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Target-triggered hybrid chain amplified fluorescence aptasensor based on label-free dye and MnO2 nanosheets system for Escherichia coli detection.

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Title: Target-triggered hybrid chain amplified fluorescence aptasensor based on label-free dye and MnO2 nanosheets system for Escherichia coli detection.
Authors: Liang, Hao1 (AUTHOR), Li, Danliang2 (AUTHOR), Jia, Shiyu1 (AUTHOR), Zhang, Xuebing1 (AUTHOR), Bai, Qinqin1 (AUTHOR), Wang, Ziyi1 (AUTHOR), Cai, Yujiao1 (AUTHOR), Liu, Jian1 (AUTHOR), Chen, Lili1 (AUTHOR) chlili720612@163.com
Source: Microchimica Acta. Jun2025, Vol. 192 Issue 6, p1-10. 10p.
Subjects: Escherichia coli, Single-stranded DNA, Bacterial contamination, Complex matrices, Food safety
Abstract: Bacterial contamination poses significant threats to public health through food safety issues, creating a critical need for rapid and sensitive bacterial detection platforms. Herein, we developed a novel label-free fluorescent detection system leveraging target-triggered hybridization chain reaction (HCR) amplification, using Escherichia coli (E. coli) as a proof of concept. We engineered a hairpin-structured capture probe integrating an E. coli-specific aptamer with an HCR trigger sequence, achieving fluorescence amplification through three synergistic mechanisms: (1) HCR-driven DNA duplex assembly; (2) enhanced SYBR Green I (SG-I, a cost-effective non-labeled dye) intercalation into HCR-generated DNA duplexes; and (3) target-selective fluorescence modulation via MnO2 nanosheets that quench background fluorescence through preferential adsorption of single-stranded DNA (ssDNA)-bound SG-I over duplex DNA-intercalated dye, exploiting the differential binding affinity of MnO2 for ssDNA versus double-stranded DNA. This strategy enabled a fluorescence sensor with high sensitivity (detection limit: 17 CFU/mL), excellent specificity, and broad dynamic range (5.0 × 101 ~ 5.0 × 107 CFU/mL), demonstrating robust performance in complex food matrices through successful E. coli detection in spiked milk and lettuce samples with recoveries of 98.81 to 104.26%, thereby underscoring the method's reliability and practical utility for on-site food safety monitoring in real-world scenarios. [ABSTRACT FROM AUTHOR]
Copyright of Microchimica Acta is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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  Data: Target-triggered hybrid chain amplified fluorescence aptasensor based on label-free dye and MnO<subscript>2</subscript> nanosheets system for Escherichia coli detection.
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  Data: <searchLink fieldCode="DE" term="%22Escherichia+coli%22">Escherichia coli</searchLink><br /><searchLink fieldCode="DE" term="%22Single-stranded+DNA%22">Single-stranded DNA</searchLink><br /><searchLink fieldCode="DE" term="%22Bacterial+contamination%22">Bacterial contamination</searchLink><br /><searchLink fieldCode="DE" term="%22Complex+matrices%22">Complex matrices</searchLink><br /><searchLink fieldCode="DE" term="%22Food+safety%22">Food safety</searchLink>
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  Data: Bacterial contamination poses significant threats to public health through food safety issues, creating a critical need for rapid and sensitive bacterial detection platforms. Herein, we developed a novel label-free fluorescent detection system leveraging target-triggered hybridization chain reaction (HCR) amplification, using Escherichia coli (E. coli) as a proof of concept. We engineered a hairpin-structured capture probe integrating an E. coli-specific aptamer with an HCR trigger sequence, achieving fluorescence amplification through three synergistic mechanisms: (1) HCR-driven DNA duplex assembly; (2) enhanced SYBR Green I (SG-I, a cost-effective non-labeled dye) intercalation into HCR-generated DNA duplexes; and (3) target-selective fluorescence modulation via MnO2 nanosheets that quench background fluorescence through preferential adsorption of single-stranded DNA (ssDNA)-bound SG-I over duplex DNA-intercalated dye, exploiting the differential binding affinity of MnO2 for ssDNA versus double-stranded DNA. This strategy enabled a fluorescence sensor with high sensitivity (detection limit: 17 CFU/mL), excellent specificity, and broad dynamic range (5.0 × 101 ~ 5.0 × 107 CFU/mL), demonstrating robust performance in complex food matrices through successful E. coli detection in spiked milk and lettuce samples with recoveries of 98.81 to 104.26%, thereby underscoring the method's reliability and practical utility for on-site food safety monitoring in real-world scenarios. [ABSTRACT FROM AUTHOR]
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  Label:
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  Data: <i>Copyright of Microchimica Acta is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Value: 10.1007/s00604-025-07244-4
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      – SubjectFull: Single-stranded DNA
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      – SubjectFull: Bacterial contamination
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      – SubjectFull: Complex matrices
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      – SubjectFull: Food safety
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      – TitleFull: Target-triggered hybrid chain amplified fluorescence aptasensor based on label-free dye and MnO2 nanosheets system for Escherichia coli detection.
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            NameFull: Liang, Hao
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            NameFull: Li, Danliang
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            – D: 01
              M: 06
              Text: Jun2025
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              Y: 2025
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