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Crucial roles of intracellular cyclic di-GMP in impacting the genes important for extracellular electron transfer by Geobacter metallireducens.

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Title: Crucial roles of intracellular cyclic di-GMP in impacting the genes important for extracellular electron transfer by Geobacter metallireducens.
Authors: Yongguang Jiang1 jiangyg@cug.edu.cn, Lin Sun1, Lingyu Hou1, Yidan Hu1, Zhou Jiang1, Yiran Dong1,2,3,4, Hao Song5, Liang Shi1,2,3,4 liang.shi@cug.edu.cn
Source: Applied & Environmental Microbiology. Jul2025, Vol. 91 Issue 7, p1-14. 14p.
Subjects: Electron transport, Cytochrome c, Genetic regulation, Synthetic biology, Geobacter sulfurreducens, Applied sciences, Cyclic guanylic acid, Biofilms
Abstract: To investigate the roles of intracellular c-di-GMP in bacterial extracellular electron transfer (EET), three Geobacter metallireducens strains with high (Gme-H), intermediate (Gme-C), and low (Gme-L) intracellular levels of c-di-GMP were constructed via the synthetic biology approach. Compared to Gme-C, Gme-H showed similar Fe(III) reduction rates, formed thicker biofilms on conductive and nonconductive surfaces, and produced more electricity, but showed delayed ability for electricity production. Gme-L formed thinner biofilms on nonconductive surfaces and reduced Fe(III)-citrate faster, but showed slower reduction of ferrihydrite in comparison to Gme-C. Although it produced electricity much faster, Gme-L produced less electricity and formed slightly less amounts of biofilms on anodes, as compared to Gme-C. The mRNA levels of multiple genes encoding c-type cytochromes (c-Cyts) and extracellular pilin protein PilA-N were differentially regulated in Gme-L or Gme-H in comparison to that in Gme-C. Expressions of the genes for PilA-N and extracellular c-Cyt Gmet2896 were increased by high c-di-GMP. Low c-di-GMP increased the gene expressions of the porin-cytochromes in the outer membrane. Further investigation also identified new c-di-GMP-regulated genes directly involved in the EET of G. metallireducens, such as those for the c-Cyts of extracellular Gmet0601, the periplasmic Gmet1703 and Gmet1809 on the cytoplasmic membrane, as deletions of these genes impaired bacterial reductions of extracellular ferrihydrite and anode. Thus, intracellular c-di-GMP impacted multiple genes of G. metallireducens whose protein products might transfer electrons from the cytoplasmic membrane, through the periplasm, across the outer membrane to and in the extracellular environment. [ABSTRACT FROM AUTHOR]
Copyright of Applied & Environmental Microbiology is the property of American Society for Microbiology and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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DbLabel: Engineering Source
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  Data: Crucial roles of intracellular cyclic di-GMP in impacting the genes important for extracellular electron transfer by Geobacter metallireducens.
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  Data: <searchLink fieldCode="AR" term="%22Yongguang+Jiang%22">Yongguang Jiang</searchLink><relatesTo>1</relatesTo><i> jiangyg@cug.edu.cn</i><br /><searchLink fieldCode="AR" term="%22Lin+Sun%22">Lin Sun</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Lingyu+Hou%22">Lingyu Hou</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Yidan+Hu%22">Yidan Hu</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Zhou+Jiang%22">Zhou Jiang</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Yiran+Dong%22">Yiran Dong</searchLink><relatesTo>1,2,3,4</relatesTo><br /><searchLink fieldCode="AR" term="%22Hao+Song%22">Hao Song</searchLink><relatesTo>5</relatesTo><br /><searchLink fieldCode="AR" term="%22Liang+Shi%22">Liang Shi</searchLink><relatesTo>1,2,3,4</relatesTo><i> liang.shi@cug.edu.cn</i>
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  Data: <searchLink fieldCode="JN" term="%22Applied+%26+Environmental+Microbiology%22">Applied & Environmental Microbiology</searchLink>. Jul2025, Vol. 91 Issue 7, p1-14. 14p.
