Molecular insights into the effects of laser-induced optical breakdown (LIOB) after 1064 nm picosecond laser irradiation using a novel melanocyte-containing 3D skin model.
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| Title: | Molecular insights into the effects of laser-induced optical breakdown (LIOB) after 1064 nm picosecond laser irradiation using a novel melanocyte-containing 3D skin model. |
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| Authors: | Huth, Sebastian1 (AUTHOR) shuth@ukaachen.de, Marquardt, Yvonne1 (AUTHOR) ymarquardt@ukaachen.de, Huth, Laura1 (AUTHOR) lhuth@ukaachen.de, Gerber, Peter Arne2 (AUTHOR) prof.gerber@dermatologie-am-luegplatz.de, Baron, Jens Malte1,3 (AUTHOR) jbaron@ukaachen.de |
| Source: | Lasers in Medical Science. 5/13/2025, Vol. 40 Issue 1, p1-9. 9p. |
| Subjects: | Diffractive optical elements, Heat shock proteins, Matrix metalloproteinases, YAG lasers, Skin regeneration |
| Abstract: | Picosecond lasers use a mechanism known as laser-induced optical breakdown (LIOB). However, the underlying molecular mechanisms are not yet fully understood. The aim of this study was to gain insights into the molecular effects of LIOB using novel melanocyte-containing 3D skin models. Since the threshold of LIOB depends on the melanin content of the skin, we established a new human 3D skin model comprising melanocytes. Irradiation was done with a diffractive optical elements (DOE-) assisted fractional 1064 nm Nd: YAG picosecond laser utilizing the energy setting of 0.2 J/cm2, with a spot size of 7 × 7 mm and one pulse per area. In a further approach, we post-treated the models topically with a dexpanthenol-containing ointment. Examination was done histologically and using next-generation sequencing. The histological analysis revealed intra-epidermal vacuoles with an intact environment immediately after irradiation of the models and even after 24 h. Post-treatment with the dexpanthenol-containing ointment accelerated the repair processes in the models, with vacuoles no longer visible after 24 h. We found an upregulation of matrix metalloproteinases, collagens, heat shock proteins, cytokines and chemokines, reflecting repair mechanisms and tissue remodeling after picosecond laser irradiation. Initial stimulation effects of laser therapy were maintained even after topical dexpanthenol post-treatment. We present the first in vitro study investigating the effects of LIOB after 1064 nm picosecond laser irradiation using a novel standardized melanocyte-containing 3D skin model. LIOB-induced intraepidermal vacuoles promoted skin regeneration processes, which could be supported and accelerated by post-treatment with a dexpanthenol-containing ointment. [ABSTRACT FROM AUTHOR] |
| Copyright of Lasers in Medical Science is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
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| Header | DbId: egs DbLabel: Engineering Source An: 185134529 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Molecular insights into the effects of laser-induced optical breakdown (LIOB) after 1064 nm picosecond laser irradiation using a novel melanocyte-containing 3D skin model. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Huth%2C+Sebastian%22">Huth, Sebastian</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> shuth@ukaachen.de</i><br /><searchLink fieldCode="AR" term="%22Marquardt%2C+Yvonne%22">Marquardt, Yvonne</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> ymarquardt@ukaachen.de</i><br /><searchLink fieldCode="AR" term="%22Huth%2C+Laura%22">Huth, Laura</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> lhuth@ukaachen.de</i><br /><searchLink fieldCode="AR" term="%22Gerber%2C+Peter+Arne%22">Gerber, Peter Arne</searchLink><relatesTo>2</relatesTo> (AUTHOR)<i> prof.gerber@dermatologie-am-luegplatz.de</i><br /><searchLink fieldCode="AR" term="%22Baron%2C+Jens+Malte%22">Baron, Jens Malte</searchLink><relatesTo>1,3</relatesTo> (AUTHOR)<i> jbaron@ukaachen.de</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Lasers+in+Medical+Science%22">Lasers in Medical Science</searchLink>. 5/13/2025, Vol. 40 Issue 1, p1-9. 9p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Diffractive+optical+elements%22">Diffractive optical elements</searchLink><br /><searchLink fieldCode="DE" term="%22Heat+shock+proteins%22">Heat shock proteins</searchLink><br /><searchLink fieldCode="DE" term="%22Matrix+metalloproteinases%22">Matrix metalloproteinases</searchLink><br /><searchLink fieldCode="DE" term="%22YAG+lasers%22">YAG lasers</searchLink><br /><searchLink fieldCode="DE" term="%22Skin+regeneration%22">Skin regeneration</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Picosecond lasers use a mechanism known as laser-induced optical breakdown (LIOB). However, the underlying molecular mechanisms are not yet fully understood. The aim of this study was to gain insights into the molecular effects of LIOB using novel melanocyte-containing 3D skin models. Since the threshold of LIOB depends on the melanin content of the skin, we established a new human 3D skin model comprising melanocytes. Irradiation was done with a diffractive optical elements (DOE-) assisted fractional 1064 nm Nd: YAG picosecond laser utilizing the energy setting of 0.2 J/cm2, with a spot size of 7 × 7 mm and one pulse per area. In a further approach, we post-treated the models topically with a dexpanthenol-containing ointment. Examination was done histologically and using next-generation sequencing. The histological analysis revealed intra-epidermal vacuoles with an intact environment immediately after irradiation of the models and even after 24 h. Post-treatment with the dexpanthenol-containing ointment accelerated the repair processes in the models, with vacuoles no longer visible after 24 h. We found an upregulation of matrix metalloproteinases, collagens, heat shock proteins, cytokines and chemokines, reflecting repair mechanisms and tissue remodeling after picosecond laser irradiation. Initial stimulation effects of laser therapy were maintained even after topical dexpanthenol post-treatment. We present the first in vitro study investigating the effects of LIOB after 1064 nm picosecond laser irradiation using a novel standardized melanocyte-containing 3D skin model. LIOB-induced intraepidermal vacuoles promoted skin regeneration processes, which could be supported and accelerated by post-treatment with a dexpanthenol-containing ointment. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Lasers in Medical Science is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1007/s10103-025-04474-z Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 9 StartPage: 1 Subjects: – SubjectFull: Diffractive optical elements Type: general – SubjectFull: Heat shock proteins Type: general – SubjectFull: Matrix metalloproteinases Type: general – SubjectFull: YAG lasers Type: general – SubjectFull: Skin regeneration Type: general Titles: – TitleFull: Molecular insights into the effects of laser-induced optical breakdown (LIOB) after 1064 nm picosecond laser irradiation using a novel melanocyte-containing 3D skin model. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Huth, Sebastian – PersonEntity: Name: NameFull: Marquardt, Yvonne – PersonEntity: Name: NameFull: Huth, Laura – PersonEntity: Name: NameFull: Gerber, Peter Arne – PersonEntity: Name: NameFull: Baron, Jens Malte IsPartOfRelationships: – BibEntity: Dates: – D: 13 M: 05 Text: 5/13/2025 Type: published Y: 2025 Identifiers: – Type: issn-print Value: 02688921 Numbering: – Type: volume Value: 40 – Type: issue Value: 1 Titles: – TitleFull: Lasers in Medical Science Type: main |
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