Cloning and expression analysis of Mildew Locus O (MLO) genes related to powdery mildew resistance in Vitis pseudoreticulata, and functional characterisation of VpMLO13.
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| Title: | Cloning and expression analysis of Mildew Locus O (MLO) genes related to powdery mildew resistance in Vitis pseudoreticulata, and functional characterisation of VpMLO13. |
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| Authors: | Huang, Congbo1,2 (AUTHOR), Wang, Yanqing1,2 (AUTHOR), Zhang, Xiaoying1,2 (AUTHOR), Zhang, Chaohong1,2 (AUTHOR) zhangchaohong@nwsuaf.edu.cn |
| Source: | New Zealand Journal of Crop & Horticultural Science. Dec2025, Vol. 53 Issue 4, p1031-1047. 17p. |
| Subjects: | Molecular cloning, Functional analysis, Cell membranes, Natural immunity, Arabidopsis thaliana, Powdery mildew diseases, Vitis vinifera |
| Abstract: | In many plants, Mildew Locus O (MLO) genes facilitate infection by pathogens, including Erysiphe necator, the causative agent of powdery mildew (PM) in grapevines. The function of VvMLOs in Vitis vinifera has previously been demonstrated, but the identity and function of VpMLOs in V. pseudoreticulata are not yet clear. Here, we cloned MLO genes that have been implicated in PM susceptibility from the highly PM-resistant V. pseudoreticulata accession 'Baihe-35-1' and the PM-susceptible V. vinifera 'Carinena'. The open reading frames (ORFs) of MLO3, MLO6, and MLO8 differed between 'Baihe-35-1' and 'Carinena'. After inoculation of the leaves with Erysiphe necator, six MLO genes exhibited two distinct expression patterns in 'Baihe-35-1' and 'Carinena'. Consistent with the MLO proteins in other plants, we also found that VpMLO3, VpMLO6, VpMLO11, and VpMLO13 are all localised to the plasma membrane. To further support a role for VpMLOs in susceptibility to PM, we expressed VpMlo13 in the PM-resistant Arabidopsis thaliana MLO2/6/12 triple mutant, which showed that transgenic expression of VpMLO13 restored susceptibility to PM. In addition, a yeast two-hybrid (Y2H) assay showed that VpMLO13 interacted with calmodulin (VpCaM). These results will provide a basis for further investigating the PM resistance mechanisms in V. pseudoreticulata. [ABSTRACT FROM AUTHOR] |
| Copyright of New Zealand Journal of Crop & Horticultural Science is the property of Taylor & Francis Ltd and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
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| Items | – Name: Title Label: Title Group: Ti Data: Cloning and expression analysis of Mildew Locus O (MLO) genes related to powdery mildew resistance in Vitis pseudoreticulata, and functional characterisation of VpMLO13. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Huang%2C+Congbo%22">Huang, Congbo</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Wang%2C+Yanqing%22">Wang, Yanqing</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Zhang%2C+Xiaoying%22">Zhang, Xiaoying</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Zhang%2C+Chaohong%22">Zhang, Chaohong</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<i> zhangchaohong@nwsuaf.edu.cn</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22New+Zealand+Journal+of+Crop+%26+Horticultural+Science%22">New Zealand Journal of Crop & Horticultural Science</searchLink>. Dec2025, Vol. 53 Issue 4, p1031-1047. 17p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Molecular+cloning%22">Molecular cloning</searchLink><br /><searchLink fieldCode="DE" term="%22Functional+analysis%22">Functional analysis</searchLink><br /><searchLink fieldCode="DE" term="%22Cell+membranes%22">Cell membranes</searchLink><br /><searchLink fieldCode="DE" term="%22Natural+immunity%22">Natural immunity</searchLink><br /><searchLink fieldCode="DE" term="%22Arabidopsis+thaliana%22">Arabidopsis thaliana</searchLink><br /><searchLink fieldCode="DE" term="%22Powdery+mildew+diseases%22">Powdery mildew diseases</searchLink><br /><searchLink fieldCode="DE" term="%22Vitis+vinifera%22">Vitis vinifera</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: In many plants, Mildew Locus O (MLO) genes facilitate infection by pathogens, including Erysiphe necator, the causative agent of powdery mildew (PM) in grapevines. The function of VvMLOs in Vitis vinifera has previously been demonstrated, but the identity and function of VpMLOs in V. pseudoreticulata are not yet clear. Here, we cloned MLO genes that have been implicated in PM susceptibility from the highly PM-resistant V. pseudoreticulata accession 'Baihe-35-1' and the PM-susceptible V. vinifera 'Carinena'. The open reading frames (ORFs) of MLO3, MLO6, and MLO8 differed between 'Baihe-35-1' and 'Carinena'. After inoculation of the leaves with Erysiphe necator, six MLO genes exhibited two distinct expression patterns in 'Baihe-35-1' and 'Carinena'. Consistent with the MLO proteins in other plants, we also found that VpMLO3, VpMLO6, VpMLO11, and VpMLO13 are all localised to the plasma membrane. To further support a role for VpMLOs in susceptibility to PM, we expressed VpMlo13 in the PM-resistant Arabidopsis thaliana MLO2/6/12 triple mutant, which showed that transgenic expression of VpMLO13 restored susceptibility to PM. In addition, a yeast two-hybrid (Y2H) assay showed that VpMLO13 interacted with calmodulin (VpCaM). These results will provide a basis for further investigating the PM resistance mechanisms in V. pseudoreticulata. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of New Zealand Journal of Crop & Horticultural Science is the property of Taylor & Francis Ltd and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1080/01140671.2023.2280185 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 17 StartPage: 1031 Subjects: – SubjectFull: Molecular cloning Type: general – SubjectFull: Functional analysis Type: general – SubjectFull: Cell membranes Type: general – SubjectFull: Natural immunity Type: general – SubjectFull: Arabidopsis thaliana Type: general – SubjectFull: Powdery mildew diseases Type: general – SubjectFull: Vitis vinifera Type: general Titles: – TitleFull: Cloning and expression analysis of Mildew Locus O (MLO) genes related to powdery mildew resistance in Vitis pseudoreticulata, and functional characterisation of VpMLO13. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Huang, Congbo – PersonEntity: Name: NameFull: Wang, Yanqing – PersonEntity: Name: NameFull: Zhang, Xiaoying – PersonEntity: Name: NameFull: Zhang, Chaohong IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 12 Text: Dec2025 Type: published Y: 2025 Identifiers: – Type: issn-print Value: 01140671 Numbering: – Type: volume Value: 53 – Type: issue Value: 4 Titles: – TitleFull: New Zealand Journal of Crop & Horticultural Science Type: main |
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