Target-triggered hybrid chain amplified fluorescence aptasensor based on label-free dye and MnO2 nanosheets system for Escherichia coli detection.
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| Title: | Target-triggered hybrid chain amplified fluorescence aptasensor based on label-free dye and MnO |
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| Authors: | Liang, Hao1 (AUTHOR), Li, Danliang2 (AUTHOR), Jia, Shiyu1 (AUTHOR), Zhang, Xuebing1 (AUTHOR), Bai, Qinqin1 (AUTHOR), Wang, Ziyi1 (AUTHOR), Cai, Yujiao1 (AUTHOR), Liu, Jian1 (AUTHOR), Chen, Lili1 (AUTHOR) chlili720612@163.com |
| Source: | Microchimica Acta. Jun2025, Vol. 192 Issue 6, p1-10. 10p. |
| Subjects: | Escherichia coli, Single-stranded DNA, Bacterial contamination, Complex matrices, Food safety |
| Abstract: | Bacterial contamination poses significant threats to public health through food safety issues, creating a critical need for rapid and sensitive bacterial detection platforms. Herein, we developed a novel label-free fluorescent detection system leveraging target-triggered hybridization chain reaction (HCR) amplification, using Escherichia coli (E. coli) as a proof of concept. We engineered a hairpin-structured capture probe integrating an E. coli-specific aptamer with an HCR trigger sequence, achieving fluorescence amplification through three synergistic mechanisms: (1) HCR-driven DNA duplex assembly; (2) enhanced SYBR Green I (SG-I, a cost-effective non-labeled dye) intercalation into HCR-generated DNA duplexes; and (3) target-selective fluorescence modulation via MnO2 nanosheets that quench background fluorescence through preferential adsorption of single-stranded DNA (ssDNA)-bound SG-I over duplex DNA-intercalated dye, exploiting the differential binding affinity of MnO2 for ssDNA versus double-stranded DNA. This strategy enabled a fluorescence sensor with high sensitivity (detection limit: 17 CFU/mL), excellent specificity, and broad dynamic range (5.0 × 101 ~ 5.0 × 107 CFU/mL), demonstrating robust performance in complex food matrices through successful E. coli detection in spiked milk and lettuce samples with recoveries of 98.81 to 104.26%, thereby underscoring the method's reliability and practical utility for on-site food safety monitoring in real-world scenarios. [ABSTRACT FROM AUTHOR] |
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| Database: | Engineering Source |
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| Abstract: | Bacterial contamination poses significant threats to public health through food safety issues, creating a critical need for rapid and sensitive bacterial detection platforms. Herein, we developed a novel label-free fluorescent detection system leveraging target-triggered hybridization chain reaction (HCR) amplification, using Escherichia coli (E. coli) as a proof of concept. We engineered a hairpin-structured capture probe integrating an E. coli-specific aptamer with an HCR trigger sequence, achieving fluorescence amplification through three synergistic mechanisms: (1) HCR-driven DNA duplex assembly; (2) enhanced SYBR Green I (SG-I, a cost-effective non-labeled dye) intercalation into HCR-generated DNA duplexes; and (3) target-selective fluorescence modulation via MnO2 nanosheets that quench background fluorescence through preferential adsorption of single-stranded DNA (ssDNA)-bound SG-I over duplex DNA-intercalated dye, exploiting the differential binding affinity of MnO2 for ssDNA versus double-stranded DNA. This strategy enabled a fluorescence sensor with high sensitivity (detection limit: 17 CFU/mL), excellent specificity, and broad dynamic range (5.0 × 101 ~ 5.0 × 107 CFU/mL), demonstrating robust performance in complex food matrices through successful E. coli detection in spiked milk and lettuce samples with recoveries of 98.81 to 104.26%, thereby underscoring the method's reliability and practical utility for on-site food safety monitoring in real-world scenarios. [ABSTRACT FROM AUTHOR] |
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| ISSN: | 00263672 |
| DOI: | 10.1007/s00604-025-07244-4 |
