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Molecular characterization and validation of adult-plant stripe rust resistance genes in Yunnan hulled wheat (Triticum aestivum ssp. yunnanense King)

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Title: Molecular characterization and validation of adult-plant stripe rust resistance genes in Yunnan hulled wheat (Triticum aestivum ssp. yunnanense King)
Authors: Huang, Linyu1,2 (AUTHOR), Wang, Xinyu2 (AUTHOR), Yan, Yan2 (AUTHOR), Long, Li2 (AUTHOR), Li, Qiushuo2 (AUTHOR), Wang, Yuexue2 (AUTHOR), Liu, Yongjing2 (AUTHOR), Li, Hao2 (AUTHOR), Pu, Zhien3 (AUTHOR), Li, Wei3 (AUTHOR), Jiang, Qiantao1,2 (AUTHOR), Ma, Jian1,2 (AUTHOR), Kang, Houyang1,2 (AUTHOR), Qi, Pengfei1,2 (AUTHOR), Xu, Qiang1,2 (AUTHOR), Zhang, Yazhou1,2 (AUTHOR), Deng, Mei1,2 (AUTHOR), Wei, Yuming1,2 (AUTHOR) ymwei@sicau.edu.cn, Chen, Guoyue1,2 (AUTHOR) gychen@sicau.edu.cn, Jiang, Yunfeng1,2 (AUTHOR) jiangyunfeng@sicau.edu.cn
Source: Theoretical & Applied Genetics. Jun2025, Vol. 138 Issue 6, p1-12. 12p.
Abstract: Key message: Three stable QTLs for adult-plant resistance to stripe rust were identified on chromosomes 2DL, 5B, and 7BS in Yunnan hulled wheat, providing durable high-level resistance through additive effects. Yunnan hulled wheat, a unique subspecies native to China, exhibits excellent adult-plant resistance (APR) to stripe rust. However, the genetic basis of stripe rust resistance in Yunnan hulled wheat remains largely unexplored. In this study, we investigated an accession from Lancang County, which has shown stable APR under field conditions for over 10 years. To identify the stripe rust gene of Lancang hulled wheat (LC), we developed 108 F5:7 recombinant inbred lines (RILs) from the cross LC × Avocet S, followed by multi-environment phenotypic evaluation of APR to stripe rust. Bulked segregant exome capture sequencing and quantitative trait locus (QTL) analysis identified three stable QTLs on chromosomes 2DL (QYr.LC-2DL), 5B (QYr.LC-5B), and 7BS (QYr.LC-7BS), explaining 11.44–15.74%, 11.27–15.31%, and 12.12–20.00% of the phenotypic variance, respectively. To validate these QTLs, we constructed three independent heterogeneous inbred families (HIF) segregating for QYr.LC-2DL, QYr.LC-5B, and QYr.LC-7BS from the RIL populations using QTL-linked molecular markers. Further molecular mapping of these three HIF populations refined the localization of QYr.LC-2DL, QYr.LC-5B, and QYr.LC-7BS to intervals between markers KP2D-617.34 and KP2D-625.65, KP5B-121.75 and KP5B-262.42, and KP7B-52.54 and KP7B-58.54, respectively. Additionally, additive effects were observed for QYr.LC-2DL, QYr.LC-5B, and QYr.LC-7BS, with lines carrying all three loci showing the highest resistance. Moreover, the linked markers of QYr.LC-2DL and QYr.LC-7BS can be effectively utilized for marker-assisted selection in wheat breeding programs. These findings provide valuable insights into the genetic basis of stripe rust resistance in Yunnan hulled wheat and offer germplasm for breeding wheat varieties with durable resistance. [ABSTRACT FROM AUTHOR]
Copyright of Theoretical & Applied Genetics is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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  Data: Molecular characterization and validation of adult-plant stripe rust resistance genes in Yunnan hulled wheat (Triticum aestivum ssp. yunnanense King)
