Antioxidant and Neuroprotective Activities of Gastrodia elata Blume Aqueous Extract in Caenorhabditis elegans.
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| Title: | Antioxidant and Neuroprotective Activities of Gastrodia elata Blume Aqueous Extract in Caenorhabditis elegans. |
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| Authors: | Ma, Zhonglian1,2,3 (AUTHOR), Ma, Liang1 (AUTHOR) zhyhml@swu.edu.cn, Zhang, Yuhao1 (AUTHOR), Maltar Strmecki, Nadica (AUTHOR) nstrm@irb.hr |
| Source: | Journal of Food Biochemistry. 11/9/2025, Vol. 2025, p1-17. 17p. |
| Subjects: | Neuroprotective agents, Antioxidants, Caenorhabditis elegans, Free radicals, GABA, Gene expression, Serotonin |
| Abstract: | Gastrodia elata Blume (GE) is recognized as a plant with both nutritional and medicinal properties, reported to exhibit neuroprotective and antioxidant effects, which contribute to its widespread use in various fields. However, the mechanisms underlying these effects remain insufficiently understood. In this study, we utilized ultrasound‐assisted extraction to isolate water‐soluble substances from GE and conducted in vitro antioxidant assays to evaluate the capacity of Gastrodia elata water extract (GWE) to scavenge DPPH and ABTS free radicals. Further investigation into the antioxidant and neuroprotective potential of GWE was conducted through in vivo studies using Caenorhabditis elegans (C. elegans). It was observed that GWE at concentrations of 50, 100, and 200 μg/mL enhanced locomotion and antistress capabilities while significantly reducing levels of H2O2, O2−, lipofuscin, and reactive oxygen species (ROS). Concurrently, glutathione levels were significantly elevated. GWE supplementation stimulated the activity of serotonergic, dopaminergic, and GABAergic neurons, thereby improving locomotor abilities, including body bends, head thrashing, foraging behavior, and chemotaxis. Notably, significant increases in the relative concentrations of gamma‐aminobutyric acid (GABA) and serotonin were also observed. Further research demonstrated that GWE treatment elevated the mRNA levels of genes associated with GABA and serotonin in the treated nematodes, specifically the unc-25, unc-46, unc-49, unc-47, tph-1, mod-1, mod-5, cat-4, cat-1, and exp-1 genes. Additionally, GWE treatment significantly enhanced the expression of the daf-16, sod-3, skn-1, hsf-1, and hsp-16.2 genes. GWE also promoted the translocation of DAF‐16 into the nucleus, upregulated the expression of SOD‐3::GFP, and downregulated the expression of gst-4 and GST‐4::GFP. Spearman's correlation analysis revealed a significant positive association between the daf-16, hsp-16.2, cat-1, unc-46, unc-47, and mod-1 genes and the levels of serotonin, GABA, and locomotor behaviors. This study enhances our understanding of the mechanisms through which GWE exhibits neuroprotective and antioxidant properties, thereby elucidating the health benefits of GE for living organisms. [ABSTRACT FROM AUTHOR] |
| Copyright of Journal of Food Biochemistry is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
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| Header | DbId: egs DbLabel: Engineering Source An: 189214266 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Antioxidant and Neuroprotective Activities of Gastrodia elata Blume Aqueous Extract in Caenorhabditis elegans. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Ma%2C+Zhonglian%22">Ma, Zhonglian</searchLink><relatesTo>1,2,3</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Ma%2C+Liang%22">Ma, Liang</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> zhyhml@swu.edu.cn</i><br /><searchLink fieldCode="AR" term="%22Zhang%2C+Yuhao%22">Zhang, Yuhao</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Maltar+Strmecki%2C+Nadica%22">Maltar Strmecki, Nadica</searchLink> (AUTHOR)<i> nstrm@irb.hr</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Journal+of+Food+Biochemistry%22">Journal of Food Biochemistry</searchLink>. 11/9/2025, Vol. 2025, p1-17. 