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Amine-reactive Polymer Platform for Engineering Surface Modification of Next-generation Sequencing Chips.

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Title: Amine-reactive Polymer Platform for Engineering Surface Modification of Next-generation Sequencing Chips.
Authors: Tian, Wei1 (AUTHOR), Wang, Xin-Yuan1 (AUTHOR), Feng, Die-Wen2 (AUTHOR), Li, Xiang-Qian1 (AUTHOR), Jin, Yue-Kang3 (AUTHOR) jinyk@mail.ustc.edu.cn, Li, Hui1 (AUTHOR) lihui@dhu.edu.cn, Liu, Hao1 (AUTHOR) liuh@dhu.edu.cn
Source: Chinese Journal of Polymer Science (Springer Science & Business Media B.V.). Nov2025, Vol. 43 Issue 11, p2030-2041. 12p.
Subjects: Click chemistry, Reactive polymers, Genetic techniques, Nucleotide sequencing, Individualized medicine, Biocompatibility, Surface preparation, Immobilized nucleic acids
Abstract: In this study, an amine-reactive poly(pentafluorophenyl acrylate) (PPFPA) platform was developed for advanced surface engineering of next-generation sequencing (NGS) chips. Through post-polymerization modification, PPFPA was functionalized with dual moieties: azide groups for covalent immobilization of DBCO-modified DNA primers via click chemistry and tunable hydrophilic side chains to optimize biocompatibility and surface properties. Systematic screening revealed that hydrophobic azide carriers combined with neutral hydroxyl groups maximized the DNA immobilization efficacy, approaching the performance of commercial polyacrylamide-based polymers. The negatively charged carboxyl groups severely impede DNA primer attachment. Higher molecular weight derivatives further enhance the efficacy of DNA immobilization. In NGS validation, optimized surface modification polymers achieved robust surface density of clustered DNA and high sequencing accuracy, surpassing quality benchmarks and comparable to those of conventional analogs. This platform demonstrates significant potential for tailoring high-sensitivity surfaces for genomic applications, advancing clinical diagnostics, and personalized medicine. [ABSTRACT FROM AUTHOR]
Copyright of Chinese Journal of Polymer Science (Springer Science & Business Media B.V.) is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
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DbLabel: Engineering Source
An: 189391165
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 0
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  Data: Amine-reactive Polymer Platform for Engineering Surface Modification of Next-generation Sequencing Chips.
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  Data: <searchLink fieldCode="AR" term="%22Tian%2C+Wei%22">Tian, Wei</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Wang%2C+Xin-Yuan%22">Wang, Xin-Yuan</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Feng%2C+Die-Wen%22">Feng, Die-Wen</searchLink><relatesTo>2</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Li%2C+Xiang-Qian%22">Li, Xiang-Qian</searchLink><relatesTo>1</relatesTo> (AUTHOR)<br /><searchLink fieldCode="AR" term="%22Jin%2C+Yue-Kang%22">Jin, Yue-Kang</searchLink><relatesTo>3</relatesTo> (AUTHOR)<i> jinyk@mail.ustc.edu.cn</i><br /><searchLink fieldCode="AR" term="%22Li%2C+Hui%22">Li, Hui</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> lihui@dhu.edu.cn</i><br /><searchLink fieldCode="AR" term="%22Liu%2C+Hao%22">Liu, Hao</searchLink><relatesTo>1</relatesTo> (AUTHOR)<i> liuh@dhu.edu.cn</i>
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  Data: <searchLink fieldCode="JN" term="%22Chinese+Journal+of+Polymer+Science+%28Springer+Science+%26+Business+Media+B%2EV%2E%29%22">Chinese Journal of Polymer Science (Springer Science & Business Media B.V.)</searchLink>. Nov2025, Vol. 43 Issue 11, p2030-2041. 12p.
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  Data: <searchLink fieldCode="DE" term="%22Click+chemistry%22">Click chemistry</searchLink><br /><searchLink fieldCode="DE" term="%22Reactive+polymers%22">Reactive polymers</searchLink><br /><searchLink fieldCode="DE" term="%22Genetic+techniques%22">Genetic techniques</searchLink><br /><searchLink fieldCode="DE" term="%22Nucleotide+sequencing%22">Nucleotide sequencing</searchLink><br /><searchLink fieldCode="DE" term="%22Individualized+medicine%22">Individualized medicine</searchLink><br /><searchLink fieldCode="DE" term="%22Biocompatibility%22">Biocompatibility</searchLink><br /><searchLink fieldCode="DE" term="%22Surface+preparation%22">Surface preparation</searchLink><br /><searchLink fieldCode="DE" term="%22Immobilized+nucleic+acids%22">Immobilized nucleic acids</searchLink>
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  Label: Abstract
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  Data: In this study, an amine-reactive poly(pentafluorophenyl acrylate) (PPFPA) platform was developed for advanced surface engineering of next-generation sequencing (NGS) chips. Through post-polymerization modification, PPFPA was functionalized with dual moieties: azide groups for covalent immobilization of DBCO-modified DNA primers via click chemistry and tunable hydrophilic side chains to optimize biocompatibility and surface properties. Systematic screening revealed that hydrophobic azide carriers combined with neutral hydroxyl groups maximized the DNA immobilization efficacy, approaching the performance of commercial polyacrylamide-based polymers. The negatively charged carboxyl groups severely impede DNA primer attachment. Higher molecular weight derivatives further enhance the efficacy of DNA immobilization. In NGS validation, optimized surface modification polymers achieved robust surface density of clustered DNA and high sequencing accuracy, surpassing quality benchmarks and comparable to those of conventional analogs. This platform demonstrates significant potential for tailoring high-sensitivity surfaces for genomic applications, advancing clinical diagnostics, and personalized medicine. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Chinese Journal of Polymer Science (Springer Science & Business Media B.V.) is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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RecordInfo BibRecord:
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      – Type: doi
        Value: 10.1007/s10118-025-3413-8
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      – Code: eng
        Text: English
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      Pagination:
        PageCount: 12
        StartPage: 2030
    Subjects:
      – SubjectFull: Click chemistry
        Type: general
      – SubjectFull: Reactive polymers
        Type: general
      – SubjectFull: Genetic techniques
        Type: general
      – SubjectFull: Nucleotide sequencing
        Type: general
      – SubjectFull: Individualized medicine
        Type: general
      – SubjectFull: Biocompatibility
        Type: general
      – SubjectFull: Surface preparation
        Type: general
      – SubjectFull: Immobilized nucleic acids
        Type: general
    Titles:
      – TitleFull: Amine-reactive Polymer Platform for Engineering Surface Modification of Next-generation Sequencing Chips.
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            NameFull: Tian, Wei
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            NameFull: Wang, Xin-Yuan
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            NameFull: Feng, Die-Wen
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            NameFull: Li, Xiang-Qian
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            NameFull: Jin, Yue-Kang
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            – D: 01
              M: 11
              Text: Nov2025
              Type: published
              Y: 2025
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              Value: 02567679
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              Value: 43
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            – TitleFull: Chinese Journal of Polymer Science (Springer Science & Business Media B.V.)
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