marrow-derived fetal mesenchymal stem cells (FetMSCs) were evaluated employing nonlinear magnetic response measurements. Synthesized dextran-coated iron oxide nanoparticles were additionally characterized by X-ray diffraction, transmission electron microscopy, and dynamic light scattering. The MNP uptake by the FetMSCs 24 h following coincubation was studied by longitudinal nonlinear response to weak alternating magnetic field with registration of the second harmonic of magnetization. Subsequent data processing using a formalism based on the numerical solution of the Fokker–Planck kinetic equation allowed us to determine magnetic and dynamic parameters and the state of MNPs in the cells, as well as in the culture medium. It was found that MNPs formed aggregates in the culture medium; they were absorbed by the cells during coincubation. The aggregates exhibited SPM regime in the medium, and the parameters of the MNP aggregates remained virtually unchanged in the cells, indicating the preservation of the aggregation state of MNPs inside the cells. This implies also the preservation of the organic shell of the nanoparticles inside FetMSCs. The accumulation of MNPs by mesenchymal stem cells gradually increased with the concentration of MNPs. Thus, the study confirmed that the labeling of MSCs with MNPs is an effective method for subsequent cell tracking as incorporated nanoparticles retain their magnetic properties. [ABSTRACT FROM AUTHOR]
Copyright of Nanomaterials (2079-4991) is the property of MDPI and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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  Data: Nonlinear Magnetic Response Measurements in Study of Magnetic Nanoparticles Uptake by Mesenchymal Stem Cells.
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  Data: <searchLink fieldCode="AR" term="%22Ryzhov%2C+Vyacheslav%22">Ryzhov, Vyacheslav</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> marchenko_yy@pnpi.nrcki.ru</i><br /><searchLink fieldCode="AR" term="%22Marchenko%2C+Yaroslav%22">Marchenko, Yaroslav</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Deriglazov%2C+Vladimir%22">Deriglazov, Vladimir</searchLink><relatesTo>1,3</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Yudintceva%2C+Natalia%22">Yudintceva, Natalia</searchLink><relatesTo>2,4</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Smirnov%2C+Oleg%22">Smirnov, Oleg</searchLink><relatesTo>1,5</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Arutyunyan%2C+Alexandr%22">Arutyunyan, Alexandr</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Shtam%2C+Tatiana%22">Shtam, Tatiana</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Ivanov%2C+Evgenii%22">Ivanov, Evgenii</searchLink><relatesTo>3</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Combs%2C+Stephanie+E%2E%22">Combs, Stephanie E.</searchLink><relatesTo>4</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Shevtsov%2C+Maxim%22">Shevtsov, Maxim</searchLink><relatesTo>2,4,5</relatesTo> (AUTHOR)<i> maxim.shevtsov@tum.de</i>
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  Data: <searchLink fieldCode="JN" term="%22Nanomaterials+%282079-4991%29%22">Nanomaterials (2079-4991)</searchLink>. May2025, Vol. 15 Issue 9, p675. 16p.
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  Data: <searchLink fieldCode="DE" term="%22Mesenchymal+stem+cells%22">Mesenchymal stem cells</searchLink><br /><searchLink fieldCode="DE" term="%22Magnetic+measurements%22">Magnetic measurements</searchLink><br /><searchLink fieldCode="DE" term="%22Iron+oxide+nanoparticles%22">Iron oxide nanoparticles</searchLink><br /><searchLink fieldCode="DE" term="%22Magnetic+fields%22">Magnetic fields</searchLink><br /><searchLink fieldCode="DE" term="%22Magnetic+nanoparticles%22">Magnetic nanoparticles</searchLink>
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  Data: Stem cells therapies offer a promising approach in translational oncology, as well as in regenerative medicine due to the tropism of these cells to the damage site. To track the distribution of stem cells, the latter could be labeled by MRI-sensitive superparamagnetic (SPM) iron oxide nanoparticles. In the current study, magnetic properties of the magnetic nanoparticles (MNPs) incorporated into the bone marrow-derived fetal mesenchymal stem cells (FetMSCs) were evaluated employing nonlinear magnetic response measurements. Synthesized dextran-coated iron oxide nanoparticles were additionally characterized by X-ray diffraction, transmission electron microscopy, and dynamic light scattering. The MNP uptake by the FetMSCs 24 h following coincubation was studied by longitudinal nonlinear response to weak alternating magnetic field with registration of the second harmonic of magnetization. Subsequent data processing using a formalism based on the numerical solution of the Fokker–Planck kinetic equation allowed us to determine magnetic and dynamic parameters and the state of MNPs in the cells, as well as in the culture medium. It was found that MNPs formed aggregates in the culture medium; they were absorbed by the cells during coincubation. The aggregates exhibited SPM regime in the medium, and the parameters of the MNP aggregates remained virtually unchanged in the cells, indicating the preservation of the aggregation state of MNPs inside the cells. This implies also the preservation of the organic shell of the nanoparticles inside FetMSCs. The accumulation of MNPs by mesenchymal stem cells gradually increased with the concentration of MNPs. Thus, the study confirmed that the labeling of MSCs with MNPs is an effective method for subsequent cell tracking as incorporated nanoparticles retain their magnetic properties. [ABSTRACT FROM AUTHOR]
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  Data: <i>Copyright of Nanomaterials (2079-4991) is the property of MDPI and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Value: 10.3390/nano15090675
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        Text: English
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        PageCount: 16
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    Subjects:
      – SubjectFull: Mesenchymal stem cells
        Type: general
      – SubjectFull: Magnetic measurements
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      – SubjectFull: Iron oxide nanoparticles
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      – SubjectFull: Magnetic fields
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              Text: May2025
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