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A step-by-step approach to minimally photothrombotic ischemic stroke in the hippocampal region that simulates human stroke.

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Title: A step-by-step approach to minimally photothrombotic ischemic stroke in the hippocampal region that simulates human stroke.
Authors: Fazli, Farzaneh1 (AUTHOR) farzaneh1219@gmail.com, Tayefi Nasrabadi, Hamid1 (AUTHOR) Tayefih@yahoo.com, Rahbarghazi, Reza1 (AUTHOR) rezarahbardvm@gmail.com, Sadigh‑Eteghad, Saeed1 (AUTHOR) sadighs@tbzmed.ac.ir, Zibaii, Mohammad Ismail2 (AUTHOR) mizibaye@gmail.com, Shimia, Mohammad1 (AUTHOR) mshimia@yahoo.com, Karimipour, Mohammad1 (AUTHOR) karimipourm@tbzmed.ac.ir
Source: Lasers in Medical Science. 7/11/2025, Vol. 40 Issue 1, p1-11. 11p.
Subjects: Rose bengal, Learning ability testing, Microscopy, Nerve tissue, Statistical significance
Abstract: The hippocampal structure, characterized by blood vessels with an average diameter of 0.5 mm, exhibits a significant vulnerability to ischemic conditions and is the critical region in the learning process and memory establishment. Male mice (n = 30) were divided into three groups (each group n = 10). 1) Rose Bengal group, 2) Laser group, 3) Rose Bengal + Laser group. Mice were placed in a stereotaxic device. An optical fiber was positioned above the ascending part of the hippocampal fissure and illuminated for 15 min using a previously injected intraperitoneal Rose Bengal dye. The Passive avoidance and MWM tests evaluated learning ability and memory capacity. TTC and Cresyl violet staining were conducted to assess the neural tissue morphology and histological alterations. Photochemical stroke resulted in hemorrhagic tissue and pale ischemic changes over three days. Microscopic analysis revealed neural tissue degeneration and disruption in the (Rose Bengal + Laser) group compared to the other experimental groups. The passive avoidance test demonstrated a statistically significant reduction in the time required to reach the dark compartment within the (Rose Bengal + Laser) group relative to the other groups (P < 0.001). Furthermore, treatment with Rose Bengal and laser irradiation was associated with a decline in spatial learning and memory, as evidenced by decreased time spent in the target quadrant compared to the other quadrants (P < 0.001). Our findings indicate that the application of Rose Bengal alongside laser irradiation results in cellular injury, disruption of neural tissue, and impairment of learning and memory performance. [ABSTRACT FROM AUTHOR]
Copyright of Lasers in Medical Science is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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  Data: A step-by-step approach to minimally photothrombotic ischemic stroke in the hippocampal region that simulates human stroke.
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  Data: &lt;searchLink fieldCode=&quot;JN&quot; term=&quot;%22Lasers+in+Medical+Science%22&quot;&gt;Lasers in Medical Science&lt;/searchLink&gt;. 7/11/2025, Vol. 40 Issue 1, p1-11. 11p.
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  Data: The hippocampal structure, characterized by blood vessels with an average diameter of 0.5 mm, exhibits a significant vulnerability to ischemic conditions and is the critical region in the learning process and memory establishment. Male mice (n = 30) were divided into three groups (each group n = 10). 1) Rose Bengal group, 2) Laser group, 3) Rose Bengal + Laser group. Mice were placed in a stereotaxic device. An optical fiber was positioned above the ascending part of the hippocampal fissure and illuminated for 15 min using a previously injected intraperitoneal Rose Bengal dye. The Passive avoidance and MWM tests evaluated learning ability and memory capacity. TTC and Cresyl violet staining were conducted to assess the neural tissue morphology and histological alterations. Photochemical stroke resulted in hemorrhagic tissue and pale ischemic changes over three days. Microscopic analysis revealed neural tissue degeneration and disruption in the (Rose Bengal + Laser) group compared to the other experimental groups. The passive avoidance test demonstrated a statistically significant reduction in the time required to reach the dark compartment within the (Rose Bengal + Laser) group relative to the other groups (P &lt; 0.001). Furthermore, treatment with Rose Bengal and laser irradiation was associated with a decline in spatial learning and memory, as evidenced by decreased time spent in the target quadrant compared to the other quadrants (P &lt; 0.001). Our findings indicate that the application of Rose Bengal alongside laser irradiation results in cellular injury, disruption of neural tissue, and impairment of learning and memory performance. [ABSTRACT FROM AUTHOR]
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  Data: &lt;i&gt;Copyright of Lasers in Medical Science is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder&#39;s express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.&lt;/i&gt; (Copyright applies to all Abstracts.)
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        Value: 10.1007/s10103-025-04500-0
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        Text: English
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