A 3D printed device for vibration-assisted separation of different-stage mosquito larvae.

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Title: A 3D printed device for vibration-assisted separation of different-stage mosquito larvae.
Authors: Bursali, Fatma1 (AUTHOR) fgunerkan@gmail.com, Yetiskin, Erturan2 (AUTHOR), Ozcelik, Adem2 (AUTHOR)
Source: Instrumentation Science & Technology. 2025, Vol. 53 Issue 5, p499-514. 16p.
Subjects: Insect larvae, Larvae, Three-dimensional printing, Separation (Technology), Public health, Biological assay, Insect growth regulators
Abstract: Mosquitoes, as major vectors of dangerous diseases, demand effective control measures for public health. Larvicides, while offering a potential solution, can exhibit varying effects on different mosquito species and developmental stages during efficacy studies. Standardizing mosquito developmental stages and ensuring optimal, synchronized development rates of various larval stages are crucial for larvicidal experiments and maximizing pupal production. Manual separation of different stages of larvae is labor intensive, time consuming and error prone. Therefore, this study aimed to investigate the use of a stereolithography 3D-printed device for the separation of mosquito larvae. This device is designed to have three modules to collect different larval stages based on their radial size differences. Each module of the device was equipped with small vibration motors to enable vibration-assisted filtration to prevent clogging. The accuracy of the larvae separation device was studied by repeated experiments and collected samples were analyzed under an optical microscope. The 3D-printed device was demonstrated to be orders of magnitude faster and more accurate compared to manual separation process. A larvicidal bioassay was also designed to investigate larvicidal assay impact on the survival of collected L3 and L4 specimens. Overall, it was shown that the presented device carries an important potential for rapid and accurate separation of different size larvae for larvicidal studies. [ABSTRACT FROM AUTHOR]
Copyright of Instrumentation Science & Technology is the property of Taylor & Francis Ltd and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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DbLabel: Engineering Source
An: 187695948
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  Label: Title
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  Data: A 3D printed device for vibration-assisted separation of different-stage mosquito larvae.
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  Label: Authors
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  Data: <searchLink fieldCode="AR" term="%22Bursali%2C+Fatma%22">Bursali, Fatma</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> fgunerkan@gmail.com</i><br /><searchLink fieldCode="AR" term="%22Yetiskin%2C+Erturan%22">Yetiskin, Erturan</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Ozcelik%2C+Adem%22">Ozcelik, Adem</searchLink><relatesTo>2</relatesTo> (AUTHOR)
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  Data: <searchLink fieldCode="JN" term="%22Instrumentation+Science+%26+Technology%22">Instrumentation Science & Technology</searchLink>. 2025, Vol. 53 Issue 5, p499-514. 16p.
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  Data: <searchLink fieldCode="DE" term="%22Insect+larvae%22">Insect larvae</searchLink><br /><searchLink fieldCode="DE" term="%22Larvae%22">Larvae</searchLink><br /><searchLink fieldCode="DE" term="%22Three-dimensional+printing%22">Three-dimensional printing</searchLink><br /><searchLink fieldCode="DE" term="%22Separation+%28Technology%29%22">Separation (Technology)</searchLink><br /><searchLink fieldCode="DE" term="%22Public+health%22">Public health</searchLink><br /><searchLink fieldCode="DE" term="%22Biological+assay%22">Biological assay</searchLink><br /><searchLink fieldCode="DE" term="%22Insect+growth+regulators%22">Insect growth regulators</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Mosquitoes, as major vectors of dangerous diseases, demand effective control measures for public health. Larvicides, while offering a potential solution, can exhibit varying effects on different mosquito species and developmental stages during efficacy studies. Standardizing mosquito developmental stages and ensuring optimal, synchronized development rates of various larval stages are crucial for larvicidal experiments and maximizing pupal production. Manual separation of different stages of larvae is labor intensive, time consuming and error prone. Therefore, this study aimed to investigate the use of a stereolithography 3D-printed device for the separation of mosquito larvae. This device is designed to have three modules to collect different larval stages based on their radial size differences. Each module of the device was equipped with small vibration motors to enable vibration-assisted filtration to prevent clogging. The accuracy of the larvae separation device was studied by repeated experiments and collected samples were analyzed under an optical microscope. The 3D-printed device was demonstrated to be orders of magnitude faster and more accurate compared to manual separation process. A larvicidal bioassay was also designed to investigate larvicidal assay impact on the survival of collected L3 and L4 specimens. Overall, it was shown that the presented device carries an important potential for rapid and accurate separation of different size larvae for larvicidal studies. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Instrumentation Science & Technology is the property of Taylor & Francis Ltd and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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RecordInfo BibRecord:
  BibEntity:
    Identifiers:
      – Type: doi
        Value: 10.1080/10739149.2024.2418840
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      – Code: eng
        Text: English
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        PageCount: 16
        StartPage: 499
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      – SubjectFull: Insect larvae
        Type: general
      – SubjectFull: Larvae
        Type: general
      – SubjectFull: Three-dimensional printing
        Type: general
      – SubjectFull: Separation (Technology)
        Type: general
      – SubjectFull: Public health
        Type: general
      – SubjectFull: Biological assay
        Type: general
      – SubjectFull: Insect growth regulators
        Type: general
    Titles:
      – TitleFull: A 3D printed device for vibration-assisted separation of different-stage mosquito larvae.
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            NameFull: Bursali, Fatma
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            NameFull: Yetiskin, Erturan
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            NameFull: Ozcelik, Adem
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            – D: 01
              M: 09
              Text: 2025
              Type: published
              Y: 2025
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