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Conserved spatial patterning of gene expression in independent lineages of C4 plants.

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Title: Conserved spatial patterning of gene expression in independent lineages of C4 plants.
Authors: Sun, Tianshu1 (AUTHOR), Bonthala, Venkata Suresh2 (AUTHOR), Stich, Benjamin2 (AUTHOR), Luginbuehl, Leonie H.1 (AUTHOR) lhl28@cam.ac.uk, Hibberd, Julian M.1 (AUTHOR) jmh65@cam.ac.uk
Source: New Phytologist. Jan2026, Vol. 249 Issue 1, p24-38. 15p.
Subjects: Carbon 4 photosynthesis, Gene expression, Transcription factors, Plant cells & tissues, Transcriptomes, RNA sequencing
Abstract: Summary: C4 photosynthesis enhances carbon fixation efficiency by reducing photorespiration through the use of an oxygen‐insensitive carboxylase and spatial separation of photosynthesis between mesophyll and bundle sheath cells. The C4 pathway has evolved independently in > 60 plant lineages, but molecular mechanisms underpinning this convergence remain unclear. To explore this, we generated high‐resolution transcriptome atlases for two independently evolved C4 dicotyledonous species – Gynandropsis gynandra (NAD‐malic enzyme subtype) and Flaveria bidentis (NADP‐malic enzyme subtype). We used both single‐cell and single‐nucleus RNA sequencing to capture gene expression profiles from individual leaf cells, enabling a detailed comparison between cell types and transcriptional signatures. While both approaches produced biologically comparable data for major leaf cell types, transcriptomes from single‐nucleus sequencing showed lower stress signatures and were more representative of tissue proportions in the leaf. The single‐nucleus data revealed that bundle sheath cells from both C4 species share a gene expression pattern associated with mesophyll cells of C3 plants. A conserved set of transcription factors, including members of the C2H2 and DOF families, was identified in the bundle sheath cells of both species. This study presents the first single‐cell‐resolution transcriptomes for two independent C4 dicot lineages and provides a valuable resource, including a web‐based portal for data visualisation. See also the Commentary on this article by Yee & Furbank, 249: 5–7. [ABSTRACT FROM AUTHOR]
Copyright of New Phytologist is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
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DbLabel: Engineering Source
An: 189830575
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
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  Data: Conserved spatial patterning of gene expression in independent lineages of C<subscript>4</subscript> plants.
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  Data: <searchLink fieldCode="JN" term="%22New+Phytologist%22">New Phytologist</searchLink>. Jan2026, Vol. 249 Issue 1, p24-38. 15p.
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  Data: <searchLink fieldCode="DE" term="%22Carbon+4+photosynthesis%22">Carbon 4 photosynthesis</searchLink><br /><searchLink fieldCode="DE" term="%22Gene+expression%22">Gene expression</searchLink><br /><searchLink fieldCode="DE" term="%22Transcription+factors%22">Transcription factors</searchLink><br /><searchLink fieldCode="DE" term="%22Plant+cells+%26+tissues%22">Plant cells & tissues</searchLink><br /><searchLink fieldCode="DE" term="%22Transcriptomes%22">Transcriptomes</searchLink><br /><searchLink fieldCode="DE" term="%22RNA+sequencing%22">RNA sequencing</searchLink>
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  Data: Summary: C4 photosynthesis enhances carbon fixation efficiency by reducing photorespiration through the use of an oxygen‐insensitive carboxylase and spatial separation of photosynthesis between mesophyll and bundle sheath cells. The C4 pathway has evolved independently in > 60 plant lineages, but molecular mechanisms underpinning this convergence remain unclear. To explore this, we generated high‐resolution transcriptome atlases for two independently evolved C4 dicotyledonous species – Gynandropsis gynandra (NAD‐malic enzyme subtype) and Flaveria bidentis (NADP‐malic enzyme subtype). We used both single‐cell and single‐nucleus RNA sequencing to capture gene expression profiles from individual leaf cells, enabling a detailed comparison between cell types and transcriptional signatures. While both approaches produced biologically comparable data for major leaf cell types, transcriptomes from single‐nucleus sequencing showed lower stress signatures and were more representative of tissue proportions in the leaf. The single‐nucleus data revealed that bundle sheath cells from both C4 species share a gene expression pattern associated with mesophyll cells of C3 plants. A conserved set of transcription factors, including members of the C2H2 and DOF families, was identified in the bundle sheath cells of both species. This study presents the first single‐cell‐resolution transcriptomes for two independent C4 dicot lineages and provides a valuable resource, including a web‐based portal for data visualisation. See also the Commentary on this article by Yee & Furbank, 249: 5–7. [ABSTRACT FROM AUTHOR]
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  Data: <i>Copyright of New Phytologist is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Value: 10.1111/nph.70475
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        Text: English
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        Type: general
      – SubjectFull: Gene expression
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      – SubjectFull: Transcription factors
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      – SubjectFull: Plant cells & tissues
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      – SubjectFull: Transcriptomes
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      – SubjectFull: RNA sequencing
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      – TitleFull: Conserved spatial patterning of gene expression in independent lineages of C4 plants.
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              Text: Jan2026
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