Critical Design Parameters of Tantalum-Based Comb Structures to Manipulate Mammalian Cell Morphology.
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| Title: | Critical Design Parameters of Tantalum-Based Comb Structures to Manipulate Mammalian Cell Morphology. |
|---|---|
| Authors: | Moussa, Hassan I.1,2 (AUTHOR), Logan, Megan1,2 (AUTHOR), Eskandari, Ali1,2,3 (AUTHOR), Glerum, D. Moira1,2,3 (AUTHOR) marc.aucoin@uwaterloo.ca, Aucoin, Marc G.1,2 (AUTHOR), Tsui, Ting Y.1,2,3 (AUTHOR) tttsui@uwaterloo.ca |
| Source: | Materials (1996-1944). May2025, Vol. 18 Issue 9, p2099. 18p. |
| Subjects: | Cercopithecus aethiops, Cell morphology, Orthopedic implants, Tantalum alloys, Scanning electron microscopy |
| Abstract: | Mammalian tissues and cells often orient naturally in specific patterns to function effectively. This cellular alignment is influenced by the chemical nature and topographic features of the extracellular matrix. In implants, a range of different materials have been used in vivo. Of those, tantalum and its alloys are promising materials, especially in orthopedic implant applications. Previous studies have demonstrated that nano- and micro-scale surface features, such as symmetric comb structures, can significantly affect cell behavior and alignment. However, patterning need not be restricted to symmetric geometries, and there remains a gap in knowledge regarding how cells respond to asymmetric comb structures, where the widths of the trenches and lines in the comb differ. This study aims to address this gap by examining how Vero cells (cells derived from an African green monkey) respond when applied to tantalum and tantalum/silicon oxide asymmetric comb structures having fixed trench widths of 1 μm and line widths ranging from 3 μm to 50 μm. We also look at the cell responses on inverted patterns, where the line widths were fixed at 1 μm while trench widths varied. The orientation and morphology of the adherent cells were analyzed using fluorescence confocal microscopy and scanning electron microscopy. Our results indicate that the widths of the trenches and lines are important design parameters influencing cell behavior on asymmetric comb structures. Furthermore, the ability to manipulate cell morphology using these structures decreased when parts of the tantalum lines were replaced with silicon oxide. [ABSTRACT FROM AUTHOR] |
| Copyright of Materials (1996-1944) is the property of MDPI and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
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| Header | DbId: egs DbLabel: Engineering Source An: 185131654 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Critical Design Parameters of Tantalum-Based Comb Structures to Manipulate Mammalian Cell Morphology. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Moussa%2C+Hassan+I%2E%22">Moussa, Hassan I.</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Logan%2C+Megan%22">Logan, Megan</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Eskandari%2C+Ali%22">Eskandari, Ali</searchLink><relatesTo>1,2,3</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Glerum%2C+D%2E+Moira%22">Glerum, D. Moira</searchLink><relatesTo>1,2,3</relatesTo> (AUTHOR)<i> marc.aucoin@uwaterloo.ca</i><br /><searchLink fieldCode="AR" term="%22Aucoin%2C+Marc+G%2E%22">Aucoin, Marc G.</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Tsui%2C+Ting+Y%2E%22">Tsui, Ting Y.</searchLink><relatesTo>1,2,3</relatesTo> (AUTHOR)<i> tttsui@uwaterloo.ca</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Materials+%281996-1944%29%22">Materials (1996-1944)</searchLink>. May2025, Vol. 18 Issue 9, p2099. 18p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Cercopithecus+aethiops%22">Cercopithecus aethiops</searchLink><br /><searchLink fieldCode="DE" term="%22Cell+morphology%22">Cell morphology</searchLink><br /><searchLink fieldCode="DE" term="%22Orthopedic+implants%22">Orthopedic implants</searchLink><br /><searchLink fieldCode="DE" term="%22Tantalum+alloys%22">Tantalum alloys</searchLink><br /><searchLink fieldCode="DE" term="%22Scanning+electron+microscopy%22">Scanning electron microscopy</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Mammalian tissues and cells often orient naturally in specific patterns to function effectively. This cellular alignment is influenced by the chemical nature and topographic features of the extracellular matrix. In implants, a range of different materials have been used in vivo. Of those, tantalum and its alloys are promising materials, especially in orthopedic implant applications. Previous studies have demonstrated that nano- and micro-scale surface features, such as symmetric comb structures, can significantly affect cell behavior and alignment. However, patterning need not be restricted to symmetric geometries, and there remains a gap in knowledge regarding how cells respond to asymmetric comb structures, where the widths of the trenches and lines in the comb differ. This study aims to address this gap by examining how Vero cells (cells derived from an African green monkey) respond when applied to tantalum and tantalum/silicon oxide asymmetric comb structures having fixed trench widths of 1 μm and line widths ranging from 3 μm to 50 μm. We also look at the cell responses on inverted patterns, where the line widths were fixed at 1 μm while trench widths varied. The orientation and morphology of the adherent cells were analyzed using fluorescence confocal microscopy and scanning electron microscopy. Our results indicate that the widths of the trenches and lines are important design parameters influencing cell behavior on asymmetric comb structures. Furthermore, the ability to manipulate cell morphology using these structures decreased when parts of the tantalum lines were replaced with silicon oxide. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Materials (1996-1944) is the property of MDPI and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.3390/ma18092099 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 18 StartPage: 2099 Subjects: – SubjectFull: Cercopithecus aethiops Type: general – SubjectFull: Cell morphology Type: general – SubjectFull: Orthopedic implants Type: general – SubjectFull: Tantalum alloys Type: general – SubjectFull: Scanning electron microscopy Type: general Titles: – TitleFull: Critical Design Parameters of Tantalum-Based Comb Structures to Manipulate Mammalian Cell Morphology. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Moussa, Hassan I. – PersonEntity: Name: NameFull: Logan, Megan – PersonEntity: Name: NameFull: Eskandari, Ali – PersonEntity: Name: NameFull: Glerum, D. Moira – PersonEntity: Name: NameFull: Aucoin, Marc G. – PersonEntity: Name: NameFull: Tsui, Ting Y. IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 05 Text: May2025 Type: published Y: 2025 Identifiers: – Type: issn-print Value: 19961944 Numbering: – Type: volume Value: 18 – Type: issue Value: 9 Titles: – TitleFull: Materials (1996-1944) Type: main |
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