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From shake flasks to a bench‐scale bioreactor: scaling up exopolysaccharide production in fed‐batch cultivation of Ensifer meliloti SEMIA 135.

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Title: From shake flasks to a bench‐scale bioreactor: scaling up exopolysaccharide production in fed‐batch cultivation of Ensifer meliloti SEMIA 135.
Authors: Ferreira Filho, Rui dos Santos1 (AUTHOR) ruiferreirafilho@outlook.com, Burkert, Janaína Fernandes de Medeiros1 (AUTHOR), Burkert, Carlos André Veiga1 (AUTHOR)
Source: Journal of Chemical Technology & Biotechnology. Sep2025, Vol. 100 Issue 9, p1818-1830. 13p.
Subjects: Molasses, Bioreactors, Rhizobium meliloti, Biopolymers, Industrial costs, Industrial applications, Biochemical engineering
Abstract: BACKGROUND: This study investigates the production scale‐up of exopolysaccharide (EPS) by Ensifer meliloti SEMIA 135 from shake flasks to a 5 L bench‐scale fed‐batch bioreactor. Soybean molasses was the cost‐effective nutrient source. The primary aim was to improve conditions for maximizing EPS yield and minimizing production costs. RESULTS: Various molasses concentrations (30 to 50 g L−1), with and without yeast extract and mineral salts, were tested in shake flasks. Agitation and operational modes (batch and fed‐batch) were then evaluated in the bioreactor. Results showed that yeast extract is unnecessary (a cost‐reduction factor) since soybean molasses supplemented with mineral salts achieved similar EPS production. The highest molasses concentrations inhibited the EPS synthesis. Best‐performing bioreactor conditions (200 rpm, 1.25 vvm) in the fed‐batch mode yielded 14.4 g L−1 EPS. It meant increase of 67% by comparison with the batch mode. EPS exhibited high molecular weight (9.266 × 107 Da), polyanionic character (zeta potential −36.03 mV at pH 7) and functional groups which are typical of rhizobial EPS. Its rheological behavior followed the Herschel‐Bulkley model, i.e., viscosity decreased at high pH, and it showed remarkable thermal stability (melting point was 203 °C). CONCLUSION: This study highlights the potential of soybean molasses in cost‐effective EPS production. Fed‐batch conditions improved EPS yields significantly. EPS properties underline its potential for industrial applications and, thus, generation of knowledge about scalable biopolymer production. © 2025 The Author(s). Journal of Chemical Technology and Biotechnology published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry (SCI). [ABSTRACT FROM AUTHOR]
Copyright of Journal of Chemical Technology & Biotechnology is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
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DbLabel: Engineering Source
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  Data: From shake flasks to a bench‐scale bioreactor: scaling up exopolysaccharide production in fed‐batch cultivation of Ensifer meliloti SEMIA 135.
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  Data: <searchLink fieldCode="JN" term="%22Journal+of+Chemical+Technology+%26+Biotechnology%22">Journal of Chemical Technology & Biotechnology</searchLink>. Sep2025, Vol. 100 Issue 9, p1818-1830. 13p.
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  Data: <searchLink fieldCode="DE" term="%22Molasses%22">Molasses</searchLink><br /><searchLink fieldCode="DE" term="%22Bioreactors%22">Bioreactors</searchLink><br /><searchLink fieldCode="DE" term="%22Rhizobium+meliloti%22">Rhizobium meliloti</searchLink><br /><searchLink fieldCode="DE" term="%22Biopolymers%22">Biopolymers</searchLink><br /><searchLink fieldCode="DE" term="%22Industrial+costs%22">Industrial costs</searchLink><br /><searchLink fieldCode="DE" term="%22Industrial+applications%22">Industrial applications</searchLink><br /><searchLink fieldCode="DE" term="%22Biochemical+engineering%22">Biochemical engineering</searchLink>
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  Data: BACKGROUND: This study investigates the production scale‐up of exopolysaccharide (EPS) by Ensifer meliloti SEMIA 135 from shake flasks to a 5 L bench‐scale fed‐batch bioreactor. Soybean molasses was the cost‐effective nutrient source. The primary aim was to improve conditions for maximizing EPS yield and minimizing production costs. RESULTS: Various molasses concentrations (30 to 50 g L−1), with and without yeast extract and mineral salts, were tested in shake flasks. Agitation and operational modes (batch and fed‐batch) were then evaluated in the bioreactor. Results showed that yeast extract is unnecessary (a cost‐reduction factor) since soybean molasses supplemented with mineral salts achieved similar EPS production. The highest molasses concentrations inhibited the EPS synthesis. Best‐performing bioreactor conditions (200 rpm, 1.25 vvm) in the fed‐batch mode yielded 14.4 g L−1 EPS. It meant increase of 67% by comparison with the batch mode. EPS exhibited high molecular weight (9.266 × 107 Da), polyanionic character (zeta potential −36.03 mV at pH 7) and functional groups which are typical of rhizobial EPS. Its rheological behavior followed the Herschel‐Bulkley model, i.e., viscosity decreased at high pH, and it showed remarkable thermal stability (melting point was 203 °C). CONCLUSION: This study highlights the potential of soybean molasses in cost‐effective EPS production. Fed‐batch conditions improved EPS yields significantly. EPS properties underline its potential for industrial applications and, thus, generation of knowledge about scalable biopolymer production. © 2025 The Author(s). Journal of Chemical Technology and Biotechnology published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry (SCI). [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Journal of Chemical Technology & Biotechnology is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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RecordInfo BibRecord:
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      – Type: doi
        Value: 10.1002/jctb.7916
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      – Code: eng
        Text: English
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        PageCount: 13
        StartPage: 1818
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      – SubjectFull: Molasses
        Type: general
      – SubjectFull: Bioreactors
        Type: general
      – SubjectFull: Rhizobium meliloti
        Type: general
      – SubjectFull: Biopolymers
        Type: general
      – SubjectFull: Industrial costs
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      – SubjectFull: Industrial applications
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      – SubjectFull: Biochemical engineering
        Type: general
    Titles:
      – TitleFull: From shake flasks to a bench‐scale bioreactor: scaling up exopolysaccharide production in fed‐batch cultivation of Ensifer meliloti SEMIA 135.
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      – PersonEntity:
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            NameFull: Ferreira Filho, Rui dos Santos
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            NameFull: Burkert, Janaína Fernandes de Medeiros
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            NameFull: Burkert, Carlos André Veiga
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            – D: 01
              M: 09
              Text: Sep2025
              Type: published
              Y: 2025
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