A unique architecture of an engineered amphiphilic chemotherapeutic homopolymer enables fluorescence-assisted intracellular tracking and pH responsive drug delivery.
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| Title: | A unique architecture of an engineered amphiphilic chemotherapeutic homopolymer enables fluorescence-assisted intracellular tracking and pH responsive drug delivery. |
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| Authors: | Kumar, Pawan1 (AUTHOR) pkiiserk@gmail.com, Patra, Diptendu1 (AUTHOR), Shunmugam, Raja1 (AUTHOR) sraja@iiserkol.ac.in |
| Source: | Journal of Macromolecular Science: Pure & Applied Chemistry. 2025, Vol. 62 Issue 6, p493-499. 7p. |
| Subjects: | Intracellular tracking, Transmission electron microscopes, Alkylating agents, Ethylene glycol, Cyclopolymerization, Glycols |
| Abstract: | Tracking of non-fluorescent drug after internalization is one of the major challenges. Herein, we developed homopolymer system where fluorescent probe and non-fluorescent drug are attached with similar pH-responsive linker. Alkylating agent drugs chlorambucil and fluorophore 1-pyrenebutyric acid were used as model molecules. Monomethyl poly(ethylene glycol) (mPeg-OH) was used for water solubility. Diacetylene moiety is well known for its cyclopolymerization. Towards this, DIOL containing diacetylene moiety was synthesized and selectively, one of the -OH group of DIOL was attached to water soluble moiety, whereas another -OH group was attached to free carboxylic acid-functionalized moiety containing chlorambucil and 1-pyrenebutyric acid, resulting in macromonomer MPPDC. Further, MPPDC was polymerized by Hoveyda Grubbs' second generation (HG2) catalyst to get our final amphiphilic polymer PMPPDC. All the molecules were characterized carefully by different analytical techniques. Size and morphology were studied using dynamic light scattering (DLS) and transmission electron microscope (TEM), respectively, followed by release study at different pH. Cell viability and internalization studies were carried out with HeLa and MCF 7 cell lines. PMPPDC showed good cell growth inhibition in both cell lines. Similarly, increase in internalization was observed with increase in concentration of polymer PMPPDC in epifluorescence image. [ABSTRACT FROM AUTHOR] |
| Copyright of Journal of Macromolecular Science: Pure & Applied Chemistry is the property of Taylor & Francis Ltd and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
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| Header | DbId: egs DbLabel: Engineering Source An: 186130814 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: A unique architecture of an engineered amphiphilic chemotherapeutic homopolymer enables fluorescence-assisted intracellular tracking and pH responsive drug delivery. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Kumar%2C+Pawan%22">Kumar, Pawan</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> pkiiserk@gmail.com</i><br /><searchLink fieldCode="AR" term="%22Patra%2C+Diptendu%22">Patra, Diptendu</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Shunmugam%2C+Raja%22">Shunmugam, Raja</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> sraja@iiserkol.ac.in</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Journal+of+Macromolecular+Science%3A+Pure+%26+Applied+Chemistry%22">Journal of Macromolecular Science: Pure & Applied Chemistry</searchLink>. 2025, Vol. 62 Issue 6, p493-499. 7p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Intracellular+tracking%22">Intracellular tracking</searchLink><br /><searchLink fieldCode="DE" term="%22Transmission+electron+microscopes%22">Transmission electron microscopes</searchLink><br /><searchLink fieldCode="DE" term="%22Alkylating+agents%22">Alkylating agents</searchLink><br /><searchLink fieldCode="DE" term="%22Ethylene+glycol%22">Ethylene glycol</searchLink><br /><searchLink fieldCode="DE" term="%22Cyclopolymerization%22">Cyclopolymerization</searchLink><br /><searchLink fieldCode="DE" term="%22Glycols%22">Glycols</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Tracking of non-fluorescent drug after internalization is one of the major challenges. Herein, we developed homopolymer system where fluorescent probe and non-fluorescent drug are attached with similar pH-responsive linker. Alkylating agent drugs chlorambucil and fluorophore 1-pyrenebutyric acid were used as model molecules. Monomethyl poly(ethylene glycol) (mPeg-OH) was used for water solubility. Diacetylene moiety is well known for its cyclopolymerization. Towards this, DIOL containing diacetylene moiety was synthesized and selectively, one of the -OH group of DIOL was attached to water soluble moiety, whereas another -OH group was attached to free carboxylic acid-functionalized moiety containing chlorambucil and 1-pyrenebutyric acid, resulting in macromonomer MPPDC. Further, MPPDC was polymerized by Hoveyda Grubbs' second generation (HG2) catalyst to get our final amphiphilic polymer PMPPDC. All the molecules were characterized carefully by different analytical techniques. Size and morphology were studied using dynamic light scattering (DLS) and transmission electron microscope (TEM), respectively, followed by release study at different pH. Cell viability and internalization studies were carried out with HeLa and MCF 7 cell lines. PMPPDC showed good cell growth inhibition in both cell lines. Similarly, increase in internalization was observed with increase in concentration of polymer PMPPDC in epifluorescence image. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Journal of Macromolecular Science: Pure & Applied Chemistry is the property of Taylor & Francis Ltd and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1080/10601325.2025.2490087 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 7 StartPage: 493 Subjects: – SubjectFull: Intracellular tracking Type: general – SubjectFull: Transmission electron microscopes Type: general – SubjectFull: Alkylating agents Type: general – SubjectFull: Ethylene glycol Type: general – SubjectFull: Cyclopolymerization Type: general – SubjectFull: Glycols Type: general Titles: – TitleFull: A unique architecture of an engineered amphiphilic chemotherapeutic homopolymer enables fluorescence-assisted intracellular tracking and pH responsive drug delivery. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Kumar, Pawan – PersonEntity: Name: NameFull: Patra, Diptendu – PersonEntity: Name: NameFull: Shunmugam, Raja IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 06 Text: 2025 Type: published Y: 2025 Identifiers: – Type: issn-print Value: 10601325 Numbering: – Type: volume Value: 62 – Type: issue Value: 6 Titles: – TitleFull: Journal of Macromolecular Science: Pure & Applied Chemistry Type: main |
| ResultId | 1 |
