View in EDS

Co-substrate utilisation in "Candidatus Accumulibacter" enhances metabolic fitness in dynamic environments.

Saved in:
Bibliographic Details
Title: Co-substrate utilisation in "Candidatus Accumulibacter" enhances metabolic fitness in dynamic environments.
Authors: Páez-Watson, Timothy1 (AUTHOR), Jansens, Casper1 (AUTHOR), van Loosdrecht, Mark C.M.1 (AUTHOR), Roy, Samarpita1 (AUTHOR) samarpita.roy@tudelft.nl
Source: Water Research. Dec2025:Part A, Vol. 287, pN.PAG-N.PAG. 1p.
Subjects: Wastewater treatment, Microbial communities, Microbial ecology, Biomass production, Metabolism
Abstract: • Co-substrate utilization leads to synergistic metabolic interactions in " Ca. Accumulibacter". • Aspartate and acetate co-use lowers ATP loss and glycogen dependence, increasing growth efficiency. • Observed synergy highlights that co-substrates reshape metabolic strategies. • Reciprocal synergy of co-substrates boosts biomass yield beyond individual substrate yields. • In WWTPs, synergistic effects of co-substrate use can be exploited to improve process performance. Optimizing resource use is essential for the survival and fitness of species in microbial communities ubiquitous in natural and engineered ecosystems. These ecosystems are often characterized by the simultaneous presence of multiple substrates such as volatile fatty acids, amino acids and sugars. Yet, the evaluation of metabolic potential for these microbial community members is predominantly based on single substrate utilisation. Metabolic and ecological implications of the interactions of multiple substrates, particularly in environments with changes in redox conditions and substrate availability, remain poorly understood. In this study, we investigate the metabolic interactions resulting from co-substrate utilization in polyphosphate-accumulating organisms within wastewater treatment systems. We combined experimental analysis of highly enriched " Ca. Accumulibacter" mixed cultures with genome-resolved metagenomics and conditional flux balance analysis (cFBA) to quantify the physiological relevance of co-substrate uptake. We observe that anaerobic co-substrate utilisation of acetate and aspartate result in metabolic interactions leading to optimized redox balance, reduced ATP losses and increased biomass yields by up to 8% compared to individual substrate use. Metabolic modelling revealed that these benefits emerge from the network topology, where the interaction of different metabolic routes gives rise to synergistic effects. Extending our analysis to additional substrate pairs, we classify metabolic interactions into three general types: (i) neutral, (ii) one-way synergistic and (iii) reciprocal synergistic. Our findings highlight the importance of metabolic interactions and cellular resource allocation strategies in dynamic microbial ecosystems. This study provides a broader ecological framework for understanding competitive metabolic strategies in environmental organisms. Co-substrate utilization can have direct implications for improving the yield or productivity of bioprocesses. [ABSTRACT FROM AUTHOR]
Copyright of Water Research is the property of Pergamon Press - An Imprint of Elsevier Science and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
FullText Text:
  Availability: 0
Header DbId: egs
DbLabel: Engineering Source
An: 188679839
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 0
IllustrationInfo
Items – Name: Title
  Label: Title
  Group: Ti
  Data: Co-substrate utilisation in "Candidatus Accumulibacter" enhances metabolic fitness in dynamic environments.
– Name: Author
  Label: Authors
  Group: Au
  Data: <searchLink fieldCode="AR" term="%22Páez-Watson%2C+Timothy%22">Páez-Watson, Timothy</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Jansens%2C+Casper%22">Jansens, Casper</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22van+Loosdrecht%2C+Mark+C%2EM%2E%22">van Loosdrecht, Mark C.M.</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Roy%2C+Samarpita%22">Roy, Samarpita</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> samarpita.roy@tudelft.nl</i>
– Name: TitleSource
  Label: Source
  Group: Src
  Data: <searchLink fieldCode="JN" term="%22Water+Research%22">Water Research</searchLink>. Dec2025:Part A, Vol. 287, pN.PAG-N.PAG. 1p.
