DNA replication during sporulation in Myxococcus xanthus fruiting bodies.
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| Title: | DNA replication during sporulation in Myxococcus xanthus fruiting bodies. |
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| Authors: | Linfong Tzeng1, Singer, Mitchell1 mhsinger@ucdavis.edu |
| Source: | Proceedings of the National Academy of Sciences of the United States of America. 10/4/2005, Vol. 102 Issue 40, p14428-14433. 6p. |
| Subjects: | DNA replication, DNA synthesis, Myxococcus xanthus, Flow cytometry, Cytological techniques, Fluorescence microscopy |
| Abstract: | During the developmental process of the Gram-negative soil bacterium Myxococcus xanthus, vegetatively growing rod cells differentiate to ultimately become metabolically quiescent and environ- mentally resistant myxospores encased within fruiting bodies. This program, initiated by nutrient deprivation, is propagated by both cell-autonomous and cell-nonautonomous signals. Our goal was to determine whether M. xanthus, like many other developmental systems, uses cell-cycle cues to regulate and control its developmental program. To address this question, the DNA replication cycle was used as a marker to monitor progression through the cell cycle in vegetative, stationary, and developing M. xanthus populations. Using flow cytometry, quantitative fluorescence micros-copy, and FISH to establish the chromosome copy number of myxospores, it was determined that vegetatively growing cells contain one to two copies of the genome, but upon entry into stationary phase, the chromosome copy number drops to a single copy. Of particular interest, fruiting body-derived myxospores contain a specific two-chromosome DNA complement with both origin and terminus regions localized to the periphery of the myxospore. We speculate that this duplication of genetic information in the myxospore would help assure viability during germination by providing a second copy of each gene. The results of this study imply that not only is DNA replication tightly regulated during the developmental process of M. xanthus, but that there are also regulatory mechanisms to ensure that all myxospores acquire two copies of the chromosome. [ABSTRACT FROM AUTHOR] |
| Copyright of Proceedings of the National Academy of Sciences of the United States of America is the property of National Academy of Sciences and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Text: Availability: 0 |
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| Header | DbId: egs DbLabel: Engineering Source An: 18631456 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: DNA replication during sporulation in Myxococcus xanthus fruiting bodies. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Linfong+Tzeng%22">Linfong Tzeng</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Singer%2C+Mitchell%22">Singer, Mitchell</searchLink><relatesTo>1</relatesTo><i> mhsinger@ucdavis.edu</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Proceedings+of+the+National+Academy+of+Sciences+of+the+United+States+of+America%22">Proceedings of the National Academy of Sciences of the United States of America</searchLink>. 10/4/2005, Vol. 102 Issue 40, p14428-14433. 6p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22DNA+replication%22">DNA replication</searchLink><br /><searchLink fieldCode="DE" term="%22DNA+synthesis%22">DNA synthesis</searchLink><br /><searchLink fieldCode="DE" term="%22Myxococcus+xanthus%22">Myxococcus xanthus</searchLink><br /><searchLink fieldCode="DE" term="%22Flow+cytometry%22">Flow cytometry</searchLink><br /><searchLink fieldCode="DE" term="%22Cytological+techniques%22">Cytological techniques</searchLink><br /><searchLink fieldCode="DE" term="%22Fluorescence+microscopy%22">Fluorescence microscopy</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: During the developmental process of the Gram-negative soil bacterium Myxococcus xanthus, vegetatively growing rod cells differentiate to ultimately become metabolically quiescent and environ- mentally resistant myxospores encased within fruiting bodies. This program, initiated by nutrient deprivation, is propagated by both cell-autonomous and cell-nonautonomous signals. Our goal was to determine whether M. xanthus, like many other developmental systems, uses cell-cycle cues to regulate and control its developmental program. To address this question, the DNA replication cycle was used as a marker to monitor progression through the cell cycle in vegetative, stationary, and developing M. xanthus populations. Using flow cytometry, quantitative fluorescence micros-copy, and FISH to establish the chromosome copy number of myxospores, it was determined that vegetatively growing cells contain one to two copies of the genome, but upon entry into stationary phase, the chromosome copy number drops to a single copy. Of particular interest, fruiting body-derived myxospores contain a specific two-chromosome DNA complement with both origin and terminus regions localized to the periphery of the myxospore. We speculate that this duplication of genetic information in the myxospore would help assure viability during germination by providing a second copy of each gene. The results of this study imply that not only is DNA replication tightly regulated during the developmental process of M. xanthus, but that there are also regulatory mechanisms to ensure that all myxospores acquire two copies of the chromosome. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Proceedings of the National Academy of Sciences of the United States of America is the property of National Academy of Sciences and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1073/pnas.0506969102 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 6 StartPage: 14428 Subjects: – SubjectFull: DNA replication Type: general – SubjectFull: DNA synthesis Type: general – SubjectFull: Myxococcus xanthus Type: general – SubjectFull: Flow cytometry Type: general – SubjectFull: Cytological techniques Type: general – SubjectFull: Fluorescence microscopy Type: general Titles: – TitleFull: DNA replication during sporulation in Myxococcus xanthus fruiting bodies. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Linfong Tzeng – PersonEntity: Name: NameFull: Singer, Mitchell IsPartOfRelationships: – BibEntity: Dates: – D: 04 M: 10 Text: 10/4/2005 Type: published Y: 2005 Identifiers: – Type: issn-print Value: 00278424 Numbering: – Type: volume Value: 102 – Type: issue Value: 40 Titles: – TitleFull: Proceedings of the National Academy of Sciences of the United States of America Type: main |
| ResultId | 1 |