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  Data: <searchLink fieldCode="AR" term="%22Huang%2C+Linyu%22">Huang, Linyu</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Wang%2C+Xinyu%22">Wang, Xinyu</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Yan%2C+Yan%22">Yan, Yan</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Long%2C+Li%22">Long, Li</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Li%2C+Qiushuo%22">Li, Qiushuo</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Wang%2C+Yuexue%22">Wang, Yuexue</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Liu%2C+Yongjing%22">Liu, Yongjing</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Li%2C+Hao%22">Li, Hao</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Pu%2C+Zhien%22">Pu, Zhien</searchLink><relatesTo>3</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Li%2C+Wei%22">Li, Wei</searchLink><relatesTo>3</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Jiang%2C+Qiantao%22">Jiang, Qiantao</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Ma%2C+Jian%22">Ma, Jian</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Kang%2C+Houyang%22">Kang, Houyang</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Qi%2C+Pengfei%22">Qi, Pengfei</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Xu%2C+Qiang%22">Xu, Qiang</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Zhang%2C+Yazhou%22">Zhang, Yazhou</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Deng%2C+Mei%22">Deng, Mei</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Wei%2C+Yuming%22">Wei, Yuming</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<i> ymwei@sicau.edu.cn</i><br /><searchLink fieldCode="AR" term="%22Chen%2C+Guoyue%22">Chen, Guoyue</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<i> gychen@sicau.edu.cn</i><br /><searchLink fieldCode="AR" term="%22Jiang%2C+Yunfeng%22">Jiang, Yunfeng</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<i> jiangyunfeng@sicau.edu.cn</i>
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  Data: <searchLink fieldCode="JN" term="%22Theoretical+%26+Applied+Genetics%22">Theoretical & Applied Genetics</searchLink>. Jun2025, Vol. 138 Issue 6, p1-12. 12p.
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Key message: Three stable QTLs for adult-plant resistance to stripe rust were identified on chromosomes 2DL, 5B, and 7BS in Yunnan hulled wheat, providing durable high-level resistance through additive effects. Yunnan hulled wheat, a unique subspecies native to China, exhibits excellent adult-plant resistance (APR) to stripe rust. However, the genetic basis of stripe rust resistance in Yunnan hulled wheat remains largely unexplored. In this study, we investigated an accession from Lancang County, which has shown stable APR under field conditions for over 10 years. To identify the stripe rust gene of Lancang hulled wheat (LC), we developed 108 F5:7 recombinant inbred lines (RILs) from the cross LC × Avocet S, followed by multi-environment phenotypic evaluation of APR to stripe rust. Bulked segregant exome capture sequencing and quantitative trait locus (QTL) analysis identified three stable QTLs on chromosomes 2DL (QYr.LC-2DL), 5B (QYr.LC-5B), and 7BS (QYr.LC-7BS), explaining 11.44–15.74%, 11.27–15.31%, and 12.12–20.00% of the phenotypic variance, respectively. To validate these QTLs, we constructed three independent heterogeneous inbred families (HIF) segregating for QYr.LC-2DL, QYr.LC-5B, and QYr.LC-7BS from the RIL populations using QTL-linked molecular markers. Further molecular mapping of these three HIF populations refined the localization of QYr.LC-2DL, QYr.LC-5B, and QYr.LC-7BS to intervals between markers KP2D-617.34 and KP2D-625.65, KP5B-121.75 and KP5B-262.42, and KP7B-52.54 and KP7B-58.54, respectively. Additionally, additive effects were observed for QYr.LC-2DL, QYr.LC-5B, and QYr.LC-7BS, with lines carrying all three loci showing the highest resistance. Moreover, the linked markers of QYr.LC-2DL and QYr.LC-7BS can be effectively utilized for marker-assisted selection in wheat breeding programs. These findings provide valuable insights into the genetic basis of stripe rust resistance in Yunnan hulled wheat and offer germplasm for breeding wheat varieties with durable resistance. [ABSTRACT FROM AUTHOR]
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  Data: <i>Copyright of Theoretical & Applied Genetics is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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