– Name: Subject
  Label: Subjects
  Group: Su
  Data: <searchLink fieldCode="DE" term="%22Wastewater+treatment%22">Wastewater treatment</searchLink><br /><searchLink fieldCode="DE" term="%22Microbial+communities%22">Microbial communities</searchLink><br /><searchLink fieldCode="DE" term="%22Microbial+ecology%22">Microbial ecology</searchLink><br /><searchLink fieldCode="DE" term="%22Biomass+production%22">Biomass production</searchLink><br /><searchLink fieldCode="DE" term="%22Metabolism%22">Metabolism</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: • Co-substrate utilization leads to synergistic metabolic interactions in " Ca. Accumulibacter". • Aspartate and acetate co-use lowers ATP loss and glycogen dependence, increasing growth efficiency. • Observed synergy highlights that co-substrates reshape metabolic strategies. • Reciprocal synergy of co-substrates boosts biomass yield beyond individual substrate yields. • In WWTPs, synergistic effects of co-substrate use can be exploited to improve process performance. Optimizing resource use is essential for the survival and fitness of species in microbial communities ubiquitous in natural and engineered ecosystems. These ecosystems are often characterized by the simultaneous presence of multiple substrates such as volatile fatty acids, amino acids and sugars. Yet, the evaluation of metabolic potential for these microbial community members is predominantly based on single substrate utilisation. Metabolic and ecological implications of the interactions of multiple substrates, particularly in environments with changes in redox conditions and substrate availability, remain poorly understood. In this study, we investigate the metabolic interactions resulting from co-substrate utilization in polyphosphate-accumulating organisms within wastewater treatment systems. We combined experimental analysis of highly enriched " Ca. Accumulibacter" mixed cultures with genome-resolved metagenomics and conditional flux balance analysis (cFBA) to quantify the physiological relevance of co-substrate uptake. We observe that anaerobic co-substrate utilisation of acetate and aspartate result in metabolic interactions leading to optimized redox balance, reduced ATP losses and increased biomass yields by up to 8% compared to individual substrate use. Metabolic modelling revealed that these benefits emerge from the network topology, where the interaction of different metabolic routes gives rise to synergistic effects. Extending our analysis to additional substrate pairs, we classify metabolic interactions into three general types: (i) neutral, (ii) one-way synergistic and (iii) reciprocal synergistic. Our findings highlight the importance of metabolic interactions and cellular resource allocation strategies in dynamic microbial ecosystems. This study provides a broader ecological framework for understanding competitive metabolic strategies in environmental organisms. Co-substrate utilization can have direct implications for improving the yield or productivity of bioprocesses. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Water Research is the property of Pergamon Press - An Imprint of Elsevier Science and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
PLink https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=egs&AN=188679839
RecordInfo BibRecord:
  BibEntity:
    Identifiers:
      – Type: doi
        Value: 10.1016/j.watres.2025.124401
    Languages:
      – Code: eng
        Text: English
    PhysicalDescription:
      Pagination:
        PageCount: 1
        StartPage: N.PAG
    Subjects:
      – SubjectFull: Wastewater treatment
        Type: general
      – SubjectFull: Microbial communities
        Type: general
      – SubjectFull: Microbial ecology
        Type: general
      – SubjectFull: Biomass production
        Type: general
      – SubjectFull: Metabolism
        Type: general
    Titles:
      – TitleFull: Co-substrate utilisation in "Candidatus Accumulibacter" enhances metabolic fitness in dynamic environments.
        Type: main
  BibRelationships:
    HasContributorRelationships:
      – PersonEntity:
          Name:
            NameFull: Páez-Watson, Timothy
      – PersonEntity:
          Name:
            NameFull: Jansens, Casper
      – PersonEntity:
          Name:
            NameFull: van Loosdrecht, Mark C.M.
      – PersonEntity:
          Name:
            NameFull: Roy, Samarpita
    IsPartOfRelationships:
      – BibEntity:
          Dates:
            – D: 01
              M: 12
              Text: Dec2025:Part A
              Type: published
              Y: 2025
          Identifiers:
            – Type: issn-print
              Value: 00431354
          Numbering:
            – Type: volume
              Value: 287
          Titles:
            – TitleFull: Water Research
              Type: main
ResultId 1