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  Data: <searchLink fieldCode="DE" term="%22Electron+transport%22">Electron transport</searchLink><br /><searchLink fieldCode="DE" term="%22Cytochrome+c%22">Cytochrome c</searchLink><br /><searchLink fieldCode="DE" term="%22Genetic+regulation%22">Genetic regulation</searchLink><br /><searchLink fieldCode="DE" term="%22Synthetic+biology%22">Synthetic biology</searchLink><br /><searchLink fieldCode="DE" term="%22Geobacter+sulfurreducens%22">Geobacter sulfurreducens</searchLink><br /><searchLink fieldCode="DE" term="%22Applied+sciences%22">Applied sciences</searchLink><br /><searchLink fieldCode="DE" term="%22Cyclic+guanylic+acid%22">Cyclic guanylic acid</searchLink><br /><searchLink fieldCode="DE" term="%22Biofilms%22">Biofilms</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: To investigate the roles of intracellular c-di-GMP in bacterial extracellular electron transfer (EET), three Geobacter metallireducens strains with high (Gme-H), intermediate (Gme-C), and low (Gme-L) intracellular levels of c-di-GMP were constructed via the synthetic biology approach. Compared to Gme-C, Gme-H showed similar Fe(III) reduction rates, formed thicker biofilms on conductive and nonconductive surfaces, and produced more electricity, but showed delayed ability for electricity production. Gme-L formed thinner biofilms on nonconductive surfaces and reduced Fe(III)-citrate faster, but showed slower reduction of ferrihydrite in comparison to Gme-C. Although it produced electricity much faster, Gme-L produced less electricity and formed slightly less amounts of biofilms on anodes, as compared to Gme-C. The mRNA levels of multiple genes encoding c-type cytochromes (c-Cyts) and extracellular pilin protein PilA-N were differentially regulated in Gme-L or Gme-H in comparison to that in Gme-C. Expressions of the genes for PilA-N and extracellular c-Cyt Gmet2896 were increased by high c-di-GMP. Low c-di-GMP increased the gene expressions of the porin-cytochromes in the outer membrane. Further investigation also identified new c-di-GMP-regulated genes directly involved in the EET of G. metallireducens, such as those for the c-Cyts of extracellular Gmet0601, the periplasmic Gmet1703 and Gmet1809 on the cytoplasmic membrane, as deletions of these genes impaired bacterial reductions of extracellular ferrihydrite and anode. Thus, intracellular c-di-GMP impacted multiple genes of G. metallireducens whose protein products might transfer electrons from the cytoplasmic membrane, through the periplasm, across the outer membrane to and in the extracellular environment. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Applied & Environmental Microbiology is the property of American Society for Microbiology and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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RecordInfo BibRecord:
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      – Type: doi
        Value: 10.1128/aem.00727-25
    Languages:
      – Code: eng
        Text: English
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      Pagination:
        PageCount: 14
        StartPage: 1
    Subjects:
      – SubjectFull: Electron transport
        Type: general
      – SubjectFull: Cytochrome c
        Type: general
      – SubjectFull: Genetic regulation
        Type: general
      – SubjectFull: Synthetic biology
        Type: general
      – SubjectFull: Geobacter sulfurreducens
        Type: general
      – SubjectFull: Applied sciences
        Type: general
      – SubjectFull: Cyclic guanylic acid
        Type: general
      – SubjectFull: Biofilms
        Type: general
    Titles:
      – TitleFull: Crucial roles of intracellular cyclic di-GMP in impacting the genes important for extracellular electron transfer by Geobacter metallireducens.
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            NameFull: Yongguang Jiang
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            NameFull: Lin Sun
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            NameFull: Lingyu Hou
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              M: 07
              Text: Jul2025
              Type: published
              Y: 2025
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