Optimization of the Green Conventional Extraction Method of Sericin from Silkworm.
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| Title: | Optimization of the Green Conventional Extraction Method of Sericin from Silkworm. |
|---|---|
| Authors: | Burgos Gomez, Daniel Stiven1 (AUTHOR), Rada-Mendoza, Maite1 (AUTHOR) mrada@unicauca.edu.co, Chito-Trujillo, Diana M.1 (AUTHOR) |
| Source: | Polymers (20734360). Jul2025, Vol. 17 Issue 13, p1823. 14p. |
| Subjects: | Sericin, Silkworms, Biopolymers, Experimental design, Sustainability, Bioengineering, Extraction (Chemistry) |
| Abstract: | In the silk production process, cocoons from Bombyx mori worm are degummed and separated from their components. This step generates large residual quantities of an aqueous solution containing various chemical substances, including sericin—a protein that, when discarded improperly, negatively impacts the environment. Sodium bicarbonate and coconut soap are commonly used in the degumming process. The phosphates in the soap and the sodium bicarbonate increase the biological oxygen demand (BOD) and chemical oxygen demand (COD), leading to water contamination. In this study, a Box–Behnken experimental design was used to maximize the extraction of sericin through a conventional extraction under chemical-free conditions. From a total of 45 experiments, the optimal extraction conditions were identified as a solid-to-liquid ratio of 1:20 w/v, a temperature of 120 °C, and 90 min of extraction time. Sericin yields ranged from 9% to 18%. Infrared spectroscopic characterization of the extracted sericin confirmed the presence of protein-specific functional groups and common interactions associated with β-sheet structures. Fractions of high molecular weight (50 kDa to 200 kDa), identified by means of Sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) analysis, demonstrate the potential functionality of extracted sericin for the development of biopolymer films useful in biomedical and food industry applications. The optimized methodology is a good alternative to recycle the waste of sericulture chain for obtaining extracts enriched in sericin, as well as to promote the mechanization of artisanal production processes. [ABSTRACT FROM AUTHOR] |
| Copyright of Polymers (20734360) is the property of MDPI and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
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| Header | DbId: egs DbLabel: Engineering Source An: 186597737 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Optimization of the Green Conventional Extraction Method of Sericin from Silkworm. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Burgos+Gomez%2C+Daniel+Stiven%22">Burgos Gomez, Daniel Stiven</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Rada-Mendoza%2C+Maite%22">Rada-Mendoza, Maite</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> mrada@unicauca.edu.co</i><br /><searchLink fieldCode="AR" term="%22Chito-Trujillo%2C+Diana+M%2E%22">Chito-Trujillo, Diana M.</searchLink><relatesTo>1</relatesTo> (AUTHOR) – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Polymers+%2820734360%29%22">Polymers (20734360)</searchLink>. Jul2025, Vol. 17 Issue 13, p1823. 14p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Sericin%22">Sericin</searchLink><br /><searchLink fieldCode="DE" term="%22Silkworms%22">Silkworms</searchLink><br /><searchLink fieldCode="DE" term="%22Biopolymers%22">Biopolymers</searchLink><br /><searchLink fieldCode="DE" term="%22Experimental+design%22">Experimental design</searchLink><br /><searchLink fieldCode="DE" term="%22Sustainability%22">Sustainability</searchLink><br /><searchLink fieldCode="DE" term="%22Bioengineering%22">Bioengineering</searchLink><br /><searchLink fieldCode="DE" term="%22Extraction+%28Chemistry%29%22">Extraction (Chemistry)</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: In the silk production process, cocoons from Bombyx mori worm are degummed and separated from their components. This step generates large residual quantities of an aqueous solution containing various chemical substances, including sericin—a protein that, when discarded improperly, negatively impacts the environment. Sodium bicarbonate and coconut soap are commonly used in the degumming process. The phosphates in the soap and the sodium bicarbonate increase the biological oxygen demand (BOD) and chemical oxygen demand (COD), leading to water contamination. In this study, a Box–Behnken experimental design was used to maximize the extraction of sericin through a conventional extraction under chemical-free conditions. From a total of 45 experiments, the optimal extraction conditions were identified as a solid-to-liquid ratio of 1:20 w/v, a temperature of 120 °C, and 90 min of extraction time. Sericin yields ranged from 9% to 18%. Infrared spectroscopic characterization of the extracted sericin confirmed the presence of protein-specific functional groups and common interactions associated with β-sheet structures. Fractions of high molecular weight (50 kDa to 200 kDa), identified by means of Sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) analysis, demonstrate the potential functionality of extracted sericin for the development of biopolymer films useful in biomedical and food industry applications. The optimized methodology is a good alternative to recycle the waste of sericulture chain for obtaining extracts enriched in sericin, as well as to promote the mechanization of artisanal production processes. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Polymers (20734360) is the property of MDPI and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.3390/polym17131823 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 14 StartPage: 1823 Subjects: – SubjectFull: Sericin Type: general – SubjectFull: Silkworms Type: general – SubjectFull: Biopolymers Type: general – SubjectFull: Experimental design Type: general – SubjectFull: Sustainability Type: general – SubjectFull: Bioengineering Type: general – SubjectFull: Extraction (Chemistry) Type: general Titles: – TitleFull: Optimization of the Green Conventional Extraction Method of Sericin from Silkworm. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Burgos Gomez, Daniel Stiven – PersonEntity: Name: NameFull: Rada-Mendoza, Maite – PersonEntity: Name: NameFull: Chito-Trujillo, Diana M. IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 07 Text: Jul2025 Type: published Y: 2025 Identifiers: – Type: issn-print Value: 20734360 Numbering: – Type: volume Value: 17 – Type: issue Value: 13 Titles: – TitleFull: Polymers (20734360) Type: main |
| ResultId | 1 |
