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Target-induced DNAzyme recycle amplification strategy for colorimetric detection of cystatin C.

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Title: Target-induced DNAzyme recycle amplification strategy for colorimetric detection of cystatin C.
Authors: Lin, Pin-Yu1 (AUTHOR), Wang, Yi-Shan1,2 (AUTHOR), Ip, Im-Fong1 (AUTHOR), Chang, Chia-Chen1,2,3 (AUTHOR) chang@cgu.edu.tw
Source: Microchemical Journal. Jul2025, Vol. 214, pN.PAG-N.PAG. 1p.
Subjects: Gold nanoparticles, Cystatin C, Nucleic acid hybridization, Acute kidney failure, Deoxyribozymes, Creatinine
Abstract: [Display omitted] • A dual DNAzyme-amplified assay was developed for the detection of cystatin C. • Non-cross-linking gold nanoparticle aggregates were used to induce color signals. • The aptasensor demonstrated high selectivity for detecting cystatin C over other common kidney biomarkers. • The aptasensor effectively detected cystatin C in the serum sample. Cystatin C (Cys C) is an early biomarker of declining kidney function and is frequently detected during clinical diagnosis. However, existing assays are complex, lengthy, and expensive. To overcome these limitations, a dual DNAzyme-amplified assay for Cys C detection was developed using non-cross-linked aggregates of gold nanoparticles (AuNPs). Target-induced assembly of the two DNAzymes triggers a cascade of cleavage reactions, releasing complementary strands that hybridize with detection probes linked to the AuNPs, leading to enhanced plasmonic signals. The assay demonstrated a linear detection range of 2–32 ng/mL, with a detection limit of 1.1 ng/mL. Furthermore, the proposed method exhibited high specificity for Cys C detection, acceptable intra-assay precision, and robust stability. The assay was successfully applied to detect Cys C in serum samples, highlighting its potential for application in the diagnosis of acute kidney injury. The entire process, including aptamer biorecognition, DNA hybridization, and catalytic cleavage of DNAzymes, was performed in a homogeneous solution, thereby rendering the method highly straightforward and exhibiting high reproducibility. [ABSTRACT FROM AUTHOR]
Copyright of Microchemical Journal is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
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DbLabel: Engineering Source
An: 185873873
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PubType: Academic Journal
PubTypeId: academicJournal
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  Data: Target-induced DNAzyme recycle amplification strategy for colorimetric detection of cystatin C.
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  Data: <searchLink fieldCode="AR" term="%22Lin%2C+Pin-Yu%22">Lin, Pin-Yu</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Wang%2C+Yi-Shan%22">Wang, Yi-Shan</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Ip%2C+Im-Fong%22">Ip, Im-Fong</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Chang%2C+Chia-Chen%22">Chang, Chia-Chen</searchLink><relatesTo>1,2,3</relatesTo> (AUTHOR)<i> chang@cgu.edu.tw</i>
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  Data: <searchLink fieldCode="JN" term="%22Microchemical+Journal%22">Microchemical Journal</searchLink>. Jul2025, Vol. 214, pN.PAG-N.PAG. 1p.
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  Data: <searchLink fieldCode="DE" term="%22Gold+nanoparticles%22">Gold nanoparticles</searchLink><br /><searchLink fieldCode="DE" term="%22Cystatin+C%22">Cystatin C</searchLink><br /><searchLink fieldCode="DE" term="%22Nucleic+acid+hybridization%22">Nucleic acid hybridization</searchLink><br /><searchLink fieldCode="DE" term="%22Acute+kidney+failure%22">Acute kidney failure</searchLink><br /><searchLink fieldCode="DE" term="%22Deoxyribozymes%22">Deoxyribozymes</searchLink><br /><searchLink fieldCode="DE" term="%22Creatinine%22">Creatinine</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: [Display omitted] • A dual DNAzyme-amplified assay was developed for the detection of cystatin C. • Non-cross-linking gold nanoparticle aggregates were used to induce color signals. • The aptasensor demonstrated high selectivity for detecting cystatin C over other common kidney biomarkers. • The aptasensor effectively detected cystatin C in the serum sample. Cystatin C (Cys C) is an early biomarker of declining kidney function and is frequently detected during clinical diagnosis. However, existing assays are complex, lengthy, and expensive. To overcome these limitations, a dual DNAzyme-amplified assay for Cys C detection was developed using non-cross-linked aggregates of gold nanoparticles (AuNPs). Target-induced assembly of the two DNAzymes triggers a cascade of cleavage reactions, releasing complementary strands that hybridize with detection probes linked to the AuNPs, leading to enhanced plasmonic signals. The assay demonstrated a linear detection range of 2–32 ng/mL, with a detection limit of 1.1 ng/mL. Furthermore, the proposed method exhibited high specificity for Cys C detection, acceptable intra-assay precision, and robust stability. The assay was successfully applied to detect Cys C in serum samples, highlighting its potential for application in the diagnosis of acute kidney injury. The entire process, including aptamer biorecognition, DNA hybridization, and catalytic cleavage of DNAzymes, was performed in a homogeneous solution, thereby rendering the method highly straightforward and exhibiting high reproducibility. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Microchemical Journal is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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RecordInfo BibRecord:
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      – Type: doi
        Value: 10.1016/j.microc.2025.114039
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      – Code: eng
        Text: English
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        PageCount: 1
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      – SubjectFull: Gold nanoparticles
        Type: general
      – SubjectFull: Cystatin C
        Type: general
      – SubjectFull: Nucleic acid hybridization
        Type: general
      – SubjectFull: Acute kidney failure
        Type: general
      – SubjectFull: Deoxyribozymes
        Type: general
      – SubjectFull: Creatinine
        Type: general
    Titles:
      – TitleFull: Target-induced DNAzyme recycle amplification strategy for colorimetric detection of cystatin C.
        Type: main
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            NameFull: Lin, Pin-Yu
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            NameFull: Wang, Yi-Shan
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            NameFull: Ip, Im-Fong
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            NameFull: Chang, Chia-Chen
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            – D: 01
              M: 07
              Text: Jul2025
              Type: published
              Y: 2025
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              Value: 214
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