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Fluorescence origami paper–based analytical device based on strand displacement assay for SARS-CoV-2 cDNA detection.

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Title: Fluorescence origami paper–based analytical device based on strand displacement assay for SARS-CoV-2 cDNA detection.
Authors: Naorungroj, Sarida1 (AUTHOR), Mahardika, Ignasia Handipta2 (AUTHOR), Huh, Eunjin2 (AUTHOR), Yukird, Jutiporn2 (AUTHOR), Pasomsub, Ekawat3 (AUTHOR), Chailapakul, Orawon1 (AUTHOR) orawon.c@chula.ac.th, Shin, Kwanwoo2 (AUTHOR) kwshin@sogang.ac.kr
Source: Microchimica Acta. Jun2025, Vol. 192 Issue 6, p1-12. 12p.
Subjects: SARS-CoV-2, Complementary DNA, Pseudopotential method, Detection limit, COVID-19 pandemic
Abstract: A fluorescence origami paper–based analytical device (Flu-oPAD) based on a strand displacement assay as a point-of-care testing (POCT) sensing platform for DNA detection is presented. This device facilitates multiple steps in a single device, including sample loading, incubation, and washing. The detection zone was immobilized with a 6-FAM-modified probe (F-probe), which formed a complex with a BHQ1-modified probe (Q-probe) to minimize background signals. In the presence of target DNA, hybridization with the F-probe releases the Q-probe, resulting in a fluorescence response proportional to the target DNA concentration. The sensor exhibits good selectivity for target DNA, with a linearity range from 0.1 nM to 10 μM and an experimental detection limit of 0.1 nM, completing all procedures within 15 min. The real-world applicability is demonstrated by successfully detecting complementary DNA (cDNA) from the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), a cause of the COVID-19 pandemic. This study further introduces a multi-array Flu-oPAD for simultaneous detection of SARS-CoV-2 ORF1ab, N, and E gene cDNAs. This multi-array Flu-oPAD is applied to nasopharyngeal swab samples, showing good agreement with the standard RT-PCR method. Overall, the developed Flu-oPAD has shown great potential as an effective POCT tool for DNA screening. It offers simplicity, portability, accessibility, and cost-effectiveness, offering its potential impact on addressing pressing healthcare needs. [ABSTRACT FROM AUTHOR]
Copyright of Microchimica Acta is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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  Data: Fluorescence origami paper–based analytical device based on strand displacement assay for SARS-CoV-2 cDNA detection.
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  Data: <searchLink fieldCode="AR" term="%22Naorungroj%2C+Sarida%22">Naorungroj, Sarida</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Mahardika%2C+Ignasia+Handipta%22">Mahardika, Ignasia Handipta</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Huh%2C+Eunjin%22">Huh, Eunjin</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Yukird%2C+Jutiporn%22">Yukird, Jutiporn</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Pasomsub%2C+Ekawat%22">Pasomsub, Ekawat</searchLink><relatesTo>3</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Chailapakul%2C+Orawon%22">Chailapakul, Orawon</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> orawon.c@chula.ac.th</i><br /><searchLink fieldCode="AR" term="%22Shin%2C+Kwanwoo%22">Shin, Kwanwoo</searchLink><relatesTo>2</relatesTo> (AUTHOR)<i> kwshin@sogang.ac.kr</i>
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  Data: <searchLink fieldCode="JN" term="%22Microchimica+Acta%22">Microchimica Acta</searchLink>. Jun2025, Vol. 192 Issue 6, p1-12. 12p.
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  Data: <searchLink fieldCode="DE" term="%22SARS-CoV-2%22">SARS-CoV-2</searchLink><br /><searchLink fieldCode="DE" term="%22Complementary+DNA%22">Complementary DNA</searchLink><br /><searchLink fieldCode="DE" term="%22Pseudopotential+method%22">Pseudopotential method</searchLink><br /><searchLink fieldCode="DE" term="%22Detection+limit%22">Detection limit</searchLink><br /><searchLink fieldCode="DE" term="%22COVID-19+pandemic%22">COVID-19 pandemic</searchLink>
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  Label: Abstract
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  Data: A fluorescence origami paper–based analytical device (Flu-oPAD) based on a strand displacement assay as a point-of-care testing (POCT) sensing platform for DNA detection is presented. This device facilitates multiple steps in a single device, including sample loading, incubation, and washing. The detection zone was immobilized with a 6-FAM-modified probe (F-probe), which formed a complex with a BHQ1-modified probe (Q-probe) to minimize background signals. In the presence of target DNA, hybridization with the F-probe releases the Q-probe, resulting in a fluorescence response proportional to the target DNA concentration. The sensor exhibits good selectivity for target DNA, with a linearity range from 0.1 nM to 10 μM and an experimental detection limit of 0.1 nM, completing all procedures within 15 min. The real-world applicability is demonstrated by successfully detecting complementary DNA (cDNA) from the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), a cause of the COVID-19 pandemic. This study further introduces a multi-array Flu-oPAD for simultaneous detection of SARS-CoV-2 ORF1ab, N, and E gene cDNAs. This multi-array Flu-oPAD is applied to nasopharyngeal swab samples, showing good agreement with the standard RT-PCR method. Overall, the developed Flu-oPAD has shown great potential as an effective POCT tool for DNA screening. It offers simplicity, portability, accessibility, and cost-effectiveness, offering its potential impact on addressing pressing healthcare needs. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
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  Data: <i>Copyright of Microchimica Acta is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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      – SubjectFull: Pseudopotential method
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      – SubjectFull: Detection limit
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              M: 06
              Text: Jun2025
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              Y: 2025
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