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Combining QCM and SERS on a Nanophotonic Chip: A Dual-Functional Sensor for Biomolecular Interaction Analysis and Protein Fingerprinting.

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Title: Combining QCM and SERS on a Nanophotonic Chip: A Dual-Functional Sensor for Biomolecular Interaction Analysis and Protein Fingerprinting.
Authors: Bartolini, Cosimo1,2 (AUTHOR), Tozzetti, Martina1,2 (AUTHOR), Gellini, Cristina1 (AUTHOR), Ricci, Marilena1,2 (AUTHOR), Menichetti, Stefano1 (AUTHOR), Procacci, Piero1 (AUTHOR), Caminati, Gabriella1,2 (AUTHOR) gabriella.caminati@unifi.it
Source: Nanomaterials (2079-4991). Aug2025, Vol. 15 Issue 16, p1230. 24p.
Subjects: Biosensors, Quartz crystal microbalances, Molecular recognition, Nanophotonics, Intermolecular interactions, SERS spectroscopy, Peptide mass fingerprinting
Abstract: We present a dual biosensing strategy integrating Quartz Crystal Microbalance (QCM) and Surface-Enhanced Raman Spectroscopy (SERS) for the quantitative and molecular-specific detection of FKBP12. Silver nanodendritic arrays were electrodeposited onto QCM sensors, optimized for SERS enhancement using Rhodamine 6G, and functionalized with a custom-designed receptor to selectively capture FKBP12. QCM measurements revealed a two-step Langmuir adsorption behavior, enabling sensitive mass quantification with a low limit of detection. Concurrently, in situ SERS analysis on the same sensor provided vibrational fingerprints of FKBP12, resolved through comparative studies of the free protein, surface-bound receptor, and surface-bound receptor–protein complex. Ethanol-induced denaturation confirmed protein-specific peaks, while shifts in receptor vibrational modes—linked to FKBP12 binding—demonstrated dynamic molecular interactions. A ratiometric parameter, derived from key peak intensities, served as a robust, concentration-dependent signature of complex formation. This platform bridges quantitative (QCM) and structural (SERS) biosensing, offering real-time mass tracking and conformational insights. The nanodendritic substrate's dual functionality, combined with the receptor's selectivity, advances label-free protein detection for applications in drug diagnostics, with potential adaptability to other target analytes. [ABSTRACT FROM AUTHOR]
Copyright of Nanomaterials (2079-4991) is the property of MDPI and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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  Data: Combining QCM and SERS on a Nanophotonic Chip: A Dual-Functional Sensor for Biomolecular Interaction Analysis and Protein Fingerprinting.
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  Data: <searchLink fieldCode="AR" term="%22Bartolini%2C+Cosimo%22">Bartolini, Cosimo</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Tozzetti%2C+Martina%22">Tozzetti, Martina</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Gellini%2C+Cristina%22">Gellini, Cristina</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Ricci%2C+Marilena%22">Ricci, Marilena</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Menichetti%2C+Stefano%22">Menichetti, Stefano</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Procacci%2C+Piero%22">Procacci, Piero</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Caminati%2C+Gabriella%22">Caminati, Gabriella</searchLink><relatesTo>1,2</relatesTo> (AUTHOR)<i> gabriella.caminati@unifi.it</i>
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  Data: <searchLink fieldCode="JN" term="%22Nanomaterials+%282079-4991%29%22">Nanomaterials (2079-4991)</searchLink>. Aug2025, Vol. 15 Issue 16, p1230. 24p.
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  Data: <searchLink fieldCode="DE" term="%22Biosensors%22">Biosensors</searchLink><br /><searchLink fieldCode="DE" term="%22Quartz+crystal+microbalances%22">Quartz crystal microbalances</searchLink><br /><searchLink fieldCode="DE" term="%22Molecular+recognition%22">Molecular recognition</searchLink><br /><searchLink fieldCode="DE" term="%22Nanophotonics%22">Nanophotonics</searchLink><br /><searchLink fieldCode="DE" term="%22Intermolecular+interactions%22">Intermolecular interactions</searchLink><br /><searchLink fieldCode="DE" term="%22SERS+spectroscopy%22">SERS spectroscopy</searchLink><br /><searchLink fieldCode="DE" term="%22Peptide+mass+fingerprinting%22">Peptide mass fingerprinting</searchLink>
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  Data: We present a dual biosensing strategy integrating Quartz Crystal Microbalance (QCM) and Surface-Enhanced Raman Spectroscopy (SERS) for the quantitative and molecular-specific detection of FKBP12. Silver nanodendritic arrays were electrodeposited onto QCM sensors, optimized for SERS enhancement using Rhodamine 6G, and functionalized with a custom-designed receptor to selectively capture FKBP12. QCM measurements revealed a two-step Langmuir adsorption behavior, enabling sensitive mass quantification with a low limit of detection. Concurrently, in situ SERS analysis on the same sensor provided vibrational fingerprints of FKBP12, resolved through comparative studies of the free protein, surface-bound receptor, and surface-bound receptor–protein complex. Ethanol-induced denaturation confirmed protein-specific peaks, while shifts in receptor vibrational modes—linked to FKBP12 binding—demonstrated dynamic molecular interactions. A ratiometric parameter, derived from key peak intensities, served as a robust, concentration-dependent signature of complex formation. This platform bridges quantitative (QCM) and structural (SERS) biosensing, offering real-time mass tracking and conformational insights. The nanodendritic substrate's dual functionality, combined with the receptor's selectivity, advances label-free protein detection for applications in drug diagnostics, with potential adaptability to other target analytes. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Nanomaterials (2079-4991) is the property of MDPI and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Value: 10.3390/nano15161230
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        Text: English
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      – SubjectFull: Biosensors
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      – SubjectFull: Quartz crystal microbalances
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      – SubjectFull: Molecular recognition
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      – SubjectFull: Nanophotonics
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      – SubjectFull: Intermolecular interactions
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      – SubjectFull: SERS spectroscopy
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      – SubjectFull: Peptide mass fingerprinting
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      – TitleFull: Combining QCM and SERS on a Nanophotonic Chip: A Dual-Functional Sensor for Biomolecular Interaction Analysis and Protein Fingerprinting.
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              M: 08
              Text: Aug2025
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