Elucidating the anticancerous efficacy of genistein via modulating HPV (E7 and E6) oncogenes expression and apoptotic induction in cervical cancer cells.
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| Title: | Elucidating the anticancerous efficacy of genistein via modulating HPV (E7 and E6) oncogenes expression and apoptotic induction in cervical cancer cells. |
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| Authors: | Pandey, Pratibha1,2 (AUTHOR) shukla.pratibha1985@gmail.com, Ramniwas, Seema3 (AUTHOR), Pandey, Shivam4 (AUTHOR), Lakhanpal, Sorabh5 (AUTHOR), Ballal, Suhas6 (AUTHOR), Kumar, Sanjay7 (AUTHOR), Bhat, Mahakshit8 (AUTHOR), Sharma, Shilpa9 (AUTHOR), Kumar, M. Ravi10 (AUTHOR), Khan, Fahad11 (AUTHOR) fahadintegralian@gmail.com |
| Source: | Biotechnology & Applied Biochemistry. Jun2025, Vol. 72 Issue 3, p709-717. 9p. |
| Subjects: | Tumor suppressor proteins, Apoptosis, Gene expression, Etiology of cancer, HeLa cells |
| Abstract: | In recent years, genistein has garnered increased interest for its ability to inhibit numerous deregulated targets associated with cancer progression and induction of programmed cell death and antiproliferative activities in human carcinoma cells. Cancer etiology is influenced via multiple disrupted signaling pathways. This study therefore directed toward investigating genistein efficacy in modulating mRNA expression levels of two crucial Human Pappiloma Virus (HPV) (E7 and E6) oncogenes for cancer treatment. Moreover, the inhibitory effects of genistein for HPV (E7 and E6) oncogenes in cervical carcinoma have not yet been reported. Current study investigated inhibitory potential of genistein in HPV (E7 and E6) oncogenes in HeLa cells. These oncogenes are known to deactivate many tumor suppressor proteins (p53 and pRB). Genistein therapy resulted in decreased cell proliferation and increased cell accumulation in the G (G0/G1) phase in HeLa cell lines. In addition, genistein therapy has resulted in the suppression of HPV (E7 and E6) gene expression and simultaneously increasing expression levels of p53 and pRB mRNA levels. As a consequence, there has been an activation of a series of caspases (3, 8, and 9), resulting in their cleavage. Consequently, our data suggests that genistein could be a powerful candidate for treating cervical cancer by targeting two important oncogenes involved in viral development. However, more in vitro research on primary cervical cancer cells is required to validate the clinically relevant efficacy of genistein against cervical cancer. [ABSTRACT FROM AUTHOR] |
| Copyright of Biotechnology & Applied Biochemistry is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
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| Header | DbId: egs DbLabel: Engineering Source An: 185839101 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Elucidating the anticancerous efficacy of genistein via modulating HPV (E7 and E6) oncogenes expression and apoptotic induction in cervical cancer cells. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Pandey%2C+Pratibha%22">Pandey, Pratibha</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<i> shukla.pratibha1985@gmail.com</i><br /><searchLink fieldCode="AR" term="%22Ramniwas%2C+Seema%22">Ramniwas, Seema</searchLink><relatesTo>3</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Pandey%2C+Shivam%22">Pandey, Shivam</searchLink><relatesTo>4</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Lakhanpal%2C+Sorabh%22">Lakhanpal, Sorabh</searchLink><relatesTo>5</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Ballal%2C+Suhas%22">Ballal, Suhas</searchLink><relatesTo>6</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Kumar%2C+Sanjay%22">Kumar, Sanjay</searchLink><relatesTo>7</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Bhat%2C+Mahakshit%22">Bhat, Mahakshit</searchLink><relatesTo>8</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Sharma%2C+Shilpa%22">Sharma, Shilpa</searchLink><relatesTo>9</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Kumar%2C+M%2E+Ravi%22">Kumar, M. Ravi</searchLink><relatesTo>10</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Khan%2C+Fahad%22">Khan, Fahad</searchLink><relatesTo>11</relatesTo> (AUTHOR)<i> fahadintegralian@gmail.com</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Biotechnology+%26+Applied+Biochemistry%22">Biotechnology & Applied Biochemistry</searchLink>. Jun2025, Vol. 72 Issue 3, p709-717. 9p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Tumor+suppressor+proteins%22">Tumor suppressor proteins</searchLink><br /><searchLink fieldCode="DE" term="%22Apoptosis%22">Apoptosis</searchLink><br /><searchLink fieldCode="DE" term="%22Gene+expression%22">Gene expression</searchLink><br /><searchLink fieldCode="DE" term="%22Etiology+of+cancer%22">Etiology of cancer</searchLink><br /><searchLink fieldCode="DE" term="%22HeLa+cells%22">HeLa cells</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: In recent years, genistein has garnered increased interest for its ability to inhibit numerous deregulated targets associated with cancer progression and induction of programmed cell death and antiproliferative activities in human carcinoma cells. Cancer etiology is influenced via multiple disrupted signaling pathways. This study therefore directed toward investigating genistein efficacy in modulating mRNA expression levels of two crucial Human Pappiloma Virus (HPV) (E7 and E6) oncogenes for cancer treatment. Moreover, the inhibitory effects of genistein for HPV (E7 and E6) oncogenes in cervical carcinoma have not yet been reported. Current study investigated inhibitory potential of genistein in HPV (E7 and E6) oncogenes in HeLa cells. These oncogenes are known to deactivate many tumor suppressor proteins (p53 and pRB). Genistein therapy resulted in decreased cell proliferation and increased cell accumulation in the G (G0/G1) phase in HeLa cell lines. In addition, genistein therapy has resulted in the suppression of HPV (E7 and E6) gene expression and simultaneously increasing expression levels of p53 and pRB mRNA levels. As a consequence, there has been an activation of a series of caspases (3, 8, and 9), resulting in their cleavage. Consequently, our data suggests that genistein could be a powerful candidate for treating cervical cancer by targeting two important oncogenes involved in viral development. However, more in vitro research on primary cervical cancer cells is required to validate the clinically relevant efficacy of genistein against cervical cancer. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Biotechnology & Applied Biochemistry is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1002/bab.2691 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 9 StartPage: 709 Subjects: – SubjectFull: Tumor suppressor proteins Type: general – SubjectFull: Apoptosis Type: general – SubjectFull: Gene expression Type: general – SubjectFull: Etiology of cancer Type: general – SubjectFull: HeLa cells Type: general Titles: – TitleFull: Elucidating the anticancerous efficacy of genistein via modulating HPV (E7 and E6) oncogenes expression and apoptotic induction in cervical cancer cells. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Pandey, Pratibha – PersonEntity: Name: NameFull: Ramniwas, Seema – PersonEntity: Name: NameFull: Pandey, Shivam – PersonEntity: Name: NameFull: Lakhanpal, Sorabh – PersonEntity: Name: NameFull: Ballal, Suhas – PersonEntity: Name: NameFull: Kumar, Sanjay – PersonEntity: Name: NameFull: Bhat, Mahakshit – PersonEntity: Name: NameFull: Sharma, Shilpa – PersonEntity: Name: NameFull: Kumar, M. Ravi – PersonEntity: Name: NameFull: Khan, Fahad IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 06 Text: Jun2025 Type: published Y: 2025 Identifiers: – Type: issn-print Value: 08854513 Numbering: – Type: volume Value: 72 – Type: issue Value: 3 Titles: – TitleFull: Biotechnology & Applied Biochemistry Type: main |
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