17p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Neuroprotective+agents%22">Neuroprotective agents</searchLink><br /><searchLink fieldCode="DE" term="%22Antioxidants%22">Antioxidants</searchLink><br /><searchLink fieldCode="DE" term="%22Caenorhabditis+elegans%22">Caenorhabditis elegans</searchLink><br /><searchLink fieldCode="DE" term="%22Free+radicals%22">Free radicals</searchLink><br /><searchLink fieldCode="DE" term="%22GABA%22">GABA</searchLink><br /><searchLink fieldCode="DE" term="%22Gene+expression%22">Gene expression</searchLink><br /><searchLink fieldCode="DE" term="%22Serotonin%22">Serotonin</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Gastrodia elata Blume (GE) is recognized as a plant with both nutritional and medicinal properties, reported to exhibit neuroprotective and antioxidant effects, which contribute to its widespread use in various fields. However, the mechanisms underlying these effects remain insufficiently understood. In this study, we utilized ultrasound‐assisted extraction to isolate water‐soluble substances from GE and conducted in vitro antioxidant assays to evaluate the capacity of Gastrodia elata water extract (GWE) to scavenge DPPH and ABTS free radicals. Further investigation into the antioxidant and neuroprotective potential of GWE was conducted through in vivo studies using Caenorhabditis elegans (C. elegans). It was observed that GWE at concentrations of 50, 100, and 200 μg/mL enhanced locomotion and antistress capabilities while significantly reducing levels of H2O2, O2−, lipofuscin, and reactive oxygen species (ROS). Concurrently, glutathione levels were significantly elevated. GWE supplementation stimulated the activity of serotonergic, dopaminergic, and GABAergic neurons, thereby improving locomotor abilities, including body bends, head thrashing, foraging behavior, and chemotaxis. Notably, significant increases in the relative concentrations of gamma‐aminobutyric acid (GABA) and serotonin were also observed. Further research demonstrated that GWE treatment elevated the mRNA levels of genes associated with GABA and serotonin in the treated nematodes, specifically the unc-25, unc-46, unc-49, unc-47, tph-1, mod-1, mod-5, cat-4, cat-1, and exp-1 genes. Additionally, GWE treatment significantly enhanced the expression of the daf-16, sod-3, skn-1, hsf-1, and hsp-16.2 genes. GWE also promoted the translocation of DAF‐16 into the nucleus, upregulated the expression of SOD‐3::GFP, and downregulated the expression of gst-4 and GST‐4::GFP. Spearman's correlation analysis revealed a significant positive association between the daf-16, hsp-16.2, cat-1, unc-46, unc-47, and mod-1 genes and the levels of serotonin, GABA, and locomotor behaviors. This study enhances our understanding of the mechanisms through which GWE exhibits neuroprotective and antioxidant properties, thereby elucidating the health benefits of GE for living organisms. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Journal of Food Biochemistry is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1155/jfbc/3191604 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 17 StartPage: 1 Subjects: – SubjectFull: Neuroprotective agents Type: general – SubjectFull: Antioxidants Type: general – SubjectFull: Caenorhabditis elegans Type: general – SubjectFull: Free radicals Type: general – SubjectFull: GABA Type: general – SubjectFull: Gene expression Type: general – SubjectFull: Serotonin Type: general Titles: – TitleFull: Antioxidant and Neuroprotective Activities of Gastrodia elata Blume Aqueous Extract in Caenorhabditis elegans. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Ma, Zhonglian – PersonEntity: Name: NameFull: Ma, Liang – PersonEntity: Name: NameFull: Zhang, Yuhao – PersonEntity: Name: NameFull: Maltar Strmecki, Nadica IsPartOfRelationships: – BibEntity: Dates: – D: 09 M: 11 Text: 11/9/2025 Type: published Y: 2025 Identifiers: – Type: issn-print Value: 01458884 Numbering: – Type: volume Value: 2025 Titles: – TitleFull: Journal of Food Biochemistry Type: main |
| ResultId | 1 |
