Crucial roles of intracellular cyclic di-GMP in impacting the genes important for extracellular electron transfer by Geobacter metallireducens.
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| Title: | Crucial roles of intracellular cyclic di-GMP in impacting the genes important for extracellular electron transfer by Geobacter metallireducens. |
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| Authors: | Yongguang Jiang1 jiangyg@cug.edu.cn, Lin Sun1, Lingyu Hou1, Yidan Hu1, Zhou Jiang1, Yiran Dong1,2,3,4, Hao Song5, Liang Shi1,2,3,4 liang.shi@cug.edu.cn |
| Source: | Applied & Environmental Microbiology. Jul2025, Vol. 91 Issue 7, p1-14. 14p. |
| Subjects: | Electron transport, Cytochrome c, Genetic regulation, Synthetic biology, Geobacter sulfurreducens, Applied sciences, Cyclic guanylic acid, Biofilms |
| Abstract: | To investigate the roles of intracellular c-di-GMP in bacterial extracellular electron transfer (EET), three Geobacter metallireducens strains with high (Gme-H), intermediate (Gme-C), and low (Gme-L) intracellular levels of c-di-GMP were constructed via the synthetic biology approach. Compared to Gme-C, Gme-H showed similar Fe(III) reduction rates, formed thicker biofilms on conductive and nonconductive surfaces, and produced more electricity, but showed delayed ability for electricity production. Gme-L formed thinner biofilms on nonconductive surfaces and reduced Fe(III)-citrate faster, but showed slower reduction of ferrihydrite in comparison to Gme-C. Although it produced electricity much faster, Gme-L produced less electricity and formed slightly less amounts of biofilms on anodes, as compared to Gme-C. The mRNA levels of multiple genes encoding c-type cytochromes (c-Cyts) and extracellular pilin protein PilA-N were differentially regulated in Gme-L or Gme-H in comparison to that in Gme-C. Expressions of the genes for PilA-N and extracellular c-Cyt Gmet2896 were increased by high c-di-GMP. Low c-di-GMP increased the gene expressions of the porin-cytochromes in the outer membrane. Further investigation also identified new c-di-GMP-regulated genes directly involved in the EET of G. metallireducens, such as those for the c-Cyts of extracellular Gmet0601, the periplasmic Gmet1703 and Gmet1809 on the cytoplasmic membrane, as deletions of these genes impaired bacterial reductions of extracellular ferrihydrite and anode. Thus, intracellular c-di-GMP impacted multiple genes of G. metallireducens whose protein products might transfer electrons from the cytoplasmic membrane, through the periplasm, across the outer membrane to and in the extracellular environment. [ABSTRACT FROM AUTHOR] |
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| Database: | Engineering Source |
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